Bufalin, is a cardiotonic steroid originally isolated from the Chinese toad venom. It is a component found in many Chinese traditional medicine. Bufalin, is a cardiotonic steroid originally isolated from the Chinese toad venom. It is a component found in many Chinese traditional medicine. Bufalin has a in vitro antitumor effects against various malignancies, including hepatocellular and lung carcinoma.However, as with other bufadienolides, its use is hampered by its cardiotoxicity.