Fabrication of an Amperometric Triglyceride Biosensor based on PVC Membrane

An amperometric enzyme sensor for determination of triglycerides (TGs) was constructed by mounting polyvinyl chloride (PVC) membrane bound lipase, glycerol kinase (GK), glycerol-3-phosphate oxidase (GPO), and horseradish peroxidase (HRP) on a platinum electrode (working electrode), then connecting it to electrometer along with Ag/AgCl reference electrode and Cu auxiliary electrode. The biosensor measures the electrons generated from H2O2 at 0.4 V, which is formed from triolein/triglycerides by co-immobilized lipase, GK, GPO, and HRP. The concentration of triolein/TG was directly proportional to the current measured. The enzyme electrode showed optimum response when operated at 25°C in 0.1 M sodium phosphate buffer, pH 7.5, for 30 s. A linear relationship was obtained between triolein concentration ranging from 0.56 to 2.25 mM and amount of current (mA). The minimum detection limit of the method was 0.11 mM. The levels of TG in serum of apparently healthy persons and persons suffering from cardiovascular d...