Xiangming Gao

The Fourth People's Hospital of Ningxia Hui Autonomous Region

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Co-Authors

Samer Al‐Murrani

Hill Top Research (United States)

Ryan M. Yamka

Luna Innovations (United States)

K G Friesen

Nutrasource

N Z Frantz

The Mountain Institute

Steve C Zicker

Hill's Pet Nutrition (United States)

Olivier Destrée

Hubrecht Institute for Developmental Biology and Stem Cell Research

S. B. Malladi

University of Kansas

Joselin Peterson-Maduro

Hubrecht Institute for Developmental Biology and Stem Cell Research

Mary Locniskar

Hill Top Research (United States)

Eric Kalkhoven

University Medical Center Utrecht

Cooperative Institutions

Hill Top Research (United States)

Hill's Pet Nutrition (United States)

Yinchuan First People's Hospital

McGill University

Chinese Academy of Sciences

Kansas State University

Shriners Hospitals for Children - Canada

Peking University

Hubrecht Institute for Developmental Biology and Stem Cell Research

Montreal Children's Hospital

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Identification of genes altered with maternal nutrition in growing puppies

The FASEB Journal (2007)

N Z Frantz Steve C Zicker K G Friesen Ryan M. Yamka Xiangming Gao

Maternal nutrition may influence proper growth and development as well as the occurrence of disease. Providing specific nutrients early in life may improve quality of life in dogs. Sixteen beagle puppies were utilized in this study. Dams and puppies were fed either a commercially available food or Hill's Science Diet® from conception to one year of age. RNA was extracted from blood and hybridized to an Affymetrix GeneChip Canine-2 Genome Arrays and normalized using Robust Multi-Array Average. Transcripts having a P < 0.05 (following a false discovery rate adjustment value of 0.1) and a fold change range of at least 1.5 were considered different among the two groups. Analysis of the RNA found differences in 143 genes between puppies fed Hill's Science Diet® compared to the commercial food. Of the genes identified, 90 were down-regulated and 53 up-regulated in Hill's Science Diet® fed puppies versus puppies fed the commercial food. Genes associated with, DNA replication and repair, cell compromise, arthritis, and cancer were decreased, while cellular assembly and cell cycle regulation were increased in puppies fed Hill's Science Diet® compared to puppies fed the commercial food.

Beagle

10.1096/fasebj.21.5.a4-a

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HES6 acts as a transcriptional repressor in myoblasts and can induce the myogenic differentiation program

The Journal of Cell Biology (2001)

Xiangming Gao Tanya Chandra Michel‐Olivier Gratton Isabelle Quélo Josée Prud’homme

HES6 is a novel member of the family of basic helix–loop–helix mammalian homologues of Drosophila Hairy and Enhancer of split. We have analyzed the biochemical and functional roles of HES6 in myoblasts. HES6 interacted with the corepressor transducin-like Enhancer of split 1 in yeast and mammalian cells through its WRPW COOH-terminal motif. HES6 repressed transcription from an N box–containing template and also when tethered to DNA through the GAL4 DNA binding domain. On N box–containing promoters, HES6 cooperated with HES1 to achieve maximal repression. An HES6–VP16 activation domain fusion protein activated the N box–containing reporter, confirming that HES6 bound the N box in muscle cells. The expression of HES6 was induced when myoblasts fused to become differentiated myotubes. Constitutive expression of HES6 in myoblasts inhibited expression of MyoR, a repressor of myogenesis, and induced differentiation, as evidenced by fusion into myotubes and expression of the muscle marker myosin heavy chain. Reciprocally, blocking endogenous HES6 function by using a WRPW-deleted dominant negative HES6 mutant led to increased expression of MyoR and completely blocked the muscle development program. Our results show that HES6 is an important regulator of myogenesis and suggest that MyoR is a target for HES6-dependent transcriptional repression.

Corepressor

LIM domain

10.1083/jcb.200104058

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Identification of age‐related changes in gene expression in adult versus geriatric dogs

The FASEB Journal (2008)

N Z Frantz Ryan M. Yamka K G Friesen Xiangming Gao Samer Al‐Murrani

Thirty adult (<10 years of age) and thirty geriatric (>10 years of age) neutered/spayed beagles were identified for this study. Animals were fed a control food for 30 days and then a blood sample was drawn into a PAXgene Blood RNA tube. RNA was extracted according to the procedures provided in the PAXgene Blood RNA Kit Handbook (Qiagen, Valencia, CA). RNA was hybridized to an Affymetrix GeneChip Canine‐2 Genome Arrays and normalized using Robust Multi‐Array Average. Transcripts having a P < 0.01 (following a false discovery rate adjustment value of 0.1) and a fold change range of at least 1.5 were considered different among the two groups. Analysis of the RNA found differences in 580 genes between the geriatric and adult groups. Of the genes identified, 310 were up‐regulated and 270 down‐regulated in geriatric dogs when compared to adult dogs. Genes associated with immune system activation, cancer formation, B and T‐cell receptor signaling, protein ubiquitination, and branched chain amino acid degradation were increased, while cell‐cell signaling, cell cycle regulation, and blood coagulation were decreased in geriatric compared to adult dogs.

Fold change

10.1096/fasebj.22.1_supplement.444.7

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Identification of genes altered with growth phase nutrition in growing puppies

The FASEB Journal (2007)

N Z Frantz Steve C Zicker K G Friesen Ryan M. Yamka Xiangming Gao

Providing proper nutrition during early growth may influence the propensity to develop disease later in life and is critical for proper development of puppies. Sixteen beagle puppies were allotted to a commercially available food or Hill's Science Diet® from weaning to one year of age. RNA was extracted from blood and hybridized to an Affymetrix GeneChip Canine-2 Genome Arrays and normalized using Robust Multi-Array Average. Transcripts having a P < 0.05 (following a false discovery rate adjustment value of 0.1) and a fold change range of at least 1.5 were considered different among the two groups. Analysis of the RNA found differences in 99 genes between the Hill's Science Diet® fed puppies and puppies fed the commercial food. Of the genes identified, 32 were up-regulated and 67 down-regulated in Hill's Science Diet® fed puppies when compared to puppies fed the commercial food. Genes associated with, immune system activation, cardiovascular development, lipid metabolism, skeletal and muscular disorders, cellular compromise, and cancer were decreased, while muscle contraction increased in puppies fed Hill's Science Diet® compared to the commercial food.

Beagle

10.1096/fasebj.21.5.a4-b

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Bioavailability of tea catechins and their effect on gene expression in dogs

The FASEB Journal (2007)

Inke Paetau‐Robinson Mary Locniskar Xiangming Gao Samer Al‐Murrani

Green tea is believed to positively affect weight management, risk of cardiovascular disease, and risk of cancer. In this study we determined the absorption of catechins from green tea and their effect on gene expression in white blood cells in dogs. Eighteen Beagles (2 – 9 yrs), randomized to two treatment groups, participated in the 1-day study. The dogs either received the control diet or the control diet plus green tea extract. The green tea was added to the meal prior to feeding and provided 76 mg epicatechins/kg body weight. Blood was collected prior to feeding, and at hour 1.5, 2.5, 6, and 24 post-feeding. Plasma catechins were analyzed at all time points. White blood cells were isolated from the blood samples collected prior to feeding, and at 6 and 24 hours post-feeding for gene expression analysis. The peak plasma catechin concentrations achieved by feeding green tea occurred between 1.5 hr and 2.5 hr post-feeding and were 31 μg/L, 23 μg/L, 98 μg/L, and 59 μg/L for epicatechin, epicatechin gallate, epigallocatechin, and epigallocatechin gallate, respectively. Following ingestion of green tea, 560 genes were differentially expressed including genes that are associated with lipid metabolism, cell cycle regulation, and cancer. We can conclude that tea catechins are absorbed from green tea extract in dogs. Based on gene expression data green tea has an effect on lipid metabolism and cell cycle regulation.

Green tea extract

White blood cell

Epicatechin gallate

10.1096/fasebj.21.6.a744

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Differential gene expression of naturally occurring malignant and benign canine mammary tumors

The FASEB Journal (2007)

Inke Paetau‐Robinson Mary Locniskar Xiangming Gao Samer Al‐Murrani

Cancer in companion animals is one of the leading causes of non-accidental death. Understanding the gene expression profile in tumors can help to develop effective treatments and interventions to prolong and improve the life of companion animals with cancer. In this study gene expression of naturally occurring mammary tumors, 46 complex adenomas (benign) and 19 adenocarcinomas (malignant), collected from female dogs were compared. The mammary tumors were removed as part of normal health care by standard surgical procedures from female beagles aged between 5 and 15 years. The center part of the tumor was snap frozen in liquid nitrogen. RNA was isolated from the tumor tissues and applied to Affymetrix GeneChip® Canine Genome 2.0 Array. We found that 302 genes were differentially expressed. In the malignant mammary tumors, 149 genes were up-regulated and 153 genes were down-regulated compared to the benign tumors. Genes associated with cell cycle regulation, cell division, and cell remodeling were affected. Based on these findings it can be concluded that malignant tumors have a more active cell division machinery compared to benign tumors.

Mammary tumor

10.1096/fasebj.21.6.a743-b

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Identification of gene changes in geriatric dogs fed a test or control food

The FASEB Journal (2007)

N Z Frantz Ryan M. Yamka K G Friesen Xiangming Gao Samer Al‐Murrani

Many common diseases are associated with the aging process. Using genomics to determine the influence of nutrition on these processes may potentially lead to nutritional enhancement of healthy aging for dogs. Twenty geriatric (>10 years of age) beagles were fed a control food for 28 days and then allotted to either a control geriatric food or a test food (enhanced amino acid balance, essential fatty acids) for 14 days. RNA was extracted from blood and hybridized to an Affymetrix GeneChip Canine-1 Genome Arrays and normalized using Robust Multi-Array Average. Transcripts having a P < 0.01 (false discovery rate adjustment value of 0.1) and a fold change range of at least 1.5 were considered different among the two groups. Analysis of the RNA found differences in 1088 genes between dogs fed the experimental and basal foods. Of the genes identified, 10 were up-regulated and 1078 down-regulated in geriatric dogs fed the test food compared to the control food. Genes associated with arthritis/inflammation, pain, blood coagulation, glucose metabolism, and DNA damage were down-regulated in dogs fed a test geriatric food compared to a control geriatric food which suggests improved quality of life.

10.1096/fasebj.21.6.a744-c

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Feline degenerative joint disease: a genomic and proteomic approach

Journal of Feline Medicine and Surgery (2013)

Xiangming Gao Junyu Lee S. B. Malladi Lynda Melendez B. Duncan X. Lascelles

The underlying disease mechanisms for feline degenerative joint disease (DJD) are mostly unidentified. Today, most of what is published on mammalian arthritis is based on human clinical findings or on mammalian models of human arthritis. However, DJD is a common occurrence in the millions of domestic felines worldwide. To get a better understanding of the changes in biological pathways that are associated with feline DJD, this study employed a custom-designed feline GeneChip, and the institution's unique access to large sample populations to investigate genes and proteins from whole blood and serum that may be up- or down-regulated in DJD cats. The GeneChip results centered around three main pathways that were affected in DJD cats: immune function, apoptosis and oxidative phosphorylation. By identifying these key disease-associated pathways it will then be possible to better understand disease pathogenesis and diagnose it more easily, and to better target it with pharmaceutical and nutritional intervention.

Joint disease

10.1177/1098612x12470652

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Identification of genes related to obesity in dogs

The FASEB Journal (2007)

Ryan M. Yamka K G Friesen Xiangming Gao Samer Al‐Murrani

Forty-two neutered/spayed beagles (average age = 7.5 ± 2.8 years) were identified for this study. Dogs were weighed, given a body condition score (BCS; 1=lean, 3=ideal and 5=obese) and a blood sample was drawn into a PAXgene blood RNA tube. Of the forty-two beagles identified, 12 were classified as lean (BCS = 2.2 ± 0.3; BW = 10.5 ± 1.9 kg) and 30 as overweight (BCS = 4.3 ± 0.4; BW = 15.2 ± 3.3 kg). RNA was extracted according to the procedures provided in the PAXgene Blood RNA Kit Handbook (Qiagen, Valencia, CA). RNA was hybridized to an Affymetrix Gene Chip Canine-2 Genome Array and normalized using Robust Multi-Array Average. Transcripts having a P < 0.01 (following a false discovery rate adjustment value of 0.1) and > 1.3-fold change were considered different among the two groups. Analysis of the RNA found differences in 815 genes between the two groups. Of the genes identified, 59 were expressed higher and 756 were lower in the overweight group when compared to the lean group. The genes with higher expression in overweight dogs were associated with fatty acid metabolism, purine metabolism and platelet derived growth factor signaling. The genes with lower expression in overweight dogs were associated with nucleotide metabolism, carbohydrate metabolism, interleukin signaling, PPAR signaling, IGF-1 signaling, insulin receptor signaling, amino acid metabolism, branch chain amino acid degradation and lipid metabolism.

Fold change

Fatty Acid Metabolism

10.1096/fasebj.21.5.a4-d

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Laboratory indices in patients with osteonecrosis of the femoral head: a retrospective comparative study

Journal of Orthopaedic Surgery and Research (2023)

Haotian Zheng Binglin Ye K. L. Huang Xiangming Gao Wei Chen

Osteonecrosis of the femoral head is a degenerative condition linked to corticosteroids, alcoholism, or trauma. With its rising prevalence due to increased hormone drug use and its debilitating effects on young to middle-aged individuals, understanding its association with specific laboratory indicators can aid early diagnosis and prevention.Upon retrospective analysis of the clinical data pertaining to individuals diagnosed with femoral head necrosis, spanning from January 2016 to January 2022, a comprehensive evaluation was conducted within the same time frame. The study aimed to ascertain the presence of femoral head necrosis in a total of 1176 individuals. A total of 1036 healthy patients were recruited randomly, ensuring that their ages matched. The risk variables associated with the utilization of logistic regression analysis and analysis techniques are employed. The patient examines the age distribution within a specific age group.The levels of high-density lipoprotein, low-density lipoprotein A1, lipoprotein B1, total protein, albumin, globulin, and other lipophilic metabolism and coagulation markers exhibited a statistically significant increase compared to the control group. A multifactor logistic regression analysis was conducted to identify potential risk factors associated with femoral head necrosis in patients.Femoral head necrosis is associated with a range of variables including coagulation malfunction, lipid metabolic abnormalities, and inflammation.

Avascular Necrosis

10.1186/s13018-023-04235-0

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Citations (2)