Restriction enzyme/multi-locus probe combinations useful for DNA fingerprinting of the striped bass, white bass and their F1 hybrid

Abstract DNA fingerprints were produced in striped bass ( Morone saxatilis ), white bass ( Morone chrysops ) and their F1 hybrid to identify useful restriction enzyme/probe combinations, determine within- and between-genetic group bandsharing and identify allelic and linkage relationships among fingerprint bands. Five restriction enzymes ( Alu I, Hae III, Hinf I, Sau 3A and Dra I) and three multi-locus probes (33.6, 33.15 and R18.1) were screened. Alu I/33.15, Alu I/R18.1, Hinf I/33.15 and Hinf I/R18.1 were identified as the best enzyme/probe combinations. Within-group bandsharings calculated using these combinations ranged from 0.19 to 0.35 for striped bass, 0.39 to 0.65 for white bass and 0.34 to 0.45 for their hybrid. Between-group bandsharing ranged from 0.12 to 0.26 for striped bass vs. white bass, 0.27 to 0.47 for striped bass vs. hybrid and 0.24 to 0.46 for white bass vs. hybrid. Pedigree analysis of a full-sib, F1 hybrid family indicated no bands were linked, allelic pairs were present in the dam (striped bass) and no allelic pairs were present in the sire (white bass). Potential uses of DNA fingerprinting in Morone breeding programs are discussed.