In enzymology, an acetylspermidine deacetylase (EC 3.5.1.48) is an enzyme that catalyzes the chemical reaction In enzymology, an acetylspermidine deacetylase (EC 3.5.1.48) is an enzyme that catalyzes the chemical reaction Thus, the two substrates of this enzyme are N8-acetylspermidine and H2O, whereas its two products are acetate and spermidine. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N8-acetylspermidine amidohydrolase. Other names in common use include N8-monoacetylspermidine deacetylase, N8-acetylspermidine deacetylase, N-acetylspermidine deacetylase, N1-acetylspermidine amidohydrolase (incorrect), and 8-N-acetylspermidine amidohydrolase.