Long non‑coding RNA RP11‑400N13.3 promotes the progression of colorectal cancer by regulating the miR‑4722‑3p/P2RY8 axis
2020
Accumulating evidence has shown that long noncoding RNAs (lncRNAs) play significant roles in the development and progression of many types of cancer including colorectal cancer. RP11400N13.3 is a novel lncRNA discovered recently and its biological function and underlying mechanism in colorectal cancer remain elusive. This study aimed to reveal the relationship between RP11400N13.3 and colorectal cancer. Our results demonstrated that the expression of RP11400N13.3 was significantly upregulated in both colorectal cancer tissues and cell lines as compared to normal adjacent tissues and normal colonic epithelial cells by RTqPCR, respectively. Upregulation of RP11400N13.3 was found to be correlated with a poor overall survival rate. Functional studies revealed that RP11400N13.3 facilitated the proliferation, migration, invasion and tumor growth of colorectal cancer cells while inhibiting the apoptosis of cancer cells in vitro and in vivo. We also observed that RP11400N13.3 serves as a sponge for miR47223p, and that P2Y receptor family member 8 (P2RY8) was predicted to be a target of miR47223p by bioinformatics analysis. Western blot assay indicated that the expression of P2RY8 was negatively or positively regulated by miR47223p or RP11400N13.3. In addition, rescue experiments revealed that RP11400N13.3 promoted proliferation, migration and invasion by directly regulating the expression of miR47223p and P2RY8. In conclusion, our results revealed that RP11400N13.3 promoted colorectal cancer progression via modulating the miR47223p/P2RY8 axis, thus suggesting RP11400N13.3 as a potential therapeutic target for the treatment of colorectal cancer.
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