Wu-Chen Guo

University of Science and Technology of China

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Dong‐Liang Huang

University of Science and Technology of China

Lei Liu

University of Science and Technology of China

Yongkang Zhou

University of Science and Technology of China

Ji‐Shen Zheng

University of Science and Technology of China

Long-Jie Wang

University of Science and Technology of China

Wei-Wei Shi

Center for Life Sciences

Shan Tang

University of Science and Technology of China

Yun-Pu Gao

University of Science and Technology of China

Chen Wang

University of Utah

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University of Science and Technology of China

Center for Life Sciences

Tsinghua University

Hefei National Center for Physical Sciences at Nanoscale

Chinese Academy of Sciences

High Magnetic Field Laboratory

Yantai Institute of Coastal Zone Research

Anhui University

Nanjing University

Collaborative Innovation Center of Advanced Microstructures

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Enhanced native chemical ligation by peptide conjugation in trifluoroacetic acid

Science Advances (2024)

Dong‐Liang Huang Wu-Chen Guo Wei-Wei Shi Yun-Pu Gao Yongkang Zhou

Chemical ligation of peptides is increasingly used to generate proteins not readily accessible by recombinant approaches. However, a robust method to ligate "difficult" peptides remains to be developed. Here, we report an enhanced native chemical ligation strategy mediated by peptide conjugation in trifluoroacetic acid (TFA). The conjugation between a carboxyl-terminal peptide thiosalicylaldehyde thioester and a 1,3-dithiol-containing peptide in TFA proceeds rapidly to form a thioacetal-linked intermediate, which is readily converted into the desired native amide bond product through simple postligation treatment. The effectiveness and practicality of the method was demonstrated by the successful synthesis of several challenging proteins, including the SARS-CoV-2 transmembrane Envelope (E) protein and nanobodies. Because of the ability of TFA to dissolve virtually all peptides and prevent the formation of unreactive peptide structures, the method is expected to open new opportunities for synthesizing all families of proteins, particularly those with aggregable or colloidal peptide segments.

Native chemical ligation

Trifluoroacetic acid

Chemical Ligation

Thioester

Peptide bond

10.1126/sciadv.ado9413

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