Mesodinium rubrum

Myrionecta rubra (or Mesodinium rubrum) is a species of ciliates. It constitutes a plankton community and is found throughout the year, most abundantly in spring and fall, in coastal areas. Although discovered in 1908, its scientific importance came into light in the late 1960s when it attracted scientists by the recurrent red colouration it caused by forming massive blooms, that cause red tides in the oceans. Unlike typical protozoans, M. rubra can make its own nutrition by photosynthesis. The unusual autotrophic property was discovered in 2006 when genetic sequencing revealed that the photosynthesising organelles, plastids, were derived from the principal food of the ciliate, the photosynthetic algae called cryptomonads (or cryptophytes). It is, thus, both autotrophic and heterotrophic. This nature also indicates that it is an example of endosymbiosis, supporting the endosymbiotic theory, as well as the concept of stealing of cell organelles called kleptoplastidy. Moreover, M. rubra represents additional endosymbiosis by transferring its plastids to its predators, the dinoflagellate planktons belonging to the genus Dinophysis. In 2009, a new species of Gram-negative bacteria called Maritalea myrionectae was discovered from a cell culture of M. rubra. M. rubra is a free-living marine ciliate. It is reddish in colour and form dark-red mass during blooming. Its body is almost spherical, looking like a miniature sunflower with its radiating hair-like cilia on its body surface. It measures up to 100 μm in length and 75 μm in width. The body is superficially divided into two lobes due to formation of a constriction at the centre. The constriction gives rise to a larger anterior lobe and a smaller posterior lobe. The cilia arise from the constriction. Using the cilia it can jump about 10-20 times its body length in one movement. Its nucleus is prominently situated at the centre, and is surrounded by organelles mostly derived from algae. For example, its cytoplasm contains numerous plastids, mitochondria and other nuclei. These organelles are properly separated such that the mitochondria are fully enclosed in a vacuole membrane and two endoplasmic reticulum membranes of the ciliate. This indicates that the ciliate is primarily an heterotroph, but after acquiring algal plastid, it transforms into an autotroph. Genetic analysis showed that in the American coastal areas, the primary food of M. rubra is the algae most closely related to the free-living Geminigera cryophila. But in Japanese coasts, the major algal species is Teleaulax amphioxeia. When these plastid-containing algae are ingested by the ciliate, they are not digested. The plastids remain functional and provide nutrition to the ciliate by photosynthesis. In order for the plastids to be normally active, they still require enzymes, which are synthesised by the sequestered algal nuclei. The single nucleus can survive and remain genetically active up to 30 days in the cytoplasm of the ciliate. As the retention time of the prey nuclei is short, an average M. rubra cell may contain eight algal plastids per single prey nucleus and the nuclei need to be replaced by continuous feeding on fresh algae. Thus, the algal organelles are not permanently integrated.