Inhibition Of Oxidative Phosphorylation In Medullary Thyroid Cancer Cells Affects mRNA Levels Of Genes Controlling Cancer Cell Proliferation And Apoptosis.

Objectives: Most pediatric medullary thyroid cancers (MTC) result from dominantly inherited or de novo activating mutations in the Rearranged-during-transfection (RET) proto-oncogene.(1) Our group previously demonstrated that RET-positive MTC-derived cells have significantly increased mitochondrial respiratory chain molecules as compared to normal thyroid tissue. In this study, we examined how inhibition of oxidative phosphorylation affects expression of genes controlling response to oxidative stress and DNA damage in MTC-derived cells. Methods: Human MTC-derived TT cells (harboring C634W RET mutation) were grown in RPMI 1640 …