S. Uppal

Chaudhary Charan Singh Haryana Agricultural University

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Anita R. Sehrawat

Maharshi Dayanand University

Pushpa Kharb

Chaudhary Charan Singh Haryana Agricultural University

Charu Mitra

University of Engineering & Management

Sunita Jain

Public Library of Science

Krishan D. Sehrawat

Chaudhary Charan Singh Haryana Agricultural University

Parveen Batra

V. K. Chowdhury

Chaudhary Charan Singh Haryana Agricultural University

Santosh Dhillon

Chaudhary Charan Singh Haryana Agricultural University

Kuldeep Singh

Integral University

Sashi Kant

University of Colorado Denver

Cooperative Institutions

Chaudhary Charan Singh Haryana Agricultural University

Maharshi Dayanand University

Kurukshetra University

Academy of Scientific and Innovative Research

Panjab University

Punjab Agricultural University

Oil and Natural Gas Corporation (India)

National Dairy Research Institute

Institute of Engineering

Dr. Yashwant Singh Parmar University of Horticulture and Forestry

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Evaluation of callus cultures of Glycyrrhiza glabra L. var. HM 1 for glycyrrhizin.

National Journal of Plant Improvement (2005)

Pinakee D. Sharma S. Uppal Anita R. Sehrawat

Glycyrrhizin

Glycyrrhiza

Callus

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Citations (1)

Response of wheat crosses involving salt tolerant genotypes to anther culture

Indian Journal of Genetics and Plant Breeding (The) (2002)

Anita R. Sehrawat S. Uppal Kuldeep Singh

Anthers of seven wheat (Triticum aestivum L) genotypes and their eight F1 hybrids involving Kharchia375 and Kharchia65, salt tolerant cultivars, as female parents were cultured on four modified media, both solid and liquid. The per cent anthers forming calli varied between 0 and 9.69 depending upon the genotype, cross and culture medium. Calli yielded both green (upto 5.6%) and albino plantlets (8.5 to 48.9%) on transfer to solidified agar 190-2 regeneration medium. Crosses differed significantly and the best cross with respect to callusing and green plantlet regeneration was Kharchia375 x HD2009. In general responses of different crosses were significantly better in liquid media and the most effective medium overall was P2 followed by P4, BAC1 and Heh. Higher green plantlet regeneration abilities from anthers of F1 hybrids as compared to the parents suggest their use to increase green plant yield. Plantlets thus produced from crosses after diploidization were transferred to pots. Ninety-seven fertile spikes obtained from crosses showed a wide variation for spike length, spikelet number per spike and grain number per spike. The study may be helpful for the development of salt tolerant doubled haploid lines of wheat.

Plantlet

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Early Diagnosis of Sex in Jojoba, Simmondsia chinensis (Link) Schneider by Sequence Characterized Amplified Region Marker

Proceedings of the National Academy of Sciences India Section B Biological Sciences (2013)

Surender Jangra Pushpa Kharb Charu Mitra S. Uppal

Link (geometry)

Sequence (biology)

10.1007/s40011-013-0226-2

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Citations (7)

Efficient callus induction, plant regeneration and their selection at different NaCl levels in wheat crosses

Crop Research (2012)

Anita R. Sehrawat S. Uppal Krishan D. Sehrawat Subhash Kajla

The experiment encompasses the in vitro culture of immature embryo of nine F1 hybrids including two salt tolerant cultivars Kharchia 375 and Kharchia 65, which were used as female parents. Initially all embryos were cultured on MS medium supplemented with 2, 4-D (5.0 mg/l) for callus formation. Proliferation of the callus on MS medium was fortified with 2, 4-D (3.0 mg/l). The callusing percentage among different crosses varied from 86.85-97.20%. These calli were placed on callus selection medium with graded concentrations of NaCl (4, 12, 20, 28 and 36 dS/m) and a gradual decrease in relative growth of calli under NaCl salinity (1068.80- 29.39) was observed. The different crosses reflected significant difference in the terms of their relative growth and dry weight of calli. The best cross among the various crosses for determination of relative growth (631.98) was between Kh 375 x HD 2009 and for dry weight (21.8 mg/g) between Kh 375 x WH 147. The regeneration potential of non-selected and selected cell lines was assessed on MS medium fortified with 1.0 mg/l BAP and 0.2 mg/l IAA. The shoot regeneration of cell lines selected on various concentrations of NaCl (4, 8, 12, 20 and 28 dS/m) was obtained. The per cent calli forming shoots were decreased with increasing NaCl concentrations of all the tested crosses. However, whole plant regeneration was recorded from untreated calli of all the hybrids. Total 147 spikes obtained from various crosses provided a wide genetic base showing variations for spike length, spikelet number and grain number per spike. Hence, the investigation seems to be relevant for production of somaclones and will prove a beneficial research for development of salt tolerant genotypes in wheat in future.

Callus

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An alternative source for regenerable embryogenic callus induction from shoot tips of wheat [Triticum aestivum L.]

Indian Journal of Genetics and Plant Breeding (The) (2003)

Anju Sharma S. Uppal Sudhir Kumar Sharma Shalini Sharma

A reliable and efficient protocol for the regeneration of fertile plants derived from wheat [Triticum aestivum L.] shoot tips has been developed in present study. Shoot tips of 3, 4 and 5 days old seedlings of six wheat genotypes were cultured on T medium containing 10 mgll 2, 4-D for callus induction. Callus induction was significantly best in 3 days old shoot tips. Therefore, only 3 days old shoot tips were further used as a source of explant. Callus induction was significantly higher (82.9%) on T medium than 85 medium (76.3%) from 3 days old shoot tips. Callus initiation took place after 15 days on both the media. Regeneration took place through embryogenesis followed by shoot morphogenesis. Only 20 to 25 per cent shoot regeneration was obtained when both the calli (T and B5 medium derived) after 6 weeks of inoculation were transferred on MS medium without growth regulators or supplemented with 1 mgti SAP + 0.2 mg/l NAA. Kinetin at all levels (0 to 5 mg/l) also failed to induce shoot regeneration. However, 3 days old shoot tips when cultured on MS medium beatified with 2.5 mg/l 2, 4-D (MS1) or 2 mg/l 2, 4-D + 0.2 mg/l casein hydrolysate (MS2). Callus initiation took place within 6–7 days. Callus induction percentage was observed upto 94.4 on MS1 medium while 95.2 on MS2 medium. Shoot regeneration was achieved when calli were transferred after 5 weeks on MS medium bestified with 0.2 mgtl NAA. Regeneration percentage ranged from 75.2 (MS1 derived calli) to 83.3 (MS2 calli). Number of shoots per callus ranged from 3–8 and highest number was observed in WH 542. Regenerated plants were transferred to potted soil where normal growth stages proceeded with 75 per cent survival.

Callus

Kinetin

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Development of male-specific SCAR marker in date palm (Phoenix dactylifera L.)

Tree Genetics & Genomes (2013)

Charu Dhawan Pushpa Kharb Richa Sharma S. Uppal R. K. Aggarwal

Primer (cosmetics)

genomic DNA

Molecular marker

Bulked segregant analysis

Phoenix dactylifera

10.1007/s11295-013-0617-9

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Citations (50)

Genetic diversity analysis in date palm (Phoenix dactyliferaL.): a comparative assessment using ISSR and RAPD marker assays

The Journal of Horticultural Science and Biotechnology (2011)

Charu Mitra Pushpa Kharb S. Uppal Sunita Jain

SUMMARYA comparative study was conducted to evaluate genetic diversity in 45 genotypes of date palm (Phoenix dactylifera L.), including both male and female plants, employing RAPD and ISSR marker systems. The data were analysed to calculate the total number of bands, the number of polymorphic bands, the percentage polymorphism, the average number of bands per primer, the effective multiplex ratio (EMR), the polymorphic information content (PIC), the marker index (MI), and genetic similarity coefficients. The 37 RAPD and 53 ISSR primers used generated 363 and 608 scorable amplified products, respectively, of which 95.0% and 90.9% were polymorphic. The ISSR markers produced more information than the RAPD markers due to their higher EMR and MI values. Jaccard similarity values among male plants, female plants, and between all male and all female plants varied between 0.72 – 0.80. The results indicate the effectiveness of these two marker systems for demonstrating genetic relationships among date palm genotypes.

Phoenix dactylifera

Jaccard index

Molecular marker

Genetic similarity

Genetic relationship

10.1080/14620316.2011.11512780

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Citations (8)

Genetic divergence analysis in date palm (Phoenix dactylifera L.) genotypes using RAPD markers.

Haryana journal of horticultural sciences (2007)

Rekha Rani Pushpa Kharb S. Uppal Santosh Dhillon Parveen Batra

Phoenix dactylifera