Glucan binding domain of streptococcal glucosyltransferases

, R. R. B., Glucan binding domain of streptococ-cal glucosyltransferases. Biologia, Bratislava,57/Suppl. 11: 129|136, 2002;ISSN 0006-3088.Glucosyltransferase I (GTF-I) of Streptococcus downei synthesises an insol-uble -1,3-linked glucan from sucrose. It contains a catalytic domain that isa circularly-permuted homologue of the catalytic domain of Family 13 en-zymes and a C-terminal glucan-binding domain (GBD) that consists of aseries of tandem repeats, each ˘35 amino acids long. In order to examinethe contribution of these repeats to binding, a nested series of plasmids wasconstructed that expressed truncated forms of GTF-I or GBD in Escherichiacoli,withanN-terminalHis