In human anatomy, a hamstring (/ˈhæmstrɪŋ/) is one of the three posterior thigh muscles in between the hip and the knee (from medial to lateral: semimembranosus, semitendinosus and biceps femoris). The hamstrings are quite susceptible to injury. In human anatomy, a hamstring (/ˈhæmstrɪŋ/) is one of the three posterior thigh muscles in between the hip and the knee (from medial to lateral: semimembranosus, semitendinosus and biceps femoris). The hamstrings are quite susceptible to injury. In quadrupeds, the hamstring is the single large tendon found behind the knee or comparable area.