F. Escaig

École Nationale Vétérinaire d'Alfort

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Peter R. Galle

University Medical Center of the Johannes Gutenberg University Mainz

J. P. Berry

Inserm

Pedro Antônio Oliveira Mangabeira

Universidade Estadual de Santa Cruz

L. Labéjof

Universidade Estadual de Santa Cruz

C. Chassard-Bouchaud

Sorbonne Université

Marcelo Schramm Mielke

Universidade Estadual de Santa Cruz

M. I. Severo

Universidade Estadual de Santa Cruz

P. Boumati

Centre National de la Recherche Scientifique

Arno Heeren de Oliveira

Universidade Federal de Minas Gerais

Kaline Benevides Santana

Universidade Estadual de Santa Cruz

Cooperative Institutions

Centre National de la Recherche Scientifique

Inserm

Université Paris Cité

Sorbonne Université

Universidade Estadual de Santa Cruz

Université Paris-Est Créteil

Université Paris-Sud

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Laboratoire d'études sur les monothéismes

D.I. Ivanovsky Institute of Virology Russian Academy of Medical Sciences

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Standards study for quantitative analysis of trace elements in biological tissue using secondary ion mass spectrometry

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society (1992)

Pierre Boumati F. Escaig F. Sommer R.L. Inglebert Simon A. Mountfort

Trials have been carried out to establish appropriate standards for quantitative biological microanalysis using SIMS. This feasibility study has demonstrated that gelatin and ion exchange resin microbeads embedded in epoxy resin could be homogenously loaded with Al in a large range of concentrations (up to 2000 ppm). We have obtained good linear correlations between trace element / internal reference element ratio and trace element concentrations verified by chemical analysis techniques. Furthermore, it has been possible to determine unknown concentrations with precision within one standard deviation of the chemically determined concentrations.

Microanalysis

Trace element

Quantitative Analysis

TRACE (psycholinguistics)

Ion-exchange resin

Relative standard deviation

10.1109/iembs.1992.5760870

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Analytical subcellular distribution of cAMP‐dependent protein kinase activity in bull sperm

Gamete Research (1984)

Claude Pariset Jacqueline S. Weinman F. Escaig Michèle Guyot Francine Iftode

Abstract The distribution of the cAMP‐dependent protein kinase activity in bull ejaculated sperm has been investigated. This activity proved to be mainly present in a soluble form inside the cell. Sperm fractionation into heads and flagellar fragments, using differential centrifugation or centrifugal elutriation, has shown that the particulate cAMP‐dependent protein kinase activity was mainly associated with the flagellar structures. A much activity was shown to be associated with the head fraction. Some activity could also be detected in the purified plasma membrane fraction.

Elutriation

Cell fractionation

Differential centrifugation

Capacitation

10.1002/mrd.1120100409

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Citations (3)

Rapid localization of carbon 14-labeled molecules in biological samples by ion mass microscopy.

Journal of Histochemistry & Cytochemistry (1989)

Elif Hindié F. Escaig Bernard Coulomb Corinne Lebreton Peter R. Galle

We report here on the ability of secondary ion mass spectrometry (SIMS) to provide rapid imaging of the intracellular distribution of 14C-labeled molecules. The validity of this method, using mass discrimination of carbon 14 atoms, was assessed by imaging the distribution of two molecules of well-known metabolism, [14C]-thymidine and [14C]-uridine, incorporated by human fibroblasts in culture. As expected, 14C ion images showed the presence of [14C]-thymidine in the nucleus of dividing cells, whereas [14C]-uridine was present in the cytoplasm as well as the nucleus of all cells, with a large concentration in the nucleoli. The time required to obtain the distribution images with the SMI 300 microscope was less than 6 min, whereas microautoradiography, the classical method for mapping the tissue distribution of 14C-labeled molecules, usually requires exposure times of several months. Secondary ion mass spectrometry using in situ mass discrimination is proposed here as a very sensitive method which permits rapid imaging of the subcellular distribution of molecules labeled with carbon 14.

Thymidine

Mass spectrometry imaging

10.1177/37.2.2911003

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Citations (9)

Subcellular localization of aluminum and indium in the rat kidney

Applied Surface Science (2004)

Peter R. Galle R. Levi‐Setti Alessio Lamperti Khadidja Bourahla F. Escaig

10.1016/j.apsusc.2004.03.183

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Citations (25)

DISTRIBUTION DU GALLIUM DANS DIFFÉRENTS TISSUS DE MAMMIFÈRES - ÉTUDE EN MICROANALYSE PAR ÉMISSION IONIQUE SECONDAIRE ET PAR SPECTROMÉTRIE DE RAYONS X

Le Journal de Physique Colloques (1984)

John P. Berry S. Galle F. Escaig

10.1051/jphyscol:19842122

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Citations (4)

Localisation intracellulaire du beryllium dans les cellules de rein et du poumon

Biology of the Cell (1988)

John P. Berry F. Escaig Peter R. Galle

10.1016/0248-4900(88)90219-5

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[Bioaccumulation of lithium by marine organisms in European, American, and Asian coastal zones: microanalytic study using secondary ion emission].

PubMed (1984)

C Chassard-Bouchaud Peter R. Galle F. Escaig Mitsuharu Miyawaki

37 species of aquatic mammals, fish, crustacea, annelids, molluscs belonging to cephalopods, gasteropods and lamellibranchs were collected from coastal waters of France: North Sea, English Channel, Atlantic Ocean, Mediterranean Sea, from Greece: Aegian Sea, from North America: Atlantic Ocean and from Japan: Pacific Ocean, Sea of Japan and East China Sea. Microanalyses which were performed on organs and tissues, using secondary ion mass spectrometry, revealed high concentrations of lithium, which is commonly used in human therapy, but is also toxic in low amounts. The retention of this metal by the marine organisms appears as a general phenomenon independent of their biotope and geographical origin; the highest lithium levels were detected in the fish muscles (= edible part).

Biotope

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The use of SIMS for uranium localization in biological research

Journal of Alloys and Compounds (1998)

Ademir Amaral Peter R. Galle F. Escaig C. Cossonnet M.H. Hengé-Napoli

10.1016/s0925-8388(98)00016-4

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Citations (5)

[The detection of viral antigens and ribosomal RNA in cells by using low-temperature media].

PubMed (1990)

Grigor'ev Vb Kadoshnikov IuP F. Escaig Fournier Jg Rudneva Ia

A post-embedding technique for immunocytochemical analysis at the ultrastructural level was used to detect and localize HIV antigens on ultrathin sections of Lowicryl-embedded HIV-infected cells. A genomic probe containing ribosomal sequences and labeled with biotin was used to hybridize rRNA molecules in sections of animal cells embedded in Lowicryl. The method presently described offers the possibility to detect rapidly and precisely ribosomal gene expression and viral proteins at the ultrastructural level.

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Données nouvelles sur la structure et la minéralisation des écailles d'Anguilla anguilla (Osteichthyes, Anguillidae)

Canadian Journal of Zoology (1984)

Louise Zylberberg François Meunier F. Escaig Sylvain Halpern

The structure of the scales in Anguilla anguilla was studied by light and electron microscopy and the mineral phase was analyzed by histophysical techniques. Although the scales of the eel are small in size and have a peculiar superficial ornamentation, which consists of a web of mineralized plates, they are composed of the three characteristic layers of the typical elasmoid scale. The discontinuous, superficial outer limiting layer is highly mineralized; in this layer, acid mucosubstances are abundant but collagen fibres are absent. Mineralization in the external layer is not oriented by the network of the thin collagen fibrils. In the basal plate, mineralization is oriented by the thick collagen fibres packed in bundles. The bundles are oriented from the basal part to the surface of the scale but they are not organized in the laminated structure characteristic of most other teleost scales. The mineral phase of the eel is typical of a teleost fish. The peculiar features of the scale in the eel are interpreted here as a discrete example of the phyletical reduction of the dermal skeleton among Actinopterygii.

Actinopterygii

Anguillidae

Limiting

10.1139/z84-365

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Citations (11)