1042 HEPATOCELLULAR CARCINOMA REPLICATING HEPATITIS B VIRUS HAVE TRANSCRIPTOMIC, HISTOLOGICAL AND CLINICAL CHARACTERISTICS

Results: Approximately 2 million, 76bp reads were randomly generated per sample, providing 5% coverage of the human genome. CNAs were identified and showed no significant aberrations in Macroregenerative nodules (MRN) (n =8). Dysplastic nodules (DN) (n =5) showed development of spikes of amplifications and deletions, which became increasingly complex and involved larger areas of the chromosome as HCC developed (n =22). This progression was demonstrated on chromosomes 1, 5, 7, 14, 22 for amplifications and 1, 4, 6, 14, 16, 22 for deletions (figure 1). Conclusion: NGS can provide CNA data from FFPE material with reasonable costs (£50/sample). Genetic profiles of pre-malignant nodules revealed no changes within MRNs. However lesions start to occur with the onset of dysplasia and become increasingly complex. Initial amplifications and deletions expanded in size with progression. More work is needed to expand cohorts and investigate heterogeneity within individual lesions.