The gonadosomatic index, abbreviated as GSI, is the calculation of the gonad mass as a proportion of the total body mass. It is represented by the formula:GSI = × 100 The gonadosomatic index, abbreviated as GSI, is the calculation of the gonad mass as a proportion of the total body mass. It is represented by the formula: It is a tool for measuring the sexual maturity of animals in correlation to ovary development and testes development. This index is frequently used as reporting point in OECD test guideline which may be used as indication or evidence of potential endocrine disruption effect of chemicals in regulatory frame work (EFSA and ECHA, 2017) WHO/IPCS