Rohit Singh

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Swagatika Priyadarsini

Indian Veterinary Research Institute

María Peña-Chilet

Fundación Progreso y Salud

Leonor Saiz

University of California, Davis

Edward S.C. Shih

Institute of Biomedical Sciences, Academia Sinica

Bernd Bodenmiller

ETH Zurich

Marco Tognetti

Biognosys (Switzerland)

Jie Wang

Yangzhou University

Godfred Yawson Scott

Kwame Nkrumah University of Science and Technology

Nehal Sharma

Jiajie Peng

Northwestern Polytechnical University

Cooperative Institutions

University Hospital Heidelberg

Heidelberg University

Fundación Progreso y Salud

University of Luxembourg

Harvard University

Indraprastha Institute of Information Technology Delhi

Indian Institute of Technology Delhi

Centre for Biomedical Network Research on Rare Diseases

Instituto de Biomedicina de Sevilla

Consejería de Salud y Consumo

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

SMART WINDMILL and WIND SOLAR TREE

Authorea (Authorea) (2024)

Kamlesh Singh Rohit Singh Prateek Singh Brijbhan Singh

* The present invention relates to the harnessing of green and renewable energy. And various methods to maximize such energy generation efforts in a micro power plant model. Also explores the description of a smart windmill, for green energy generation purposes, combined with Solar panels.

Windmill

Solar Power

10.22541/au.170664204.43767853/v1

Cite

Citations (0)

Real Time PCR and Its Application in Diagnosis of Current Veterinary Diseases: A Brief Review

International Journal of Current Microbiology and Applied Sciences (2019)

Rohit Singh Swagatika Priyadarsini Preeti Singh Somesh Joshi

Diagnosis of disease is the backbone of control and treatment in veterinary field. In addition to the antemortem and post mortem methods, currently several laboratory-based tools and technique are also being used for early diagnosis. Since few decades, polymerase chain reaction (PCR) has emerged as the most preferred molecular diagnostic technique for disease diagnosis due to its high specificity. But it only detects the presence of the target nucleic acid in the sample without quantifying the same. Additionally, the detection of amplified DNA requires one extra step of gel electrophoresis followed by visualization under ultraviolet rays which involves radiation hazards. Hence, a more sophisticated technique called real time polymerase chain reaction (PCR) has been discovered for developing rapid assay for the diagnosis of many diseases. Along with the detection of particular nucleotide sequence, quantification of the latter can also be performed using this assay. Real time PCR was either of two specific chemistry: Nonspecific DNA binding dye or specific hybridization probe. The fluorescence generated from either of the above during the assay is directly proportional to the quantity of target being amplified at the real time. Although field application of real time PCR is infrequent, nevertheless its rapidity, high sensitivity & specificity and less contamination risk may lead to its enhanced application in screening and epidemiological study in the veterinary field in recent future. In this review we attempted to brief about the chemistries of real time PCR and its application in diagnosis of different veterinary diseases worldwide.

10.20546/ijcmas.2019.810.275

Cite

Citations (1)

Cell‐to‐cell and type‐to‐type heterogeneity of signaling networks: insights from the crowd

Molecular Systems Biology (2021)

Attila Gábor Marco Tognetti Alice Driessen Jovan Tanevski Baosen Guo

Recent technological developments allow us to measure the status of dozens of proteins in individual cells. This opens the way to understand the heterogeneity of complex multi-signaling networks across cells and cell types, with important implications to understand and treat diseases such as cancer. These technologies are, however, limited to proteins for which antibodies are available and are fairly costly, making predictions of new markers and of existing markers under new conditions a valuable alternative. To assess our capacity to make such predictions and boost further methodological development, we organized the Single Cell Signaling in Breast Cancer DREAM challenge. We used a mass cytometry dataset, covering 36 markers in over 4,000 conditions totaling 80 million single cells across 67 breast cancer cell lines. Through four increasingly difficult subchallenges, the participants predicted missing markers, new conditions, and the time-course response of single cells to stimuli in the presence and absence of kinase inhibitors. The challenge results show that despite the stochastic nature of signal transduction in single cells, the signaling events are tightly controlled and machine learning methods can accurately predict new experimental data.

Mass cytometry

Cell type

Single-Cell Analysis

10.15252/msb.202110402

Cite

Citations (12)

Unveiling the Global Surge of Mpox (Monkeypox): A comprehensive review of current evidence

The Microbe (2024)

Phelipe Magalhães Duarte Ridwan Olamilekan Adesola Swagatika Priyadarsini Rohit Singh Mohamed N. F. Shaheen

Monkeypox, now named mpox, has emerged as a significant public threat, as evidenced by the 2022 outbreak affecting over seventy countries globally. This infectious disease presents debilitating symptoms, including painful skin rashes, mucosal lesions, enlarged lymph nodes, and fever. The situation intensifies with concerns about a novel transmission route, specifically through sexual contact, and the evolution of more transmissible strains. Complicating matters further is the documented spillback of mpox from humans to animals, raising the potential for new animal reservoirs. This study utilized a systematic approach to gather, analyse, and interpret data regarding global mpox outbreaks, phylogenomics, human APOBEC3 enzyme activity, antiviral resistance issues, and the application of the One Health intervention. Emphasizing a One Health approach covers various aspects, including zoonotic origins, pathogenesis, changing epidemiological landscapes, phylogenomic diversity, and clade dynamics. The review underscores the crucial role of global collaboration in understanding and combatting mpox and making it a valuable resource for shaping effective prevention and control measures on a global scale.

Monkeypox

10.1016/j.microb.2024.100141

Cite

Citations (10)