Loop-mediated isothermal amplification for detection of Aeromonas hydrophila and A. sobria

The study established a loop-mediated isothermal amplification ( LAMP) technology for Aeromonas hydrophila and A. sobria. We designed the primers based on the sequences of the pilin gene of A. hydrophila and the zipA gene of A. sobria, respectively, and conducted specificity and sensitivity tests by real-time turbidimeter under isothermal conditions, which were detected by agarose gel electrophoresis test and change of SYBR Green I colour. The results show that the LAMP method was effective for rapid detection of A. hydrophila and A. sobria with limit of detections of 46 fg·mL 1 and 320 fg·mL 1 , 10 and 10 times more sensitive than the conventional PCR, respectively. In conclusion, the LAMP detective method for A. hydrophila and A. sobria which we established was specific, sensitive, effective and rapid.