Effect of sucrose concentration on photosynthetic activity of in vitro cultures Gentiana kurroo (Royle) germlings

In this work, the effect of sucrose on photosynthetic activity during in vitro culture was studied. Experiments were carried out using uniform somatic embryo-derived germlings of Gentiana kurroo (Royle) confirmed by chromosome counting and flow cytometry technique. Photosynthetic activity was measured by chlorophyll a fluorescence and gas exchange method. The efficiency of photosynthetic apparatus as measured by the ratio F v/F m, Yield and qP (light phase of photosynthesis) was the highest when the medium was supplemented with 0.3% sucrose which well corresponded with plant gas exchange. Taking all data into consideration for the best development of photosynthetic apparatus and the most efficient of net photosynthesis of studied germlings would be medium supplemented with 0.2–0.4% of sucrose.