language-icon Old Web
English
Sign In

Telophase

Telophase (from the Greek τέλος (télos), 'end' and φάσις (phásis), 'stage') is the final stage in both meiosis and mitosis in a eukaryotic cell. Telophase (from the Greek τέλος (télos), 'end' and φάσις (phásis), 'stage') is the final stage in both meiosis and mitosis in a eukaryotic cell. During telophase, the effects of prophase and prometaphase (the nuclear membrane and nucleolus disintegrating) are reversed. As chromosomes reach the cell poles, a nuclear envelope is re-assembled around each set of chromatids, the nucleoli reappear, and chromosomes begin to decondense back into the expanded chromatin that is present during interphase. The mitotic spindle is disassembled and remaining spindle microtubules are depolymerized. Telophase accounts for approximately 2% of the cell cycle's duration. Cytokinesis typically begins before late telophase and, when complete, segregates the two daughter nuclei between a pair of separate daughter cells. Telophase is primarily driven by the dephosphorylation of mitotic cyclin-dependent kinase (Cdk) substrates. The phosphorylation of the protein targets of M-Cdks (Mitotic Cyclin-dependent Kinases) drives spindle assembly, chromosome condensation and nuclear envelope breakdown in early mitosis. The dephosphorylation of these same substrates drives spindle disassembly, chromosome decondensation and the reformation of daughter nuclei in telophase. Establishing a degree of dephosphorylation permissive to telophase events requires both the inactivation of Cdks and the activation of phosphatases. Cdk inactivation is primarily the result of the destruction of its associated cyclin. Cyclins are targeted for proteolytic degradation by the anaphase promoting complex (APC), also known as the cyclosome, a ubiquitin-ligase. The active, CDC20-bound APC (APC/CCDC20) targets mitotic cyclins for degradation starting in anaphase. Experimental addition of non-degradable M-cyclin to cells induces cell cycle arrest in a post-anaphase/pre-telophase-like state with condensed chromosomes segregated to cell poles, an intact mitotic spindle, and no reformation of the nuclear envelope. This has been shown in frog (Xenopus) eggs, fruit flies (Drosophilla melanogaster), budding (Saccharomyces cerevisiae) and fission (Schizosaccharomyces pombe) yeast, and in multiple human cell lines. The requirement for phosphatase activation can be seen in budding yeast, which do not have redundant phosphatases for mitotic exit and rely on the phosphatase cdc14. Blocking cdc14 activation in these cells results in the same phenotypic arrest as does blocking M-cyclin degradation. Historically, it has been thought that anaphase and telophase are events that occur passively after satisfaction of the spindle-assembly checkpoint (SAC) that defines the metaphase-anaphase transition. However, the existence of differential phases to cdc14 activity between anaphase and telophase is suggestive of additional, unexplored late-mitotic checkpoints. Cdc14 is activated by its release into the nucleus, from sequestration in the nucleolus, and subsequent export into the cytoplasm. The Cdc-14 Early Anaphase Release pathway, which stabilizes the spindle, also releases cdc14 from the nucleolus but restricts it to the nucleus. Complete release and maintained activation of cdc14 is achieved by the separate Mitotic Exit Network (MEN) pathway to a sufficient degree (to trigger the spindle disassembly and nuclear envelope assembly) only after late anaphase. Cdc14-mediated dephosphorylation activates downstream regulatory processes unique to telophase. For example, the dephosphorylation of CDH1 allows the APC/C to bind CDH1. APC/CCDH1 targets CDC20 for proteolysis, resulting in a cellular switch from APC/CCDC20 to APC/CCDH1 activity. The ubiquitination of mitotic cyclins continues along with that of APC/CCDH1-specific targets such as the yeast mitotic spindle component, Ase1, and cdc5, the degradation of which is required for the return of cells to the G1 phase.

[ "Interphase", "Cell cycle", "Prophase", "Metaphase", "Meiosis" ]
Parent Topic
Child Topic
    No Parent Topic