Preparation of β-CD-quercetin complex and its effects on ethanol-injuried BRL-3A hepatocytes.

OBJECTIVE Quercetin was parcelled by β-cyclodextrin (β-CD) to prepare the sustained-release complex, and the effect of β-CD-quercetin complex on the growth of ethanol-injuried hepatocytes was studied. METHODS By using scanning electron microscopy, infrared spectroscopy, and release rate analysis, β-CD-quercetin complex was identified. The effect of different concentrations of β-CD-quercetin complex on ethanol-injuried hepatocyte growth at different time was observed by using MTT assay, and the cell quantity and morphology were observed by using hematoxylin-eosin staining. By using single-cell gel electrophoresis, the prevention of β-CD-quercetin complex on the DNA damage of ethanol-injuried BRL-3A cells was explored, and Olive tail moment was calculated. RESULTS β-CD-quercetin complex as sustained-release complex was successfully prepared. The ethanol damage of BRL-3A cells could be prevented by 20, 40 and 80 mg/L of quercetin complex, and the protection mechanism of hepatocyte was related to the antioxidation of DNA. CONCLUSION Quercetin sustained-release complex could be prepared with β-CD, and they might be used to treat alcoholic liver injury.