María Laura Ruiz

Consejo Nacional de Investigaciones Científicas y Técnicas

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Aldo D. Mottino

Consejo Nacional de Investigaciones Científicas y Técnicas

Viviana A. Catania

Instituto de Fisiología Vegetal

Juan Pablo Rigalli

University Hospital Heidelberg

Silvina Stella Maris Villanueva

Consejo Nacional de Investigaciones Científicas y Técnicas

Marcelo G. Luquita

Consejo Nacional de Investigaciones Científicas y Técnicas

Mary Vore

University of Kentucky

Carolina I. Ghanem

University of Buenos Aires

Virginia Perdomo

Consejo Nacional de Investigaciones Científicas y Técnicas

Agostina Arias

Consejo Nacional de Investigaciones Científicas y Técnicas

Enrique J. Sánchez Pozzi

National University of Rosario

Cooperative Institutions

Consejo Nacional de Investigaciones Científicas y Técnicas

National University of Rosario

Instituto de Fisiología Vegetal

Institute of Physics Rosario

University of Buenos Aires

Instituto de Biología Molecular y Celular de Rosario

National University of San Luis

University of Kentucky

Instituto de Cardiología y Cirugía Cardiovascular

Centro Científico Tecnólogico - Rosario

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Biphasic modulation of cAMP levels by the contraceptive nomegestrol acetate. Impact on P-glycoprotein expression and activity in hepatic cells

Biochemical Pharmacology (2018)

Guillermo Nicolás Tocchetti Camila Juliana Domínguez Felipe Zecchinati Maite Rocío Arana María Laura Ruiz

P-glycoprotein

10.1016/j.bcp.2018.04.023

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Citations (3)

Effect of repeated administration with subtoxic doses of acetaminophen to rats on enterohepatic recirculation of a subsequent toxic dose

Biochemical Pharmacology (2009)

Carolina I. Ghanem María Laura Ruiz Silvina Stella Maris Villanueva Marcelo G. Luquita Susana Llesuy

Acetaminophen

Enterohepatic circulation

10.1016/j.bcp.2009.02.006

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Citations (40)

Antibacterial and antioxidant activities of the essential oil of Artemisia echegarayi Hieron. (Asteraceae) Actividad antibacteriana y antioxidante del aceite esencial extraído de Artemisia echegarayi Hieron. (Asteraceae)

DOAJ (DOAJ: Directory of Open Access Journals) (2009)

A. Laciar María Laura Ruiz Roberto Carrizo Flores José R. Saad

Artemisia

Source

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Citations (0)

Neonatal Listeria-meningitis in San Luis, Argentina: a three-case report.

PubMed (2011)

Analía L. Laciar María Laura Ruiz Alban Le Monnier

Neonatal meningitis

10.1590/s0325-75412011000100010

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Citations (6)

HEPATIC AND EXTRAHEPATIC SYNTHESIS AND DISPOSITION OF DINITROPHENYL-S-GLUTATHIONE IN BILE DUCT-LIGATED RATS

Drug Metabolism and Disposition (2006)

Silvina Stella Maris Villanueva María Laura Ruiz Carol J. Soroka Shi‐Ying Cai Marcelo G. Luquita

The ability of the kidney and small intestine to synthesize and subsequently eliminate dinitrophenyl-S-glutathione (DNP-SG), a substrate for the multidrug resistance-associated proteins (Mrps), was assessed in bile duct-ligated (BDL) rats 1, 7, and 14 days after surgery, using an in vivo perfused jejunum model with simultaneous urine collection. A single i.v. dose of 30 μmol/kg b.wt. of 1-chloro-2,4-dinitrobenzene (CDNB) was administered, and its glutathione conjugate DNP-SG and dinitrophenyl cysteinyl glycine derivative, which is the result of γ-glutamyl-transferase action on DNP-SG, were determined in urine and intestinal perfusate by high-performance liquid chromatography. Intestinal excretion of these metabolites was unchanged at day 1, and decreased at days 7 and 14 (–39% and –33%, respectively) after surgery with respect to shams. In contrast, renal excretion was increased by 114%, 150%, and 128% at days 1, 7, and 14. Western blot studies revealed decreased levels of apical Mrp2 in liver and jejunum but increased levels in renal cortex from BDL animals, these changes being maximal between days 7 and 14. Assessment of expression of basolateral Mrp3 at day 14 postsurgery indicated preserved levels in renal cortex, duodenum, jejunum, distal ileum, and colon. Analysis of expression of glutathione-S-transferases α, μ, and π, as well as activity toward CDNB, indicates that formation of DNP-SG was impaired in liver, preserved in intestine, and increased in renal cortex. In conclusion, increased renal tubular conversion of CDNB to DNP-SG followed by subsequent Mrp2-mediated secretion into urine partially compensates for altered liver function in experimental obstructive cholestasis.

Dinitrophenyl

Disposition

10.1124/dmd.106.009415

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Citations (19)

ETHYNYLESTRADIOL INCREASES EXPRESSION AND ACTIVITY OF RAT LIVER MRP3

Drug Metabolism and Disposition (2006)

María Laura Ruiz Silvina Stella Maris Villanueva Marcelo G. Luquita Mary Vore Aldo D. Mottino

We evaluated the effect of ethynylestradiol (EE) administration (5 mg/kg b.wt. s.c., for 5 consecutive days) on the expression and activity of multidrug resistance-associated protein 3 (Mrp3) in rats. Western blotting analysis revealed decreased Mrp2 (-41%) and increased Mrp3 (+200%) expression by EE. To determine the functional impact of up-regulation of Mrp3 versus Mrp2, we measured the excretion of acetaminophen glucuronide (APAP-glu), a common substrate for both transporters, into bile and perfusate in the recirculating isolated perfused liver (IPL) model. APAP-glu was generated endogenously from acetaminophen (APAP), which was administered as a tracer dose (2 μmol/ml) into the perfusate. Biliary excretion of APAP-glu after 60 min of perfusion was reduced in EE-treated rats (-80%). In contrast, excretion into the perfusate was increased by EE (+45%). Liver content of APAP-glu at the end of the experiment was reduced by 36% in the EE group. The total amount of glucuronide remained the same in both groups. Taken together, these results indicate that up-regulation of Mrp3 led to an exacerbated basolateral versus canalicular excretion of conjugated APAP in IPL. We conclude that induced expression of basolateral Mrp3 by EE may represent a compensatory mechanism to prevent intracellular accumulation of common Mrp substrates, either endogenous or exogenous, due to reduced expression and activity of apical Mrp2.

10.1124/dmd.106.009316

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Citations (25)

Antibacterial and antioxidant activities of the essential oil of Artemisia echegarayi Hieron. (Asteraceae).

PubMed (2010)

A. Laciar María Laura Ruiz Roberto Carrizo Flores José Roberto Cury Saad

Camphor

Artemisia

Terpene

Borneol

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Citations (39)

Pregnane X receptor mediates the induction of P-glycoprotein by spironolactone in HepG2 cells

Toxicology (2011)

Juan Pablo Rigalli María Laura Ruiz Virginia Perdomo Silvina Stella Maris Villanueva Aldo D. Mottino

Pregnane X receptor

P-glycoprotein

Constitutive androstane receptor

Rhodamine 123

10.1016/j.tox.2011.03.015

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Citations (49)

Involvement of Mrp2 in Hepatic and Intestinal Disposition of Dinitrophenyl-S-glutathione in Partially Hepatectomized Rats

Toxicological Sciences (2004)

Silvina Stella Maris Villanueva María Laura Ruiz Marcelo G. Luquita Enrique J. Sánchez Pozzi Viviana A. Catania

The ability of the liver and small intestine for secretion of dinitrophenyl-S-glutathione (DNP-SG), a substrate for multidrug resistance-associated protein 2 (Mrp2), into bile and lumen, respectively, as well as expression of Mrp2 in both tissues, were assessed in 70–75% hepatectomized rats. An in vivo perfused intestinal model was used. A single iv dose of 30 μmol/kg b.w. of 1-chloro-2,4-dinitrobenzene (CDNB) was administered and its glutathione conjugate, DNP-SG, was determined by HPLC in bile and intestinal perfusate. One and seven days after hepatectomy, biliary excretion of DNP-SG was decreased by 90 and 50% with respect to shams, respectively, when expressed per mass unit. In contrast, intestinal excretion was increased by 63% or unchanged one and seven days post-hepatectomy, respectively. Tissue content of DNP-SG 5 min after CDNB administration was substantially decreased in liver and significantly increased in intestine, one day post-hepatectomy. Western and immunofluorescence studies revealed preserved levels and localization of Mrp2 in both tissues from hepatectomized animals, irrespective of the time analyzed. In spite of preserved expression of Mrp2, the higher availability of DNP-SG in intestinal cells, likely as a consequence of increased glutathione-S-transferase-mediated conjugation of CDNB, may explain the in vivo findings. Further experiments in isolated hepatocytes suggested that decreased synthesis of DNP-SG rather than altered canalicular transport is responsible for the substantial impairment in excretion of this compound into bile. Taken together, these results indicate that the intestine may partially compensate for liver DNP-SG disposition, particularly shortly after surgery, while liver capability is recovering.

Dinitrophenyl

10.1093/toxsci/kfi053

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Citations (13)

Expression and function of Oat1 and Oat3 in rat kidney exposed to mercuric chloride

Archives of Toxicology (2009)

Gisela Di Giusto Naohiko Anzai María Laura Ruiz Hitoshi Endou Adriana M. Torres

10.1007/s00204-009-0445-8

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Citations (29)