Fernanda Edna Araújo Moura

Universidade Federal do Ceará

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Marilda Mendonça Siqueira

Fundação Oswaldo Cruz

Eduardo Antônio Gonçalves Ramos

University of Concepción

Leda Solano de Freitas Souza

Universidade Federal da Bahia

Rossana de Aguiar Cordeiro

Universidade Federal do Ceará

Leonardo Carletto Borges

Fundação Oswaldo Cruz

Felipe Serra Campelo

Universidade Federal do Ceará

Karine Sampaio Nunes Barroso

Hospital Universitário Walter Cantídio

Sandra Frabasile

Universidad de la República

Maria da Silva Pitombeira

Universidade Federal do Ceará

Lorena Tomé-Poderti

Institut Pasteur de Montevideo

Cooperative Institutions

Universidade Federal do Ceará

Hospital Universitário Walter Cantídio

Fundação Oswaldo Cruz

Universidade Estadual do Ceará

Universidade Federal da Bahia

Children's Specialized Hospital

Universidad de la República

Universidad La República

Hospital Ana Nery

Universidad de Montevideo

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Parainfluenza virus infections in a tropical city: clinical and epidemiological aspects

The Brazilian Journal of Infectious Diseases (2008)

Mariana Mota Moura Fé André Jalles Monteiro Fernanda Edna Araújo Moura

Little information on the epidemiology and clinical characteristics of human parainfluenza virus (HPIV) infections, especially in children from tropical countries, has been published. The aim of this study was to determine the frequency of HPIV infections in children attended at a large hospital in Fortaleza in Northeast Brazil, and describe seasonal patterns, clinical and epidemiological characteristics of these infections. From January 2001 to December 2006, a total of 3070 nasopharyngeal aspirates collected from children were screened by indirect immunofluorescence for human parainfluenza viruses 1, 2, and 3 (HPIV-1, 2 and 3) and other respiratory viruses. Viral antigens were identified in 933 samples and HPIV in 117. The frequency of HPIV-3, HPIV-1 and HPIV-2 was of 83.76%, 11.96% and 4.27%, respectively. Only HPIV-3 showed a seasonal occurrence, with most cases observed from September to November, and with an inverse relationship to the rainy season. Most HPIV-3 infections seen in outpatients were diagnosed as upper respiratory tract infections.

Human Parainfluenza Virus

10.1590/s1413-86702008000300006

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Citations (38)

Molecular methods for the diagnosis and characterization ofCryptococcus: a review

Canadian Journal of Microbiology (2010)

José Júlio Costa Sidrim Ana Karoline Freire Costa Rossana de Aguiar Cordeiro Raimunda Sâmia Nogueira Brilhante Fernanda Edna Araújo Moura

Cryptococcosis is a fungal infection caused by yeasts of the genus Cryptococcus , with Cryptococcus neoformans and Cryptococcus gattii as the primary pathogenic species. This disease is a threat to immunocompromised patients, especially those who have AIDS. However, the disease has also been described in healthy individuals. The tests used to identify these microorganisms have limitations that make final diagnosis difficult. However, currently there are specific gene sequences that can be used to detect C. neoformans and C. gattii from clinical specimens and cultures. These sequences can be used for identification, typing, and the study of population genetics. Among the main identification techniques are hybridization, which was the pioneer in molecular identification and development of specific probes for pathogen detection; PCR and other PCR-based methods, particularly nested PCR and multiplex PCR; and sequencing of specific genomic regions that are amplified through PCR, which is especially useful for diagnosis of cryptococcosis caused by unconventional Cryptococcus sp. Concerning microorganism typing, the following techniques have shown the best ability to differentiate between fungal serotypes and molecular types: PCR fingerprinting, PCR–RFLP, AFLP, and MLST. Thus, the accumulation of data generated by molecular methods can have a positive impact on monitoring resistant strains and treating diseases.

Cryptococcus gattii

Multilocus sequence typing

Multiplex

10.1139/w10-030

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Citations (49)

Respiratory syncytial virus infections during an epidemic period in Salvador, Brazil: viral antigenic group analysis and description of clinical and epidemiological aspects

Memórias do Instituto Oswaldo Cruz (2003)

Fernanda Edna Araújo Moura Leonardo Carletto Borges Silvana Augusta Rodrigues Portes Eduardo Antônio Gonçalves Ramos Marilda Mendonça Siqueira

Acute respiratory infections (ARI) caused by respiratory syncytial virus (RSV) were studied in 482 children from Salvador, BA, Brazil, over a period of 12 months. The epidemic period of RSV infections in Salvador occurred from February (summer) to August (winter), with peaks in May, June, and July. The grouping characteristics of 84 RSV present in nasopharyngeal secretions of children seen at a reference university hospital were analyzed. RSV represented 17.4% of all cases and 54.5% of the positive samples. Sixty-four RSV strains were assigned to group A and 14 to group B. Both groups circulated in the five months of the epidemic period studied. Infections by both groups of RSV were more frequent in children up to one year of age. The incidence of RSV ARI was slightly more frequent in males, although group B had more infected females.

Pneumovirus

10.1590/s0074-02762003000600005

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Infecções respiratórias agudas virais em crianças de Salvador: análise antigênica e genômica dos virus sincicial respiratório e adenovirus isolados com correlação clínica

Fernanda Edna Araújo Moura

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Protocolo de manejo de suspeita de infecção viral de vias respiratórias em pacientes com neoplasia hematológica do serviço de hematologia e transplante de medula óssea do Hospital Universitário Walter Cantídio (HUWC)

Revista de Medicina da UFC (2015)

Élida Lívia Rafael Dantas Luany Elvira Mesquita Carvalho Beatriz Araújo Maria da Silva Pitombeira Caroline Mary Gurgel Dias Florêncio

Infecção respiratória viral é causa significativa de morbi-mortalidade em paciente com doenças hematológicas. Deve ser suspeitado sempre que houver sintomas como coriza, obstrução nasal, odinofagia, tosse e dispnéia e deve-se seguir à identificação viral, quando possível. Os casos podem ser classificados como possível, provável ou confirmado. E observando-se o risco de progressão da infecção para vias aéreas inferiores, o diagnóstico deve ser precoce e as medidas terapêuticas específicas aos vírus bem como medidas de controle da infecção no ambiente hospitalar devem ser prontamente tomadas. O presente trabalho visa descrever um protocolo de manejo da infecção viral de vias respiratórias em pacientes com neoplasia hematológica dentro do Serviço de Hematologia e Transplante de medula do Hospital Universitário Walter Cantídio.

10.20513/2447-6595.2015v55n1p43-46

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Estudo de infecções respiratórias agudas virais em crianças atendidas em um centro pediátrico em Salvador (BA)

Jornal Brasileiro de Patologia e Medicina Laboratorial (2003)

Fernanda Edna Araújo Moura Leonardo Carletto Borges Leda Solano de Freitas Souza Dulce Helena Ribeiro Marilda Mendonça Siqueira

De janeiro a dezembro de 1998, foram analisados 482 casos de infecção respiratória aguda em crianças atendidas na emergência e nas enfermarias do Centro Pediátrico Professor Hosannah de Oliveira, da Universidade Federal da Bahia, em Salvador. O objetivo deste estudo é conhecer o papel dos vírus na etiologia destas infecções. Secreções nasofaríngeas foram examinadas para detecção viral por imunofluorescência e cultura celular. Vírus foram detectados em 154 casos (31,9%). O vírus sincicial respiratório (VSR) foi o mais prevalente (55,2%), seguido pelos vírus influenza A (17,5%) e parainfluenza 3 (16,2%), adenovírus (7,1%), influenza B (3,8%) e parainfluenza 1 (0,64%). O período epidêmico de VSR foi relacionado à estação de chuvas. Um surto de influenza foi observado nos meses de setembro a novembro. A maioria das infecções foi representada por infecção de trato respiratório superior (63,3%), enquanto que as infecções de trato respiratório inferior foram detectadas em 177 casos (36,7%). Infecções respiratórias agudas virais foram mais freqüentes em crianças até um ano de idade (72,7%).

10.1590/s1676-24442003000400003

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Citations (13)

DETECTION OF HUMAN METAPNEUMOVIRUS IN NASOPHARYNGEAL ASPIRATES OF BRAZILIAN CHILDREN BY IMMUNOFLUORESCENCE ASSAY

VIRUS Reviews & Research (2008)

Joyce Fonteles Ribeiro Fernanda Edna Araújo Moura

The circulation of human metapneumovirus is related to seasonal patterns of respiratory syncytial virus. The aim of this study was to determine the frequency of hMPV infection diagnosed by indirect immunofluorescence in children attending emergency department and general wards of Hospital Infantil Albert Sabin in Fortaleza, Ceara, northeastern Brazil, during the respiratory syncytial virus season in 2006. The analysis included 389 samples. Antigens of hPMV were detected in 39 samples. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.17

Immunofluorescence

Metapneumovirus

10.17525/vrr.v13i1-2.17

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Respiratory Infections in Children Associated to Parainfluenza Virus Type 4

Crister José Ocadaque Felipe Serra Campelo Fernanda Edna Araújo Moura

Little is known about the role of certain viruses, such as parainfluenza virus type 4 (HPIV-4) in the etiology of respiratory infections. The objective of this study is to present preliminary data on respiratory infections associated with HPIV-4 in children living in the city of Fortaleza capital of the state of Ceara, Brazil. A set of 846 samples of nasopharyngeal aspirates collected during four years from children with acute respiratory infections were submitted to direct immunofluorescence for the detection of HPIV-4. Fifteen HPIV-4 were detected. Cases of the upper respiratory infections, bronchiolitis and pneumonia were associated to this virus. Further studies are needed to clarify the clinical significance of HPIV-4 infections.

Human Parainfluenza Virus

Etiology

Respiratory infection

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The role of human bocavirus as an agent of community-acquired pneumonia in children under 5 years of age in Fortaleza, Ceará (Northeast Brazil)

Brazilian Journal of Microbiology (2022)

Felipe Serra Campelo Rossana de Aguiar Cordeiro Fernanda Edna Araújo Moura

Human bocavirus

10.1007/s42770-022-00806-1

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