Lan Xu

Zhujiang Hospital

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Thor B. Nielsen

Uniformed Services University of the Health Sciences

Liying Sun

Chinese Academy of Medical Sciences & Peking Union Medical College

Pico Caroni

Friedrich Miescher Institute

Yong Gu

Binzhou University

Fleetwood A. Henry

Naval Medical Research Command

Dunja Frey

Friedrich Miescher Institute

Jungsil Ro

Dalhousie University

Rafal Ciosk

University of Oslo

Chuan‐Ming Hao

Huashan Hospital

Corinna Schneider

University Hospital Regensburg

Cooperative Institutions

Massachusetts General Hospital

John Wiley & Sons (United States)

Università Cattolica del Sacro Cuore

Virginia Commonwealth University

The University of Texas MD Anderson Cancer Center

Hudson Institute

University Health Network

Beth Israel Deaconess Medical Center

Mount Sinai Hospital

Faculty of Media

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Cellular Responses to Surgical Trauma, Hemorrhage, and Resuscitation with Diaspirin Cross-Linked Hemoglobin in Rats

Journal of Trauma and Acute Care Surgery (1997)

Lan Xu Liying Sun Florence M. Rollwagen Yingyue Li Nancy D. Pacheco

Background Resuscitation with acellular oxygen carrier solutions offers the potential advantage of improved oxygen delivery compared with crystalloid solutions, but the detailed consequences of improved resuscitation have not been fully evaluated. This study evaluated local and systemic cellular effects of trauma, hemorrhage, and resuscitation in a model of hemorrhage and surgical trauma. Methods Rats with a 10 cm full-thickness incisional wound and a 15 mL/kg hemorrhage were either not resuscitated or resuscitated with blood or diaspirin cross-linked hemoglobin (DCLHb). Cellular proliferative responses were evaluated at 1.5, 6, 24, and 48 hours after wounding by labeling in vivo with 5-bromo-2'-deoxyuridine. Plasma levels of interleukin-6, tumor necrosis factor-alpha, and interferon-gamma were measured by bioassay or enzyme-linked immunosorbent assay (ELISA). Bacterial translocation was measured by culturing liver homogenates. Results Trauma inhibited keratinocyte and hepatocyte proliferation at 1.5 and 6 hours, and stimulated subsequent proliferation of keratinocytes and liver nonparenchymal cells. DCLHb stimulated wound keratinocyte proliferation, attenuated the inhibition of hepatocyte proliferation, eliminated bacterial translocation to the liver, protected the intestine from ischemic damage, and induced a rapid increase of interleukin-6 during the early phase of injury. Conclusions Surgical trauma alone, or in combination with hemorrhage, modulated cell proliferation both in the wound and in the remote organs of intestine and liver. DCLHb enhanced wound healing and cell proliferation as well as, or better than, freshly drawn blood, which may be beneficial for trauma care.

10.1097/00005373-199701000-00007

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Citations (32)

Molecular characterization of the mouse ribosomal protein S24 multigene family: a uniquely expressed intron-containing gene with cell-specific expression of three alternatively spliced mRNAs

Nucleic Acids Research (1994)

Lan Xu Gongping He Audrey Li Jungsil Ro

Journal Article Molecular characterization of the mouse ribosomal protein S24 multigene family: a uniquely expressed intron-containing gene with cell-specific expression of three alternatively spliced mRNAs Get access Lan Xu, Lan Xu Department of Biochemistry, Faculty of MedicineDalhousie University, Halifax, Nova Scotia B3H 4H7, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Gong-Ping He, Gong-Ping He Department of Biochemistry, Faculty of MedicineDalhousie University, Halifax, Nova Scotia B3H 4H7, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Audrey Li, Audrey Li Department of Biochemistry, Faculty of MedicineDalhousie University, Halifax, Nova Scotia B3H 4H7, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Hyo-Sung Ro Hyo-Sung Ro Department of Biochemistry, Faculty of MedicineDalhousie University, Halifax, Nova Scotia B3H 4H7, Canada *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 22, Issue 4, 25 February 1994, Pages 646–655, https://doi.org/10.1093/nar/22.4.646 Published: 25 February 1994 Article history Received: 02 September 1993 Accepted: 01 January 1994 Published: 25 February 1994

Nova scotia

10.1093/nar/22.4.646

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Citations (31)

Issue Information

Cancer Cytopathology (2016)

Jane Gibson Celeste N. Powers Martha B. Pitman Tommaso Bizzarro Maurizio Martini

The past 20 years in cytopathology have demonstrated tremendous growth and evolution in the cytopathology of organs located below the diaphragm, with much of the progress centering on the pancreas.Cancer Cytopathology has been at the forefront of reporting advances in the field of pancreaticobiliary cytology, especially for the diagnosis of pancreatic cysts.

10.1002/cncy.21678

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Selective vulnerability and pruning of phasic motoneuron axons in motoneuron disease alleviated by CNTF

Nature Neuroscience (2006)

San Pun Alexandre Ferrão Santos Smita Saxena Lan Xu Pico Caroni

Ciliary neurotrophic factor

10.1038/nn1653

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Citations (612)

Nucleotide sequences of a cDNA clone encoding mouse ribosomal protein S24

Nucleic Acids Research (1991)

Jungsil Ro Lan Xu

Journal Article Nucleotide sequences of a cDNA clone encoding mouse ribosomal protein S24 Get access Hyo-Sung Ro, Hyo-Sung Ro Department of Biochemistry, Faculty of Medicine, Dalhousie UniversityHalifax, Nova Scotia, B3H 4H7, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Lan Xu Lan Xu Department of Biochemistry, Faculty of Medicine, Dalhousie UniversityHalifax, Nova Scotia, B3H 4H7, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 19, Issue 23, 11 December 1991, Page 6647, https://doi.org/10.1093/nar/19.23.6647 Published: 11 December 1991 Article history Received: 18 September 1991 Published: 11 December 1991

Nova scotia

Ribosomal protein

clone (Java method)

10.1093/nar/19.23.6647

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Citations (6)

Expression of nestin in the podocytes of normal and diseased human kidneys

AJP Regulatory Integrative and Comparative Physiology (2007)

Wei Su Jing Chen Haichun Yang You Li Lan Xu

The complex cyto-architecture of the podocyte is critical for glomerular permselectivity. The present study characterizes the expression of nestin, an intermediate filament protein, in human kidneys. In normal kidneys, nestin was detected at the periphery of glomerular capillary loops. Colabeling showed nestin was expressed in WT1-positive cells. Within the podocyte, nestin immunoreactivity was present in the cell body and primary process. This was supported by immunoelectron microscopy. Nestin also colocalized with vimentin in the periphery of capillary loops but not in the mesangium. Nestin was not detected in other structures of the adult human kidney. To determine the potential role of nestin in proteinuria, nestin was examined in kidney biopsies from patients with or without proteinuria. These patients were diagnosed with IgA nephropathy with mild mesangial expansion but without proteinuria, IgA nephropathy with proteinuria, membranous nephropathy (MN), and focal segmental glomerular sclerosis (FSGS). The distribution of nestin in these biopsies was similar to that in the normal kidney. Semiquantitative analysis of immunostaining showed that glomerular nestin expression in IgA nephropathy without proteinuria was not different from normal kidney; however, nestin expression in kidneys of patients with IgA nephropathy and proteinuria, or MN and FSGS with proteinuria was significantly reduced compared with normal kidney (P < 0.01). Reduced nestin mRNA expression in the patients with IgA nephropathy with proteinuria and FSGN was also observed by quantitative real-time PCR. These studies suggest that nestin may play an important role in maintaining normal podocyte function in the human kidney.

Nestin

Membranous Nephropathy

Mesangium

Glomerulus

Glomerular mesangium

10.1152/ajpregu.00319.2006

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Citations (38)

Transfection with aFGF cDNA Improves Wound Healing

Journal of Investigative Dermatology (1997)

Liying Sun Lan Xu Henry S. Chang Fleetwood A. Henry Robert M. Miller

10.1111/1523-1747.ep12286471

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Citations (101)

Early and Selective Loss of Neuromuscular Synapse Subtypes with Low Sprouting Competence in Motoneuron Diseases

Journal of Neuroscience (2000)

Dunja Frey Corinna Schneider Lan Xu Jacques Borg Will Spooren

The addition or loss of synapses in response to changes in activity, disease, or aging is a major aspect of nervous system plasticity in the adult. The mechanisms that affect the turnover and maintenance of synapses in the adult are poorly understood and are difficult to investigate in the brain. Here, we exploited a unique anatomical arrangement in the neuromuscular system to determine whether subtypes of synapses can differ in anatomical plasticity and vulnerability. In three genetic mouse models of motoneuron disease of diverse origin and severity, we observed a gradual and selective loss of synaptic connections that begun long before the onset of clinical deficits and correlated with the timing of disease progression. A subgroup of fast-type (fast-fatiguable) neuromuscular synapses was highly vulnerable and was lost very early on. In contrast, slow-type synapses resisted up to the terminal phase of the disease. Muscle-specific differences were also evident. Similar selective losses were detected in aged mice. These selective vulnerability properties of synapses coincided with hitherto unrecognized major differences in stimulus-induced anatomical plasticity that could also be revealed in healthy mice. Using paralysis and/or growth-associated protein 43 overexpression to induce synaptic sprouting, we found that slow-type, disease-resistant synapses were particularly plastic. In contrast, fast-type synapses with the highest vulnerability failed to exhibit any stimulus-induced change. The results reveal pronounced subtype specificity in the anatomical plasticity and susceptibility to loss of neuromuscular synapses and suggest that degenerative motoneuron diseases involve a common early pathway of selective and progressive synaptic weakening also associated with aging.

Stimulus (psychology)

10.1523/jneurosci.20-07-02534.2000

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Citations (651)

LIN41 Post-transcriptionally Silences mRNAs by Two Distinct and Position-Dependent Mechanisms

Molecular Cell (2017)

Florian Aeschimann Pooja Kumari Hrishikesh Bartake Dimos Gaidatzis Lan Xu

RNA Silencing

10.1016/j.molcel.2016.12.010

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Citations (80)

Shared and Unique Roles of Cap23 and Gap43 in Actin Regulation, Neurite Outgrowth, and Anatomical Plasticity

The Journal of Cell Biology (2000)

Dunja Frey Thorsten Laux Lan Xu Corinna Schneider Pico Caroni

CAP23 is a major cortical cytoskeleton-associated and calmodulin binding protein that is widely and abundantly expressed during development, maintained in selected brain structures in the adult, and reinduced during nerve regeneration. Overexpression of CAP23 in adult neurons of transgenic mice promotes nerve sprouting, but the role of this protein in process outgrowth was not clear. Here, we show that CAP23 is functionally related to GAP43, and plays a critical role to regulate nerve sprouting and the actin cytoskeleton. Knockout mice lacking CAP23 exhibited a pronounced and complex phenotype, including a defect to produce stimulus-induced nerve sprouting at the adult neuromuscular junction. This sprouting deficit was rescued by transgenic overexpression of either CAP23 or GAP43 in adult motoneurons. Knockin mice expressing GAP43 instead of CAP23 were essentially normal, indicating that, although these proteins do not share homologous sequences, GAP43 can functionally substitute for CAP23 in vivo. Cultured sensory neurons lacking CAP23 exhibited striking alterations in neurite outgrowth that were phenocopied by low doses of cytochalasin D. A detailed analysis of such cultures revealed common and unique functions of CAP23 and GAP43 on the actin cytoskeleton and neurite outgrowth. The results provide compelling experimental evidence for the notion that CAP23 and GAP43 are functionally related intrinsic determinants of anatomical plasticity, and suggest that these proteins function by locally promoting subplasmalemmal actin cytoskeleton accumulation.

Neurite

Gap-43 protein

Sprouting

10.1083/jcb.149.7.1443

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Citations (272)