Jingliang Su

China Agricultural University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Lijiao Zhang

Institute of Agricultural Resources and Regional Planning

Miro Ferreri

China Agricultural University

Mengxu Sun

Southern Medical University

Bo Han

China Agricultural University

Xueying Hu

Huazhong Agricultural University

Jian Gao

China Agricultural University

Yongyue Wang

Zhejiang Gongshang University

Liben Chen

Johns Hopkins University

Guozhong Zhang

Huazhong Agricultural University

Wenliang Su

China Agricultural University

Cooperative Institutions

China Agricultural University

Chinese Academy of Sciences

Ministry of Agriculture and Rural Affairs

University of Chinese Academy of Sciences

Institute of Microbiology

Chinese Academy of Agricultural Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Wuhan Institute of Virology

Shanghai Jiao Tong University

Central South University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Erratum for Sun et al., “Basic Amino Acid Substitution at Residue 367 of the Envelope Protein of Tembusu Virus Plays a Critical Role in Pathogenesis”

Journal of Virology (2020)

Mengxu Sun Lijiao Zhang Yanxin Cao Jun Wang Ziding Yu

Substitution (logic)

Residue (chemistry)

Envelope (radar)

Pathogenesis

10.1128/jvi.00743-20

Cite

Citations (2)

Isolation and characterization of Streptococcus gallolyticus subsp. pasteurianus causing meningitis in ducklings

Veterinary Microbiology (2012)

Meixia Li Changqin Gu Wanpo Zhang Shuang Li Jiren Liu

Isolation

10.1016/j.vetmic.2012.11.038

Cite

Citations (19)

Antibiotic resistance of Streptococcus agalactiae from cows with mastitis

The Veterinary Journal (2012)

Jian Gao Fuqing Yu Liping Luo Jianzhong He Rong-guang Hou

10.1016/j.tvjl.2012.04.020

Cite

Citations (71)

Long-term passage of duck Tembusu virus in BHK-21 cells generates a completely attenuated and immunogenic population with increased genetic diversity

Vaccine (2019)

Lijiao Zhang Mengxu Sun Qingshui Zhang Jun Wang Yanxin Cao

Flavivirus

Attenuated vaccine

Serial passage

Reverse Genetics

10.1016/j.vaccine.2019.10.080

Cite

Citations (9)

Full Genome Sequences of Two Reticuloendotheliosis Viruses Contaminating Commercial Vaccines

Avian Diseases (2009)

Qinfang Liu Jixun Zhao Jingliang Su Juan Pu Guozhong Zhang

Reticuloendotheliosis virus (REV) fragments are a common contaminant in some commercial vaccines such as fowl poxvirus (FPV) and Marek's disease virus. However, only those strains integrating or containing a near-intact REV provirus are more likely to cause problems in the field. We confirm here, by PCR assays and animal experiments, that vaccines against FPV and herpes virus of turkeys were contaminated with full genome sequences of REV. Further, we determined the complete proviral sequence of two REV isolates from contaminated vaccines. Two REV isolates (REV-99 and REV-06) present in the vaccines were both replication competent, and their proviral genome was 8286 nucleotides in length with two identical long terminal repeats (LTR). The complete genome in these two REV isolates shared 99.8% identity to APC-566 and fowl poxvirus REV proviral inserts (FPV-REV). REV-99 and REV-06 LTR showed over 99% identity to chicken syncytial virus (CSV), but an identity of only 75.8 % and 78.0%, respectively, to SNV. Alignments with other available REV gag, pol, and env sequences revealed high similarity at the nucleotide level. The results further indicated that the prototype CSV may be the most-important REV contaminant in the commercial vaccines, and distinct genotypes of REVs may cocirculate in chicken flocks of China at the present time.

Reticuloendotheliosis virus

Provirus

Flock

10.1637/8579-010609-reg.1

Cite

Citations (31)

Production and Characterization of Monoclonal Antibodies Against Chicken Secretory IgA

Hybridoma (2008)

Yue-Lian Chen Qun Niu Jingliang Su Jixun Zhao Guozhong Zhang

Abstract Two monoclonal antibodies (MAbs) against chicken secretory immunoglobulin A (SIgA) were generated and their binding specificities were characterized by enzyme-linked immunosorbent assay (ELISA), SDS-PAGE, and Western blotting. Analysis revealed that the subtypes of two MAbs were both IgG2b, with the light chain belonging to the kappa configuration. The affinity constant (K(aff)) of the two MAbs was 5.0 x 10(10) M(-1) and 9.7 x 10(9) M(-1), respectively. The MAbs are directed against the heavy chain domains of chicken SigA, and no cross-reactivities to IgG were observed. These results indicate that the MAbs are specific for SIgA and may be a useful tool for investigating issues regarding mucosal immunity and in the development of a good diagnostic kit for detection of specific IgA in chicken.

Secretory IgA

Immunoglobulin A

10.1089/hyb.2008.0026

Cite

Citations (2)

Molecular and Phenotypic Characterization of Aerococcus viridans Associated with Subclinical Bovine Mastitis

PLoS ONE (2015)

Gang Liu Yongxia Liu Tariq Ali Miro Ferreri Jian Gao

Aerococcus viridans is a wide spread bacterium in the environment and clinically this organism is associated with different diseases in animals and humans. However, the geno- and phenotypic characterization of A. viridans associated with bovine mastitis has not yet been reported. The objectives of this study were to investigate the genetic and phenotypic diversity of A. viridans isolates using three different molecular methods including 16S rRNA gene sequencing, pulsed-field gel electrophoresis and random amplified polymorphic DNA (RAPD) along with biochemical tests, including antimicrobial susceptibility test. In total, 60 A. viridans strains were cultured from dairy herds presenting with subclinical mastitis. The results of biochemical tests revealed that most of the isolates (75.0%) were accurately identified by API Rapid 20 Strep system and the majority of A. viridans strains (96.7%) were found to be catalase negative, while two (3.3%) isolates were weakly positive. All isolates were resistant to trimethoprim-sulfamethoxazole, followed by streptomycin (96.7%), tetracycline (65.0%) and clindamycin (56.7%) by minimum inhibition concentration-determining broth microdilution technique. As compared to the sequence of 16S rRNA gene, both PFGE and RAPD showed their capacities to discriminate the intra-species diversity of A. viridans. Furthermore, most of the isolates obtained from the same herd or region belonged to the same major RAPD group, which indicated that RAPD is an appropriate assay for tracking the origins of isolates and epidemiological studies of A. viridans. This is a novel approach to use three molecular techniques and to compare their efficiency regarding the genetic diversity of A. viridans. The data suggest that A. viridans associated with subclinical mastitis has a considerable phenotypic and genotypic diversity.

Viridans streptococci

10.1371/journal.pone.0125001

Cite

Citations (32)

Cephalexin Residue Detection in Milk and Beef by ELISA and Colloidal Gold Based One-Step Strip Assay

Journal of Agricultural and Food Chemistry (2009)

Liben Chen Zhengfang Wang Miro Ferreri Jingliang Su Bo Han

An evaluation of a rapid enzyme-linked immunosorbent assay (ELISA) and colloidal gold based one-step strip assay for cephalexin (CEX) residue detection in milk and beef is described. A monoclonal antibody (mAb) against CEX was produced using cephalexin-bovine serum albumin (CEX-BSA) conjugate as the immunogen, which exhibited no cross-reactivity with applied chemicals in the studied concentration range. The detection limit of rapid ELISA was calculated as 0.39 microg/kg in PBS and 19.5 microg/kg in beef and milk, which was quite lower than the European Union Maximum Residue Limit (MRL) of 100 microg/kg in milk and 200 microg/kg in muscle. Spiked samples were detected with a mean recovery of 82.8-124% and coefficient of variation of 4.88-25%, which indicated a good agreement with the spiked concentration. Accuracy and reproducibility were determined using spiked samples with four different final concentrations of 1, 2, 5, and 10 microg/kg of CEX (n = 7). Mean intra-assay variation of 6.67% and inter-assay variation of 10.66% were obtained. In contrast, the strip test for CEX had a visual detection limit of 0.5 microg/kg, which could be evaluated within 3-10 min. However, positive samples should be further quantified by more sensitive and accurate competitive indirect ELISA method. In conclusion, the described strip test is rapid, simple, and cost-effective as well as sensitive and specific enough for reliable and accurate on-site screening.

Immunogen

Residue (chemistry)

Coefficient of variation

Maximum Residue Limit

Assay sensitivity

Conjugate

10.1021/jf900433d

Cite

Citations (55)

Prototheca zopfii isolated from bovine mastitis induced oxidative stress and apoptosis in bovine mammary epithelial cells

Oncotarget (2017)

Muhammad Shahid Jian Gao Yanan Zhou Gang Liu Tariq Ali

Bovine protothecal mastitis results in considerable economic losses worldwide. However, Prototheca zopfii induced morphological alterations and oxidative stress in bovine mammary epithelial cells (bMECs) is not comprehensively studied yet. Therefore, the aim of this current study was to investigate the P. zopfii induced pathomorphological changes, oxidative stress and apoptosis in bMECs. Oxidative stress was assessed by evaluating catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA) contents and lactate dehydrogenase (LDH) activity, while ROS generation and apoptosis was measured by confocal laser scanning microscopy. The results revealed that infection of P. zopfii genotype II (GTII) significantly changed bMECs morphology, increased apoptotic rate and MDA contents at 12 h (p < 0.05) and 24 h (p < 0.01) in comparison with control group, in time-dependent manner. LDH activity and ROS generation was also increased (p < 0.01) at 12 h and 24 h. However, SOD and CAT contents in bMECs infected with GTII were decreased (p < 0.05) at 12 h, while GPx (p < 0.01), SOD (p < 0.05) and CAT (p < 0.01) levels were reduced at 24 h. In case of GTI, only CAT and GPx activities were significantly decreased when the duration prolonged to 24 h but lesser than GTII. This suggested that GTII has more devastating pathogenic effects in bMECs, and the findings of this study concluded that GTII induced apoptosis and oxidative stress in bMECs via the imbalance of oxidant and antioxidant defenses as well as the production of intracellular ROS.

Malondialdehyde

10.18632/oncotarget.16653

Cite

Citations (23)

Isolation and analysis of two naturally-occurring multi-recombination Newcastle disease viruses in China

Virus Research (2010)

Rui Zhang Xiaoting Wang Jingliang Su Jixun Zhao Guozhong Zhang

Newcastle Disease

Coding region

Lineage (genetic)

10.1016/j.virusres.2010.03.015

Cite

Citations (44)