Alpha-agarase (EC 3.2.1.158, agarase, agaraseA33) is an enzyme with systematic name agarose 3-glycanohydrolase. This enzyme catalyses the following chemical reaction Alpha-agarase (EC 3.2.1.158, agarase, agaraseA33) is an enzyme with systematic name agarose 3-glycanohydrolase. This enzyme catalyses the following chemical reaction This enzyme requires Ca2+.