Tobacco streak virus (TSV) is a plant pathogenic virus of the family Bromoviridae, in the genus Ilavirus. It has a wide host range, with at least 200 susceptible species. TSV is generally more problematic in the tropics or warmer climates. TSV does not generally lead to epidemics, with the exception of sunflowers in India and Australia, and peanuts in India. TSV has been reported worldwide in North and South America, Europe, India, Japan, Australia, New Zealand and South America. TSV has a wide host range including both monocots and dicots. Economically important crop hosts include peanuts, sunflower, soybean, cranberry, cotton, chickpea and mung beans. As with many plant viruses, diagnosis is very difficult because TSV has very wide host range and has different effects depending on the host being infected. Symptoms of TSV may include black streaks on stems and leaves, stunted growth, chlorosis, leaf mosaic, lodging, and deformed growing tips, to name a few. On tobacco plants, TSV causes chlorosis, with a unique pattern of white or dark necrotic leaf tissue close to the veins of the leaf. As its name implies, necrotic streaks are found on leaf veins of infected plants. Symptoms are strongly influenced by temperature. For example, plants experiencing a temperature of 20° Celsius will develop small necrotic spots while at temperatures above 30° Celsius, necrotic spots will become large necrotic arcs. Symptoms are not sufficient to correctly identify TSV - serology and PCR techniques are required. TSV purified from infected plants consists of a ss-RNA genome encapsulated by spheroid particles made up of coat protein subunits. The coat protein subunits play a key role in the life cycle of the virus. Once the virus has penetrated into the host cells, it uncoats and releases its viral genomic RNA into the cytoplasm and expressed proteins replicate. After replication, the dsRNA genome is synthesized from genomic ssRNA. Subgenomic RNA4 is translated producing capsid proteins and the new virus particles are assembled. The coat protein of TSV plays an important role in its life cycle. It protects the viral genome and plays a role in cell to cell movement. Once the virus becomes systemic, it can be transmitted by vectors. TSV requires a living plant to survive for a period of time. The virus may be transmitted by thrips vector, mechanical damage, pollen or dodder. Severe outbreaks of TSV typically occur under climatic conditions that allow large thrips populations to develop and when large quantities of pollen are being produced by the host plant. These conditions generally occur in warmer months but are dependent on rainfall and weeds in the field.