Bacillus subtilis preparation and preparation method thereof

The invention discloses a bacillus subtilis preparation and preparation method thereof. The bacillus subtilis preparation is prepared by bacillus subtilis fermentation broth prepared by the following steps: accessing bacillus subtilis seed liquid into fermentation medium with 0.5 to 5% (weight ratio) of inoculation amount, and conducting fermentation for 20 to 40 hours, with pressure being 0.02 to 0.15 MPa, agitation speed being 100 to 300 r/m, and ventilation ratio being 1:0.3-1.5(volume ratio). The advantages of the invention: the invention adopts liquid deep fermentation method instead of solid fermentation method to improve efficiency of preparation technology, and further optimizes composition of the mediums used in various steps and fermentation technological conditions to substantially increase live bacteria content of the bacillus subtilis in final product; the bacillus subtilis preparation is acid resistant and cholate resistant, and keeps high efficiency and stability in intestinal tract environment; and gemma formation rate is high, the bacillus subtilis is more high-temperature resistant and convenient for processing and preservation.