Genetic assimilation

Genetic assimilation is a process by which a phenotype originally produced in response to an environmental condition, such as exposure to a teratogen, later becomes genetically encoded via artificial selection or natural selection. Despite superficial appearances, this does not require the (Lamarckian) inheritance of acquired characters, although epigenetic inheritance could potentially influence the result. Genetic assimilation overcomes the barrier to selection imposed by genetic canalization of developmental pathways. Genetic assimilation is a process by which a phenotype originally produced in response to an environmental condition, such as exposure to a teratogen, later becomes genetically encoded via artificial selection or natural selection. Despite superficial appearances, this does not require the (Lamarckian) inheritance of acquired characters, although epigenetic inheritance could potentially influence the result. Genetic assimilation overcomes the barrier to selection imposed by genetic canalization of developmental pathways. The classic example of genetic assimilation was a pair of experiments in 1942 and 1953 by Conrad H. Waddington, in which Drosophila fruit fly embryos were exposed to ether, producing a bithorax-like phenotype (a homeotic change). Flies which developed halteres (the modified hindwings of true flies, used for balance) with wing-like characteristics were chosen for breeding for 20 generations, by which point the phenotype could be seen without other treatment. More recent evidence appears to confirm the existence of genetic assimilation in evolution. Conrad H. Waddington's classic experiment (1942) induced an extreme environmental reaction in the developing embryos of Drosophila. In response to ether vapor, a proportion of embryos developed a radical phenotypic change, a second thorax. At this point in the experiment bithorax is not innate; it is induced by an unusual environment. Waddington then repeatedly selected Drosophila for the bithorax phenotype over some 20 generations. After this time, some Drosophila developed bithorax without the ether treatment. Waddington carried out a similar experiment in 1953, this time inducing the cross-veinless phenocopy in Drosophila with a heat shock, with 40% of the flies showing the phenotype prior to selection. Again he selected for the phenotype over several generations, applying heat shock each time, and eventually the phenotype appeared even without heat shock. Waddington called the effect he had seen genetic assimilation. His explanation was that it was caused by a process he called canalization. He compared embryonic development to a ball rolling down a slope in what he called an epigenetic landscape, where each point on the landscape is a possible state of the organism (involving many variables). As a particular pathway becomes entrenched or canalized, it becomes more stable, likely to occur even in the face of environmental changes. Major perturbations such as ether or heat shock eject the developmental pathway from the metaphorical canal, exploring other parts of the epigenetic landscape. Selection in the presence of that perturbation leads to the evolution of a new canal; after the perturbation is discontinued, the new canal continues to attract developmental trajectories. Other biologists have agreed that assimilation occurs, but give a different, purely quantitative genetics explanation in terms of natural or artificial selection. The phenotype, say cross-veinless, is presumed to be caused by a combination of multiple genes. The phenotype appears when the sum of gene effects exceeds a threshold that is lower with the perturbation than without. Continued selection under perturbation conditions increases the frequency of the alleles of genes that promote the phenotype until the higher threshold is breached, and the phenotype appears without requiring the heat shock. Perturbations can be genetic or epigenetic rather than environmental. For example, Drosophila fruit flies have a heat shock protein, Hsp90, which protects the development of many structures in the adult fly from heat shock. If the protein is damaged by a mutation, then just as if it were damaged by the environmental effects of drugs, many different phenotypic variants appear; if these are selected for, they quickly establish without further need for the mutant Hsp90. Waddington's theory of genetic assimilation was controversial. The evolutionary biologists Theodosius Dobzhansky and Ernst Mayr both thought that Waddington was using genetic assimilation to support so-called Lamarckian inheritance. They denied that the inheritance of acquired characteristics had taken place, and asserted that Waddington had simply observed the natural selection of genetic variants that already existed in the study population. Waddington himself interpreted his results in a Neo-Darwinian way, particularly emphasizing that they 'could bring little comfort to those who wish to believe that environmental influences tend to produce heritable changes in the direction of adaptation.' Adam S. Wilkins wrote that ' in his lifetime... was widely perceived primarily as a critic of Neo-Darwinian evolutionary theory. His criticisms ... were focused on what he saw as unrealistic, 'atomistic' models of both gene selection and trait evolution.' In particular, according to Wilkins, Waddington felt that the Neo-Darwinians badly neglected the phenomenon of extensive gene interactions and that the 'randomness' of mutational effects, posited in the theory, was false. Even though Waddington became critical of the neo-Darwinian synthetic theory of evolution, he still described himself as a Darwinian, and called for an extended evolutionary synthesis based on his research. Waddington did not deny the threshold-based conventional genetic interpretation of his experiments, but regarded it 'as a 'told to the children' version of what I wished to say' and considered the debate to be about 'mode of expression, rather than of substance'.