Gibson assembly

Gibson assembly® is a molecular cloning method which allows for the joining of multiple DNA fragments in a single, isothermal reaction. It was created by Daniel G. Gibson, Vice President of DNA Technology at Synthetic Genomics Inc (SGI), in collaboration with the J. Craig Venter Institute (JCVI). Gibson assembly® is a molecular cloning method which allows for the joining of multiple DNA fragments in a single, isothermal reaction. It was created by Daniel G. Gibson, Vice President of DNA Technology at Synthetic Genomics Inc (SGI), in collaboration with the J. Craig Venter Institute (JCVI). The entire Gibson assembly reaction requires few components with minor manipulations. The method can simultaneously combine up to 15 DNA fragments based on sequence identity. It requires that the DNA fragments contain ~20-40 base pair overlap with adjacent DNA fragments. These DNA fragments are mixed with a cocktail of three enzymes, along with other buffer components. The three required enzyme activities are: exonuclease, DNA polymerase, and DNA ligase.