Rio Mamore virus (RIOMV) is a novel, single-stranded, enveloped, negative-sense RNA New World orthohantavirus. Rio Mamore virus has been isolated from the pygmy rice rat (Oligoryzomys microtis) in Bolivia. It is genetically related to the Andes virus found in Patagonia in South America. RIOMV has been shown to cause a severe, fatal form of hantavirus pulmonary syndrome (HPS).