Effect of bacteriophage lysin on lysogens

Abstract Objective To study the effect of phage lysin on the growth of lysogens. Methods Sputum specimens processed by modified Petroff's method were respectively treated with phagebiotics in combination with lysin and lysin alone. The specimens were incubated at 37 °C for 4 days. At the end of day 1, 2, 3 and day 4, the specimens were streaked on blood agar plates and incubated at 37 °C for 18–24 hours. The growth of normal flora observed after day 1 was considered as lysogens. Results Sputum specimens treated with phagebiotics-lysin showed the growth of lysogens. When specimens treated with lysin alone, lysogen formation was avoided and normal flora was controlled. Conclusions Lysin may have no effect on the growth of lysogens.