Solubleform of (pro)renin receptor is excreted from mesothelial cells to peritoneal dialysis effluent of peritoneal dialysis patients (1173.5)

Renin angiotensin system (RAS) is associated with peritoneal injury in peritoneal dialysis (PD). Angiotensin II dependent and independent role of (pro) renin receptor ((P)RR) to the cellular injury has been demonstrated. Thus, the present study determined the expression of (P)RR in drained PD effluent (PDE) and human peritoneal mesothelial cells (HPMCs) of the PD patients. PDE drained after 2.5% glucose PD solution during peritoneal equivalent test (0hours, 2hours, 4hours) was collected from PD patients. Human soluble from of (P)RR level was measured by ELISA. The expression of (P)RR on cultured HPMCs and media isolated from PDE were investigated by Western blot analysis and/or flow cytometry. HPMCs was specified by cytokeratin positive cells in flow cytometry. Full length or soluble (P)RR was identified by the molecular weight compared to the full length and soluble (P)RR synthesized in vitro from the expression vectors. Soluble (P)RR was detected in both plasma and PDE of PD patients. Time dependent inc...