Shitao Hu

Hunan Agricultural University

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Qiaolin Liu

National Center for Nanoscience and Technology

Baohong Xu

Hunan Agricultural University

Le Yang

Beijing Technology and Business University

Haitai Chen

Hunan Agricultural University

Tiaoyi Xiao

Hunan Agricultural University

Xiangbei Tang

Hunan Agricultural University

Chong Wang

Chongqing Medical University

Le Yang

Fujian Medical University

Yuhong Huang

Hunan Agricultural University

Shuting Xiong

Hunan Agricultural University

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Hunan Agricultural University

Yueyang Changling Equipment Research Institute (China)

Anhui Agricultural University

Huazhong University of Science and Technology

Beijing Technology and Business University

Key Laboratory of Guangdong Province

Shandong Agricultural University

Industrial Technology Research Institute

National Center for Nanoscience and Technology

General Hospital of Guangzhou Military Command

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Gonadal Development and Differentiation of Hybrid F1 Line of Ctenopharyngodon idella (♀) × Squaliobarbus curriculus (♂)

International Journal of Molecular Sciences (2024)

Qiaolin Liu Shitao Hu Xiangbei Tang Chong Wang Le Yang

The hybrid F1 offspring of Ctenopharyngodon idella (♂) and Squaliobarbus curriculus (♀) exhibit heterosis in disease resistance and also show abnormal sex differentiation. To understand the mechanism behind gonadal differentiation in the hybrid F1, we analyzed the transcriptomes of C. idella, S. curriculus, and the hybrid F1; screened for genes related to gonad development in these samples; and measured their expression levels. Our results revealed that compared to either C. idella or S. curriculus, the gene expressions in most sub-pathways of the SNARE interactions in the vesicular transport pathway in the hypothalamus, pituitary, and gonadal tissues of their hybrid F1 offspring were significantly up-regulated. Furthermore, insufficient transcription of genes involved in oocyte meiosis may be the main reason for the insufficient reproductive ability of the hybrid F1 offspring. Through transcriptome screening, we identified key molecules involved in gonad development, including HSD3B7, HSD17B1, HSD17B3, HSD20B2, CYP17A2, CYP1B1, CYP2AA12, UGT2A1, UGT1A1, and FSHR, which showed significant differences in expression levels in the hypothalamus, pituitary, and gonads of these fish. Notably, the expression levels of UGT1A1 in the gonads of the hybrid F1 were significantly higher than those in C. idella and S. curriculus. These results provide a scientific basis for further research on the gonadal differentiation mechanism of hybrid F1 offspring.

10.3390/ijms251910566

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Genome-wide identification of Gα family in grass carp (Ctenopharyngodon idella) and reproductive regulation functional characteristics of Cignaq

BMC Genomics (2024)

Chong Wang Shuting Xiong Shitao Hu Le Yang Yuhong Huang

The Gα family plays a crucial role in the complex reproductive regulatory network of teleosts. However, the characterization and function of Gα family members, especially Gαq, remain poorly understood in teleosts. To analyze the characterization, expression, and function of grass carp (Ctenopharyngodon idella) Gαq, we identified the Gα family members in grass carp genome, and analyzed the expression, distribution, and signal transduction of Gαq/gnaq. We also explored the role of Gαq in the reproductive regulation of grass carp. Our results showed that the grass carp genome contains 27 Gα genes with 46 isoforms, which are divided into four subfamilies: Gαs, Gαi/o, Gαq/11, and Gα12/13. The expression level of Cignaq in the testis was the highest and significantly higher than in other tissues, followed by the hypothalamus and brain. The luteinizing hormone receptor (LHR) was mainly localized to the nucleus in grass carp oocytes, with signals also present in follicular cells. In contrast, Gαq signal was mainly found in the cytoplasm of oocytes, with no signal in follicular cells. In the testis, Gαq and LHR were co-localized in the cytoplasm. Furthermore, the grass carp Gαq recombinant protein significantly promoted Cipgr expression. These results provided preliminary evidence for understanding the role of Gαq in the reproductive regulation of teleosts.

Grass carp

10.1186/s12864-024-10717-0

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