IRF1

1IF1365916362n/aENSMUSG00000018899P10914P15314NM_002198NM_001159393NM_001159396NM_008390NP_002189NP_001341853NP_001341854NP_001152865NP_001152868NP_032416Interferon regulatory factor 1 is a protein that in humans is encoded by the IRF1 gene.1if1: INTERFERON REGULATORY FACTOR 1 (IRF-1) COMPLEX WITH DNA Interferon regulatory factor 1 is a protein that in humans is encoded by the IRF1 gene. Interferon regulatory factor 1 was the first member of the interferon regulatory transcription factor (IRF) family identified. Initially described as a transcription factor able to activate expression of the cytokine Interferon beta, IRF-1 was subsequently shown to function as a transcriptional activator or repressor of a variety of target genes. IRF-1 regulates expression of target genes by binding to an interferon stimulated response element (ISRE) in their promoters. The IRF-1 protein binds to the ISRE via an N-terminal helix-turn-helix DNA binding domain, which is highly conserved among all IRF proteins. Beyond its function as a transcription factor, IRF-1 has also been shown to trans-activate the tumour suppressor protein p53 through the recruitment of its co-factor p300. IRF-1 has been shown to play roles in the immune response, regulating apoptosis, DNA damage and tumor suppression. It has been shown that the extreme C-terminus of IRF-1 regulates its ability to activate transcription, nanobodies targeting this domain (MF1) are able to increase IRF-1 activity. Model organisms have been used in the study of IRF1 function. A conditional knockout mouse line, called Irf1tm1a(EUCOMM)Wtsi was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute. Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Twenty five tests were carried out and two phenotypes were reported. Homozygous mutant animals had abnormal peripheral blood lymphocytes, specifically decreased CD8-positive T cell and NK cell numbers and an increase in CD4-positive T cells. The mice also had an abnormal integument phenotype determined by a study of tail epidermis. IRF1 has been shown to interact with: This article incorporates text from the United States National Library of Medicine, which is in the public domain.