NLRP1

1PN5, 3KAT, 4IFP, 4IM622861637515ENSG00000091592ENSMUSG00000070390Q9C000Q2LKV5Q2LKV2Q2LKW6Q0GKD5NM_033007NM_001033053NM_014922NM_033004NM_033006NM_001039680NM_001040696NM_001162414NP_001028225NP_055737NP_127497NP_127499NP_127500NP_001035786NP_001155886NLRP1 encodes NACHT, LRR, FIIND, CARD domain and PYD domains-containing protein 1 in humans . NLRP1 was the first protein shown to form an inflammasome . NLRP1 is expressed by a variety of cell types, which are predominantly hematopoietic. The expression is also seen within glandular epithelial structures including the lining of the small intestine, stomach . NLRP1 polymorphisms are associated with skin extra-intestinal manifestations in CD . Its highest expression was detected in human skin, in psoriasis and in vitiligo. Polymorphisms of NLRP1 were found in lupus erythematosus and diabetes type 1. Variants of mouse NLRP1 were found to be activated upon N-terminal cleavage by the protease in anthrax lethal factor . NLRP1 encodes NACHT, LRR, FIIND, CARD domain and PYD domains-containing protein 1 in humans . NLRP1 was the first protein shown to form an inflammasome . NLRP1 is expressed by a variety of cell types, which are predominantly hematopoietic. The expression is also seen within glandular epithelial structures including the lining of the small intestine, stomach . NLRP1 polymorphisms are associated with skin extra-intestinal manifestations in CD . Its highest expression was detected in human skin, in psoriasis and in vitiligo. Polymorphisms of NLRP1 were found in lupus erythematosus and diabetes type 1. Variants of mouse NLRP1 were found to be activated upon N-terminal cleavage by the protease in anthrax lethal factor . This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. The NLRP1 protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined . NLRP1 activates a antibacterial immune response. Antibacterial immune response compensates for the loss of the MAP kinase response. Humans produce NLRP1, but human NLRP1 is activated by lethal factor . NLRP1 could be activated by proteolytic cleavage resulting in the removal of an auto-inhibitory PYD . But the mechanism of human NLRP1 activation and the function in immunity is not unclear . NLRP1 has been shown to interact with caspase 9 and APAF1 . Mice have three paralogs of the Nlrp1 gene (Nlrp1a, b, c). Nlrp1c is a pseudogene . Mouse NLRP1B is not activated by a receptor-ligand type mechanism. NLRP1B variants from certain inbred mouse strains, BALB/c and 129, can be activated by the lethal factor (LF) protease. The lethal factor protease is produced and secreted by Bacillus anthracis, the agent of anthrax . Together with protective antigen (PA), LF forms a bipartite toxin, Lethal Toxin. The role of PA is to form a translocation channel that delivers LF into the host cell cytosol, where LF play roles in immune response by cleaving and inactivating MAP kinases. LF also directly cleaves NLRP1B proximal to its N-terminus, it is necessary and sufficient for NLRP1B inflammasome formation and CASP1 activation . Activation of NLRP1B-dependent inflammasome responses appears in host defense with mechanism like IL-1β and neutrophils . NLRP1B can function as a sensor of bacterial proteases, immune responses are specifically activated by virulence factors . It is not clear what stimuli might activate NLRP1A, the other known functional murine NLRP1 paralog. The study identified a mouse carrying a missense gain-of-function mutation in NLRP1A (Q593P) that active inflammasome responses. The mechanism of wild-type NLRP1A activation is unclear .