Abstract Engineering high‐order multiphoton excited fluorescent (H‐MPEF) materials is of significant importance for sensing, 3D optical data storage and bioimaging. However, it remains a challenging endeavor due to a lack of appropriate construction strategies. This study demonstrates that incorporating octupolar modules within metal‐organic framework (MOF) offers new possibilities for the design of highly attractive H‐MPEF materials. Constructed from a typical MOF ( UiO(bpdc) ), the in situ formation of multibranched octupolar cyclometallated iridium(III) modules via post‐ligand coordination modification endowed the framework with enlarged static hyperpolarizabilities, an extended conjugated system, and enhanced charge transfer, ultimately unlocking its second near‐infrared (NIR‐II, 1000–1700 nm) light activated H‐MPEF performance including three‐ and four‐photon activity. Moreover, these exciting features, combined with subsequent orotic acid‐capping, enabled its application in cancer cell‐specific targeting oncotherapy using tissue‐penetrating NIR‐II light. This finding highlights the vital role of octupoles in H‐MPEF performance and sets a benchmark for unlocking the multiphoton activity of MOFs at the molecular level for deep‐seated tumor fluorescence imaging and therapy.
Cell therapy (also known as cell transplantation) has been considered promising as a next-generation living-cell therapy strategy to surpass the effects of traditional drugs. However, their practical clinical uses and product conversion are hampered by the unsatisfied viability and efficacy of the transplanted cells. Herein, we propose a synergistic enhancement strategy to address these issues by constructing 3D stem cell spheroids integrated with urchin-like hydroxyapatite microparticles (uHA). Specifically, cell-sized uHA microparticles were synthesized
Additive manufacturing (AM), also known as 3D printing, has emerged as a transformative technology in various industries, providing unprecedented design freedom and customized manufacturing solutions. This paper presents a detailed exploration of additive manufacturing applications in metallic materials, introducing the various types of additive manufacturing technologies, elucidating their fundamental principles, and summarizing current research endeavors. The manuscript offers insights into future directions and challenges in this rapidly evolving field, serving as a valuable resource for researchers and engineers aiming to harness the potential of additive manufacturing in advancing metallic materials.
The one-step regulation of planarization and donor rotation provided a convenient method to design theranostic agents for elevating imaging and therapy simultaneously.
Dynamic tracking of the spatiotemporal coordination among various organelles to in-depth understanding of the mechanism of autophagy have attracted considerable attention. However, the monitor of nucleoli participation in autophagy was somehow neglected. Herein, we report a RNA-targeted bioprobe (ADAP) with high selective permeability into nuclear pore complexes, which induced a two-photon (TP) fluorescence "off-on" response by groove combination with RNA, dynamically monitoring the autophagy process among multiorganelles (nucleoli, mitochondria, and mitochondria-containing lysosomes). This work provides a simple and convenient way to observe the dynamic behavior of multiorganelles during the autophagy process, which benefits the understanding of cellular metabolism.
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