In April 2020, KAGRA conducted its first science observation in combination with the GEO~600 detector (O3GK) for two weeks. According to the noise budget estimation, suspension control noise in the low frequency band and acoustic noise in the middle frequency band are identified as the dominant contribution. In this study, we show that such noise can be reduced in offline data analysis by utilizing a method called Independent Component Analysis (ICA). Here the ICA model is extended from the one studied in iKAGRA data analysis by incorporating frequency dependence while linearity and stationarity of the couplings are still assumed. By using optimal witness sensors, those two dominant contributions are mitigated in the real observational data. We also analyze the stability of the transfer functions for whole two weeks data in order to investigate how the current subtraction method can be practically used in gravitational wave search.

Subtraction

Component (thermodynamics)

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Purification of human haptoglobin 1-1, 2-1, and 2-2 using monoclonal antibody affinity chromatography

The FASEB Journal (2004)

CF Tseng H-Y. Huang YT Yang Sjt Mao

Similar to blood type, human plasma haptoglobin (Hp) is classified as 3 phenotypes: Hp 1-1, 2-1, or 2-2. The structural and functional relationship between the phenotypes, however, has not been studied in detail due to the complicated and difficult isolation procedures. This report provides a simple protocol that can be used to purify each Hp phenotype. Plasma was first passed through an affinity column coupled with a high affinity Hp monoclonal antibody. The bound material was washed with a buffer containing 0.2M NaCl and 0.02 M phosphate, pH 7.4, eluted at pH 11, and collected in tubes containing 1M Tris-HCl, pH 6.8. The crude Hp fraction was then chromatographed on a HPLC Superose 12 column in 0.05 M ammonium bicarbonate at a flow rate of 0.5 ml/min. The homogeneity of purified Hp 1-1, 2-1, or 2-2 was greater than 95% as judged by SDS-polyacrylamide gel electrophoresis. Essentially, each Hp isolated was not contaminated with hemoglobin and apolipoprotein A-I as that reported from the other methods, and was able to bind hemoglobin. Neuraminidase treatment demonstrated that the purified Hp possessed a carbohydrate moiety, while Western blot analysis confirmed alpha and beta chains corresponding to each Hp 1-1, 2-1, and 2-2 phenotype. The procedures described here represent a significant improvement in current purification methods for the isolation of Hp phenotypes. Circular dichroic spectra showed that the alpha-helical content of Hp 1-1 (29%) was higher than that of Hp 2-1 (22%), and 2-2 (21%). The structural difference with respect to its clinical relevance is discussed.

Haptoglobin

Sephadex

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Additional new 5,6-dihydroflavanones and cytotoxic constituents from the leaves of Cryptocarya chinensis

Planta Medica (2009)

TH Chou Jianjun Chen SJ Lee H-Y. Huang IS Chen

Cryptocarya chinensis (Hance) Hemsl. (Lauraceae) is a medium-sized evergreen tree, distributed in southern China, Japan, and Taiwan [1]. Approximately 1400 species of Formosan plants have been screened for cytotoxicity, and C. chinensis was shown to be one of the active species. Pavine alkaloids and their derivatives have been extensively studied from the basic fraction of this species [2–6]. However, the neutral-CHCl3 soluble fraction of this plant has not been studied. Investigation of the neutral-CHCl3 soluble fraction of the leaves of C. chinensis has led to the isolation of additional four new 5,6-dihydroflavanone skeleton, cryptochinenone A (1), B (2), C (3) and D (4), together with ten known compounds. Among the all isolates, infectocaryone, crytocaryone, 4'-dihydroxy-2',6'-dimethoxychalcone, cryptocaryanone A and B showed cytotoxic activities (≤ 7.25 µg/mL) against MCF-7, NCI-H460 and SF-268 cell lines, respectively. The structures of these new compounds were determined through spectroscopic analyses including extensive 2D-NMR, X-ray, and CD-ORD data. This poster describes the structural elucidation of these new compounds and the cytotoxic activities.

Lauraceae

Fraction (chemistry)

10.1055/s-0029-1234640

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A proteomic study of peripheral blood mononuclear cells in systemic lupus erythematosus

Lupus (2008)

Yong Dai Chaofan Hu Yi Huang H-Y. Huang J Liu

Our objective was to analyze the changes in the protein expression profiles of peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE). Peripheral blood was obtained from patients with SLE and healthy controls. 2-D gel electrophoresis was performed, and gels were silver-stained. Differentially expressed protein spots were detected, some of which were identified by MALDI-TOF spectrometry. Match rates of 71% ± 4% and 72% ± 4% were gotten for control and patient gels, respectively. 791 ± 17 spots were detected for control gels and 781 ± 17 for patient gels. Eleven protein spots were up-regulated, and 9 protein spots were down-regulated in patients with SLE. Five differentially expressed proteins were identified as immunoglobulin J chain, apolipoprotein A-IV precursor, calprotectin L1H and zinc finger protein subfamily 1A (all up-regulated) and glutathione S-transferase (down-regulated), some of which had previously been shown to play a potential role in the pathogenesis of SLE. We conclude there are significant changes in the 2-D maps of PBMCs in patients with SLE and applying this proteomic approach may be a useful way to gain novel insights into SLE.

10.1177/0961203308089444

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Citations (29)

Search for subsolar-mass black hole binaries in the second part of Advanced LIGO's and Advanced Virgo's third observing run

arXiv (Cornell University) (2022)

The LIGO Scientific Collaboration G. Losurdo the KAGRA Collaboration R. Abbott H. Abe

We describe a search for gravitational waves from compact binaries with at least one component with mass 0.2 $M_\odot$ -- $1.0 M_\odot$ and mass ratio $q \geq 0.1$ in Advanced LIGO and Advanced Virgo data collected between 1 November 2019, 15:00 UTC and 27 March 2020, 17:00 UTC. No signals were detected. The most significant candidate has a false alarm rate of 0.2 $\mathrm{yr}^{-1}$. We estimate the sensitivity of our search over the entirety of Advanced LIGO's and Advanced Virgo's third observing run, and present the most stringent limits to date on the merger rate of binary black holes with at least one subsolar-mass component. We use the upper limits to constrain two fiducial scenarios that could produce subsolar-mass black holes: primordial black holes (PBH) and a model of dissipative dark matter. The PBH model uses recent prescriptions for the merger rate of PBH binaries that include a rate suppression factor to effectively account for PBH early binary disruptions. If the PBHs are monochromatically distributed, we can exclude a dark matter fraction in PBHs $f_\mathrm{PBH} \gtrsim 0.6$ (at 90% confidence) in the probed subsolar-mass range. However, if we allow for broad PBH mass distributions we are unable to rule out $f_\mathrm{PBH} = 1$. For the dissipative model, where the dark matter has chemistry that allows a small fraction to cool and collapse into black holes, we find an upper bound $f_{\mathrm{DBH}} < 10^{-5}$ on the fraction of atomic dark matter collapsed into black holes.

Primordial black hole

Mass fraction

Mass ratio

10.1093/mnras/stad3120

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[Highly selective vagotomy for treatment of duodenal ulcer (author's transl)].

PubMed (1981)

Jiang Zm Yongliang Zhu H-Y. Huang Zeng Xj Chen Mz

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Influence of Postoperative Anemia on Functional Capacity Recovery in Patients Undergoing Coronary Artery Bypass Grafting

Journal of Vascular Medicine & Surgery (2016)

Hsu Cl Lai YL Wun TM Cheng Fh YS Lin

Background: Postoperative anemia (PPA) is a prevalent comorbidity after cardiac surgery. This study investigated the association between functional capacity (FC) recovery and postoperative anemia (PPA). Methods: We retrospectively analyzed 120 patients who received coronary artery bypass grafting. The lowest hemoglobin (Hb) data during hospitalization was collected. We divided the patients into two groups on the basis of hemoglobin (Hb) median value, defined as severe and mild anemia group. Peak oxygen consumption based on the results of a cardiopulmonary exercise test (CPXT) after discharge was used as the primary index of functional capacity (FC). For data analysis, T-test and Chi-Square test were used to compare group differences. Logistic regression was performed to compare the functional capacity recovery between groups. Linear regression was used to identify the effects of post-operation infusion in advanced. Statistical significance was a P value of 0.05. Results: Most of the patients were men (73.3%), and the mean age was 64.3 ± 10.5 years. All the patients had postoperative anemia (PPA) during hospitalization. The median value of lowest hemoglobin (Hb) during hospitalization was 8.8 g/dL. Patients in mild anemia group had 3.2-fold higher odds of functional capacity (FC) recovery to 3.5 METs when they discharge from hospital. Even post-operation infusion was given the severe anemia patients would still had worse functional capacity (FC) recovery. Conclusions: Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation Postoperative anemia (PPA) is a notable problem in CABG patients. Patients who have hemoglobin (Hb) levels <8.8 g/dL during hospitalization may not attain the expectedfunctional capacity (FC) after discharge.

10.4172/2329-6925.1000289

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