Biyun Liao

Youjiang Medical College for Nationalities

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Yuxia Wei

Karolinska Institutet

Haimei Qin

Affiliated Hospital of Youjiang Medical University for Nationalities

Linlin Hu

Affiliated Hospital of Youjiang Medical University for Nationalities

Xiaoxia Pang

Affiliated Hospital of Youjiang Medical University for Nationalities

Fenglian Yang

Youjiang Medical College for Nationalities

Guijiang Wei

Affiliated Hospital of Youjiang Medical University for Nationalities

Junli Wang

South China University of Technology

Junli Wang

Affiliated Hospital of Youjiang Medical University for Nationalities

Feng‐Lian Yang

Huazhong Agricultural University

Xiaocan Lei

University of South China

Cooperative Institutions

Affiliated Hospital of Youjiang Medical University for Nationalities

University of South China

Sun Yat-sen University

Youjiang Medical College for Nationalities

Anhui Medical University

Capital Medical University

Nanjing Agricultural University

Central South University

Ministry of Agriculture and Rural Affairs

Chinese Academy of Agricultural Sciences

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LncRNA HOTAIR regulates the expression of E-cadherin to affect nasopharyngeal carcinoma progression by recruiting histone methylase EZH2 to mediate H3K27 trimethylation

Research Square (Research Square) (2019)

Fenglian Yang Yuxia Wei Biyun Liao Guijiang Wei Haimei Qin

Abstract Background In recent years, there has been increasing evidence for the function of long non-coding RNA (lncRNA) in nasopharyngeal carcinoma (NPC).. We aim to delve into the position of lncRNA HOTAIR, together with EZH2, E-cadherin and H3K27me3 in NPC and explore the related mechanisms. Methods RT-qPCR and western blot analysis were carried out for detecting lncRNA HOTAIR, EZH2, E-cadherin and H3K27me3 expression in NPC tissues and cells. Moreover, the correlations between lncRNA HOTAIR and EZH2 expression and the clinicopathological characteristics and prognosis of patients with NPC were observed. NPC cell biological functions were examined through gain-of and loss-of function assays. RIP and ChIP assays were applied to detect whether lncRNA HOTAIR in NPC cells could regulate E-cadherin by recruiting EZH2 to mediate trimethylation of H3K27. Results LncRNA HOTAIR, EZH2, and H3K27me3 were richly expressed in NPC tissues and cells, and E-cadherin was lowly expressed. The prognosis of patients with overexpression of lncRNA HOTAIR and EZH2 was worse than that of patients with theirs low expression. Down-regulation of either HOTAIR or EZH2 inhibited cell proliferation, promoted apoptosis, suppressed migration and invasion and inhibited tumor growth. HOTAIR recruited histone methylase EZH2 to mediate trimethylation of H3K27 and regulated E-cadherin expression. Conclusion Our study suggests that lncRNA HOTAIR inhibits the expression of E-cadherin by stimulating the trimethylation of H3K27 by histone methylase EZH2 to promote cell migration, proliferation, and inhibit apoptosis of NPC cells.

HOTAIR

PRC2

10.21203/rs.2.17457/v1

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Effects of vascular endothelial growth factor on in vitro fertilization of buffalo oocytes.

Li Jiang Hongli Pan Li MinLing Biyun Liao Qiang Fu

【Objective】The present study was designed to investigate the effects of vascular endothelial growth factor(VEGF) on in vitro fertilization of buffalo oocytes in order to improve its culture system and the efficiency of embryo production.【Method】Using cumulus cell-oocyte compound material(COCs) with over three layers entirely enclosed by cumulus cells as test materials,the effects of VEGF were investigated on buffalo oocytes in vitro maturation,fertilization and early embryo development by supplementation of recombinant human VEGF165 in maturation and fertilization culture medium.【Result】With 10 ng/mL VEGF adding into the maturation medium,the rate of the first polar body extrusion after oocyte maturation was increased significantly(from 57.3% to 72.3%,P0.05),and the cleavage rate of zygotes was also increased significantly(from 41.0% to 53.3%,P0.05).Supplementation of fertilization medium with 10 ng/mL VEGF increased the cleavage rate of zygotes highly significantly(from 41.9% to 63.0%,P0.01),and increased the blastocyst development rate significantly(from 17.0% to 32.6%,P0.05).When both maturation and fertilization media were supplemented with 10 ng/mL VEGF,both the cleavage and blastocyst rates were highly significantly increased(from 43.4% to 64.3%,19.0% to 27.2%; P0.01),and the blastocyst cell number was significantly higher than the control(from 89.5 to 106.8,P0.05).【Conclusion】VEGF had positive effects on buffalo oocyte in vitro maturation and fertilization,and early embryo development.Therefore,it could be used to optimize the culture system for buffalo in vitro fertilization and improve its in vitro embryo production efficiency.

In vitro maturation

Cleavage (geology)

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Effects of Sleep Disorders and Circadian Rhythm Changes on Male Reproductive Health: A Systematic Review and Meta-analysis

Frontiers in Physiology (2022)

Ou Zhong Biyun Liao Jinyuan Wang Ke Liu Xiaocan Lei

Objectives: The purpose of this study was to elucidate the relationship between sleep disorders and male reproductive health, and to explore the underlying mechanisms via a systematic review and meta-analysis. Methods: PubMed, Embase, The Cochrane library, Web of Science, Scopus databases were searched to collect clinical research on the effects of sleep disorders on male semen parameters from inception to February 24, 2022. RevMan 5.4 was used for meta-statistical analysis. Stata16 software was used to detect publication bias. Results: The results of meta-analysis showed that sleep disorders were associated with reduced total sperm count (mean difference (MD) = -27.91, 95% CI = (-37.82, -18.01), p < 0.001), reduced sperm concentration (MD = -5.16, 95% CI = (-9.67, -0.65), p = 0.02), reduced progressive motility (MD = -2.94, 95% CI = (-5.28, -0.59), p = 0.01), and reduced normal morphology (MD = -0.52, 95% CI = (-0.80, -0.24), p < 0.001). However, there is no significant association between sleep disorders and semen volume/reproductive hormones. Further bioinformatics mining revealed that related clock genes (PER1, PER2, CRY2, NR1D1 and NPAS2) were down-regulated in non-obstructive azoospermia patients. Conclusion: In conclusion, current evidence suggests that sleep disorders have a negative impact on male reproductive health, and its underlying mechanism may be related to circadian rhythm disorders. However, the relationship between sleep disorders and reproductive hormone levels has not been found. Due to the limited number and quality of included studies, the above findings need to be validated by more high-quality studies.

Semen quality

Sleep

10.3389/fphys.2022.913369

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Citations (8)

Exposure to nonylphenol impairs oocyte quality via the induction of organelle defects in mice

Ecotoxicology and Environmental Safety (2022)

Linlin Hu Hongge Li Biyun Liao Yi Xu Shao‐Chen Sun

Nonylphenol (NP) is an environmental endocrine disruptor, which is mainly used in the production of surfactants, lubricants, additives, pesticides, and emulsifiers. NP is widely found in sewage and sludge, which has neurotoxicity, immunotoxicity, metabolic toxicity and reproductive toxicity. In this study, we investigated the effects of NP exposure on mammalian oocyte quality from organelle aspects with mouse in vivo model. The results showed that the ovarian weight of mice exposed to 500 μg/L NP for 4 weeks increased and the development ability of oocytes decreased, showing with lower rate of polar body extrusion. Further analysis indicated that exposure to NP caused the abnormal distribution of mitochondria, following with altered membrane potential drop. NP exposure disrupted the spindle periphery localization of ER, and affected the expression of GRP78 for the induction of ER stress. Moreover, Golgi apparatus fragment in the oocytes was observed, and Rab11-based vesicle transport was disturbed. We also found that the protein degradation might be affected since LAMP2 expression increased and LC3 decreased, indicating the lysosome and autophagy dysfunction. Taken together, our findings suggested that the exposure of NP to mice in vivo affected oocyte quality through its effects on the distribution and function of organelles.

Nonylphenol

Organelle

Endocrine disruptor

Germinal vesicle

Reproductive toxicity

10.1016/j.ecoenv.2021.113136

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Citations (11)

HT-2 toxin impairs porcine oocyte in vitro maturation through disruption of endomembrane system

Theriogenology (2024)

Jiarui Li Si-Le Wu Linlin Hu Biyun Liao Shao‐Chen Sun

Brefeldin A

Nocodazole

Endomembrane system

COPI

10.1016/j.theriogenology.2024.06.019

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Citations (2)

Uncovering the ceRNA network and DNA methylation associated with gene expression in nasopharyngeal carcinoma

BMC Medical Genomics (2023)

Ting Zhang Pei Lu Wenli Qiu Yuxia Wei Biyun Liao

Abstract Objective This study aimed to uncover abnormally expressed genes regulated by competitive endogenous RNA (ceRNA) and DNA methylation nasopharyngeal carcinoma and to validate the role of lncRNAs in the ceRNA network on nasopharyngeal carcinoma progression. Methods Based on the GSE64634 (mRNA), GSE32960 (miRNA), GSE95166 (lncRNA), and GSE126683 (lncRNA) datasets, we screened differentially expressed mRNAs, miRNAs and lncRNAs in nasopharyngeal carcinoma. A ceRNA network was subsequently constructed. Differentially methylated genes were screened using the GSE62336 dataset. The abnormally expressed genes regulated by both the ceRNA network and DNA methylation were identified. In the ceRNA network, the expression of RP11-545G3.1 lncRNA was validated in nasopharyngeal carcinoma tissues and cells by RT-qPCR. After a knockdown of RP11-545G3.1, the viability, migration, and invasion of CNE-2 and NP69 cells was assessed by CCK-8, wound healing and Transwell assays. Results This study identified abnormally expressed mRNAs, miRNAs and lncRNAs in nasopharyngeal carcinoma tissues. A ceRNA network was constructed, which contained three lncRNAs, 15 miRNAs and 129 mRNAs. Among the nodes in the PPI network based on the mRNAs in the ceRNA network, HMGA1 was assessed in relation to the overall and disease-free survival of nasopharyngeal carcinoma. We screened two up-regulated genes regulated by the ceRNA network and hypomethylation and 26 down-regulated genes regulated by the ceRNA network and hypermethylation. RP11-545G3.1 was highly expressed in the nasopharyngeal carcinoma tissues and cells. Moreover, the knockdown of RP11-545G3.1 reduced the viability, migration, and invasion of CNE-2 and NP69 cells. Conclusion Our findings uncovered the epigenetic regulation in nasopharyngeal carcinoma and identified the implications of RP11-545G3.1 on the progression of nasopharyngeal carcinoma.

Competing Endogenous RNA

10.1186/s12920-023-01653-1

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Effects of Lycium barbarum Polysaccharide on Endoplasmic Reticulum Stress and Oxidative Stress in Obese Mice

Frontiers in Pharmacology (2020)

Fenglian Yang Yuxia Wei Biyun Liao Guijiang Wei Haimei Qin

Background: The incidence of obesity-associated decline in male fertility has increased over the years. Lycium barbarum polysaccharide (LBP), a natural plant polysaccharide extracted from the Chinese herb L. barbarum has shown promising therapeutic effects in overcoming the same. Aim: This study aimed to investigate the protective effect of LBP on the testes of obese mice. Methods: Following administration of LBP to high-fat diet-induced obese mice for 35 days, serum, sperm, and testis samples were obtained for subsequent experiments. Biochemical analysis and sex hormone content determination were performed to observe changes in glycolipid metabolism and testosterone levels, respectively, in the blood. Hematoxylin and eosin staining were carried out to assess the pathological changes in the testicular tissue. Oxidative stress levels were detected using enzyme-linked immunosorbent assay and expression levels of endoplasmic reticulum stress markers were determined using western blot in the testicular tissue. Results: Our results suggested that LBP reduced glucose levels and insulin resistance, increased testosterone levels and insulin sensitivity, and decreased testicular oxidative stress and pathological damage in obese mice. In addition, LBP down-regulated the expression of p-eIF2α, GRP78, and CHOP in the testicular tissues of obese mice. Conclusion: Our results shows that LBP is a potential novel drug for preventing male infertility caused by obesity.

Lycium

10.3389/fphar.2020.00742

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Citations (24)

Bisphenol A Exposure Disrupts Organelle Distribution and Functions During Mouse Oocyte Maturation

Frontiers in Cell and Developmental Biology (2021)

Meng‐Hao Pan Yuke Wu Biyun Liao Hui Zhang Chan Li

Bisphenol A (BPA) is one of the ubiquitous environmental endocrine disruptors (EEDs). Previous studies have shown that the reproduction toxicity of BPA could cause severe effects on the mammal oocytes and disturb the quality of mature oocytes. However, the toxic effects of BPA on the organelles of mouse oocytes have not been reported. In this study, to investigate whether BPA can be toxic to the organelles, we used different concentrations of BPA (50, 100, and 200 μM) to culture mouse oocytes in vitro . The results showed that 100 μM BPA exposure could significantly decrease the developmental capacity of oocytes. Then, we used the immunofluorescence staining, confocal microscopy, and western blotting to investigate the toxic effects of BPA on the organelles. The results revealed that mitochondrial dysfunction is manifested by abnormal distribution and decreased mitochondrial membrane potential. Moreover, the endoplasmic reticulum (ER) is abnormally distributed which is accompanied by ER stress showing increased expression of GRP78. For the Golgi apparatus, BPA-exposed dose not disorder the Golgi apparatus distribution but caused abnormal structure of Golgi apparatus, which is manifested by the decrease of GM130 protein expression. Moreover, we also found that BPA-exposed led to the damage of lysosome, which were shown by the increase of LAMP2 protein expression. Collectively, our findings demonstrated that the exposure of BPA could damage the normal function of the organelles, which may explain the reduced maturation quality of oocytes.

Organelle

Endocrine disruptor

10.3389/fcell.2021.661155

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Citations (32)

RETRACTED: LncRNA HOTAIR regulates the expression of E-cadherin to affect nasopharyngeal carcinoma progression by recruiting histone methylase EZH2 to mediate H3K27 trimethylation

Genomics (2021)

Feng‐Lian Yang Yuxia Wei Biyun Liao Guijiang Wei Haimei Qin

HOTAIR

PRC2

10.1016/j.ygeno.2021.03.036

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