Lymphatic endothelium stimulates melanoma metastasis and invasion via MMP14-dependent Notch3 and β1-integrin activation

The death rates for many types of cancer have dropped, but melanoma remains a serious concern. This type of skin cancer is especially aggressive because it can spread to distant organs. Melanoma often spreads via the lymphatic system, a network of vessels that extends throughout the body to drain fluid from the body’s tissues. The lymphatic system also includes structures – called lymph nodes – that filter bacteria from this fluid; this helps to defend against infection. When melanoma spreads to lymph nodes and distant organs, clinicians diagnose it as Stage IV melanoma. For patients at this stage, the outcome is often poor. It is clear that melanoma exploits lymph vessels to spread throughout the body. But researchers also suspect that vessel cells interact with the cancer cells, helping the melanoma invade distant organs. Understanding exactly how lymph vessels promote the spread of melanoma will lead to better options for treating this aggressive cancer. Pekkonen, Alve et al. investigated whether exposing human melanoma cells to cells from the walls of human lymph vessels would make the cancer cells more aggressive. Indeed, after growing the two cell types together in the laboratory, the melanoma cells became more invasive. When transplanted into mice, these cancer cells spread to and invaded the rodents’ distant organs. Pekkonen, Alve et al. conducted a series of experiments to identify specific proteins in the melanoma cellsthat were responsible for making the cancer more invasive after it interacted with the lymph vessel cells. These experiments identified proteins called MMP14, Notch3, and β1-integrin as critical to the invasive spread of melanoma cells. When melanoma cells with less MMP14 or Notch3 were implanted into zebrafish, the cancer cells spread less efficiently. These findings may represent new leads that clinicians can test to see if they are markers of cancers that are most likely to spread and that the pharmaceutical industry can pursue to treat melanoma patients.