beta-Sitosterol

β-Sitosterol (beta-sitosterol) is one of several phytosterols (plant sterols) with chemical structures similar to that of cholesterol. It is a white, waxy powder with a characteristic odor, and is one of the components of E499. Phytosterols are hydrophobic and soluble in alcohols. β-Sitosterol (beta-sitosterol) is one of several phytosterols (plant sterols) with chemical structures similar to that of cholesterol. It is a white, waxy powder with a characteristic odor, and is one of the components of E499. Phytosterols are hydrophobic and soluble in alcohols. Beta-sitosterol is widely distributed in the plant kingdom and found in vegetable oil, nuts, avocados and prepared foods, such as salad dressings. β-sitosterol is being studied for its potential to reduce benign prostatic hyperplasia (BPH) and blood cholesterol levels. High levels of β-sitosterol concentrations in blood have been correlated with increased severity of heart disease in men having previously suffered from heart attacks. While plant sterols are usually beneficial, there is a rare autosomal recessive genetic disorder phytosterolemia which causes over-absorption of phytosterols. Being a steroid, β-sitosterol is precursor of anabolic steroid boldenone. Boldenone undecylenate is commonly used in veterinary medicine to induce growth in cattle but it is also one of the most commonly abused anabolic steroids in sports. This led to suspicion that some athletes testing positive on boldenone undecylenate didn't actually abuse the hormone itself but consumed food rich in β-sitosterol. The use of sitosterol as a chemical intermediate was for many years limited due to the lack of a chemical point of attack on the side-chain that would permit its removal. Extensive efforts on the part of many laboratories eventually led to the discovery of a pseudomonas microbe that efficiently effected that transformation. Fermentation digests the entire aliphatic side-chain at carbon 17 to afford a mixture of 17-keto products including dehydroepiandrosterone. Total synthesis of β-sitosterol has not been achieved. However, β-sitosterol has been synthesized from stigmasterol 1, which involves a specific hydrogenation of the side-chain of stigmasterol (See Figure Below). The first step in the synthesis forms stigmasterol tosylate 2 from stigmasterol 1 (95% purity) using p-TsCl, DMAP, and pyridine (90% yield). The tosylate 2 then undergoes solvolysis as it is treated with pyridine and anhydrous MeOH to give a 5:1 ratio of i-stigmasterol methyl ether 3 (74% yield) to stigmasterol methyl ether 4, which is subsequently removed by chromatography. The hydrogenation step of a previously proposed synthesis involved the catalyst Pd/C and the solvent ethyl acetate. However, due to isomerisation during hydrolysis, other catalysts, such as PtO2, and solvents, such as ethanol, were tested. There was little change with the use of a different catalyst. Ethanol, however, prevented isomerisation and the formation of the unidentified impurity to give compound 5. The last step of the synthesis is deprotection of the β-ring double bond of 5 with p-TsOH, aqueous dioxane, and heat (80 °C) to yield β-sitosterol 6. The cumulative yield for the final two steps was 55%, and the total yield for the synthesis was 37%. The regulation of the biosynthesis of both sterols and some specific lipids occurs during membrane biogenesis. Through 13C-labeling patterns, it has been determined that both the mevalonate and deoxyxylulose pathways are involved in the formation of β-sitosterol. The precise mechanism of β-sitosterol formation varies according to the organism, but is generally found to come from cycloartenol.