Development and Validation of Simple HPLC Method for Estimation of Flubendiamide in Blood

Flubendiamide, novel insecticide used for control of lepidopteron insects on agriculture crops in India. Flubendiamide exposure of to human and animals occurs through ingestion of contaminated feed/fodder, food-products and drinking water etc. Aim of the present study was to develop a simple HPLC method for flubendiamide estimation in blood plasma. Result indicated that HPLC estimation of flubendiamide in blood plasma shows linear standard curve in the range of 0.039-5.0 μg.ml -1 with correlation coefficient (R 2 ) 0.999. Limit of Detection (LOD) and Limit of Quantification (LOQ) was 0.039 μgml -1 . Intra-day, inter-day coefficient of variance and mean recovery were found to be 2.70%, 3.50% and approx. 100% respectively, by using C 18 column and mixture of acetonitrile: water (70:30, v/v) as mobile phase with flow rate of 0.5 mlmin -1 at 210 λ. Result of this study concluded that this HPLC method is very simple, reliable and repeatable for flubendiamide estimation blood plasma.