In order to investigate the influence of X-irradiation upon the urease, the authors performed several experiments and obtained the following conclusion:1) The urease action are influenced by X-irradiation.2) The activity of urease is strengthened in the case of irradiation with from 40r to 120r, and inhibited in the case of irradiation ranging from 140r to 200r or 400r.3) The effect of X-irradiation upon urease action is caused by the irradiation of urease itself, and not of the substrate (urea).4) When KCN is added to the urease urea mixture, the urease activity is activated in proportion to the concentration of KCN. In the case of an addition of KCN to the urease urea mixture, the effect of X-irradiation upon the urease activity is slight in case of strong concentration of KCN, but appears distinctly in case of weak concentration of KCN.5) When CuSO4 is added to the urease urea mixture, the urease activity is inhibited in proportion to the concentration of CuSO4. In the case of addition of CuSO4 to the urease urea mixture, the effect of X-irradiation upon the urease activity is seen slightly in case of strong concentration of CuSO4, but distinctly in case of weak concentration of CuSO4.6) In the case of solar radiation, the urease activity is inhibited in proportion to the time of solar radiation, and the urease activity which is inhibited by solar radiation for 30 minutes, is reactivated by X-irradiation with 80r.
We examined the incidence of non-A, non-B hepatitis (NANB) in post-transfusion hepatitis in uterine cancer patients treated by surgery at our hospital. The diagnosis of NANB was based on the diagnostic standards for post-transfusion hepatitis of the Japanese Society of Gastroenterology 1985. In 587 patients with blood transfusions from 1978 to 1988, 80 patients (13.6%) were diagnosed as NANB. The proportions of blood components were, preserved whole blood 44.1%, concentrated red blood cells (CRBC)+ fresh frozen plasma 31.2%, and CRBC 12.8%. The proportions of blood components changed over the years, but since 1987 those of blood components in blood transfusion have been greatly decreased. No difference in the incidence of NANB was seen in patients with up to 10 units of blood transfused, but a high incidence was observed to patients who had received over 10 units. The number of patients treated by radical hysterectomy was five hundred and sixty-two (95.7%) out of a total of five hundred and eighty seven. The average blood loss in the operation was 1,392 ml.
Tumor angiogenesis is essential for solid tumor growth. The object of this study was to investigate the presence of newly identified angiogenic factor, placenta growth factor (P1GF) in human cervical cancers. The expression of P1GF mRNA was assessed by RT-PCR in 29 patients with cervical cancer. Fifteen out of 29 cervical cancers expressed a certain level of P1GF mRNA. In addition, the expression levels of P1GF mRNA in squamous cell carcinomas were significantly higher than those in adenocarcinomas. There was no correlation between the expression of P1GF mRNA and FIGO stage. These results indicate that the expression of P1GF may be implicated in the promotion of angiogenesis in human cervical squamous cell carcinomas, but not in human cervical adenocarcinomas.
