Mamoru TOGASHI

Chugai Pharma (United States)

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Production of monozygotic twins by splitting of 2-cell stage embryos in mice.

Kachiku hanshokugaku zasshi (1987)

Mamoru TOGASHI Hiroshi Suzuki Tatsuya MIYAI Michio Okamoto

簡便でかつ効率良く一卵性双生仔を作出する目的で,マウスの2および4細胞期胚をピペッティングにより2分離し,それぞれの胚の胎仔への発生能について検討した。その結果,ピペッティングによって分離された胚は,透明帯を除去された状態で体外培養した場合でも高率に胚盤胞へ発生した。また,胚分離後の培養時間と移植時期(子宮日齢)との関係においては,1/2および2/4胚ともに48時間培養後,偽妊娠2日目の子宮角に移植した場合に最も高い胎仔生存率が得られた。実際に一卵性双生仔作出にあたっては1/2胚を対象として偽妊娠あるいは妊娠雌の子宮角への移植を行なった。その結果,偽妊娠雌への移植では1組,妊娠雌への移植では6組,計7組の一卵性双生仔マウスを得ることができた。以上のことから透明帯を除去したマウスの2および4細胞期からの分離胚は,体外培養を経た後でも胎仔への発生能力を十分保持していること,ならびに2細胞期胚からの一卵性双生仔の作出が可能であることが明らかとなった。

Monozygotic twin

10.1262/jrd1977.33.51

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Citations (22)

STATISTIC ANALYSIS ON QUANTITIVE EVALUATION OF FISH HABITAT IN THE RIVER BASED ON “NATIONAL CENSES ON RIVER ENVIRONMENT”

PROCEEDINGS OF HYDRAULIC ENGINEERING (2004)

Shinichi Mizuno Makoto SASAMOTO Shigeki Sakai Mamoru TOGASHI

In order to evaluate the habitat of the fish inhabiting the river, it is necessary to know the relation between the amount of habitations of a fish, and the physical characteristic of a habitat "National Censes on River Environment" contains information about plants and animals as well as the physical features of rivers in a certain format. In the present study, the dominant physical attributes forming a fish habitat in various rivers of the large area in Japan are discussed by using the above database.The population of fishes (for example Carassius gibelio langsdolfi) and physical characteristic in each river are approximated by a regression function which is derived from a multiple regression analysis. Contribution of the physical characteristic to the quantity of fishes in a different district is compared, and the feature for every area is described. And district feature is described by result from a principal component analysis. CPUE (Catch per Unit Effort) is proposed to standardize the amount of fishes capture and is used in analysis shown above.

Statistic

Fish habitat

10.2208/prohe.48.1573

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Effects of the motility of boar spermatozoa on the seperation using percoll density gradient centrifugation.

Nihon Yoton Gakkaishi (1989)

Kiyoshi Totsukawa Takeo KAYABA Yuuko OHSAWA Hiroshi Ueno Shinichiro Suto

Percoll

BOAR

Differential centrifugation

Density gradient

10.5938/youton.26.241

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Citations (0)

[Case of subserosal myoma of the uterus suspected as Meigs' syndrome].

PubMed (1967)

Mamoru TOGASHI Sanae Takizawa Saori Okada Hiroshi Hoshiai Yoji Moriyama

Meigs' syndrome

Source

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Citations (1)

A Method for Detecting the Optimum Day for Mating during 4-day Estrous Cycle in the Rat; Measuring the Value of Electrical Impedance of Vagina

EXPERIMENTAL ANIMALS (1987)

Masao Koto Masao Miwa Mamoru TOGASHI Koichiro Tsuji Michio Okamoto

A method for detecting the optimum day for mating in the rat was investigated. Cyclic changes of electrical impedance of vagina (EIV) were studied in the rats. EIV indicated high value (over 3,000 omega) only at proestrus, and it was lower (under 3,000 omega) at other stage of estrous cycle. These results apparently indicate that measuring the EIV made to distinguish proestrus from other phases of estrous cycle. The female caged with male showed high copulation rate (88 approximately 96%) when her EIV had been over 3,000 omega.

10.1538/expanim1978.36.2_195

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Citations (18)

Establishment of a New Hereditary-cataract Mouse (CSM) Strain Derived from SENCAR Mouse

EXPERIMENTAL ANIMALS (1988)

Tsuyoshi Sato Tatsuya MIYAI Isamu Akaike Mamoru TOGASHI MISAO KOJIMA

A mouse representing a new hereditary-cataract strain was found in a mouse colony and a new line was established strain CSM. These mice were investigated genetically, histologically and biochemically. The results suggested that this cataract was apparently inherited through two recessive autosomal genes. Histologically the denucleation process of lens fibers was abnormal and small vacuoles appeared in the equatorial region of the lens cortex at 12 days. Biochemically, insoluble protein and sodium increased in the lens with age.

Strain (injury)

10.1538/expanim1978.37.4_375

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Non-surgical Collection of Uterine Eggs in the Rat

EXPERIMENTAL ANIMALS (1980)

Yoshiro Ishijima Mamoru TOGASHI Shogo Kitajima

The non-surgical collection of rat embryos were examined. The apparatus for uterine flushing (Fig. 1 and 2) were designed to have a two-way system. Egg collection were made 5 days after mating. Eggs were collected from anesthetized donors using a pair of glass pipettes inserted to the end of each uterine horn through the cervix uteri. By non-surgical technique, it was successful to collect the eggs at an average of 4.0 from all the animal. The recovery rate was 40.7% as estimated the number of corpora lutea counted after the slaughter of the donor. The recovery rate in non-surgical methods was considerably lower as compared with that of the uterine flushing after the slaughter.

Uterine horns

Pipette

Flushing

Recovery rate

10.1538/expanim1978.29.3_355

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Separation and properties of boar semen using percoll density gradient centrifugation.

Nihon Yoton Gakkaishi (1988)

Kiyoshi Totsukawa Takeo KAYABA Hiroshi Ueno Shinichiro Suto Mamoru TOGASHI

Percoll

BOAR

Differential centrifugation

Density gradient

10.5938/youton.25.42

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Effects of recombinant human erythropoietin on haemolytic anaemia in mice

British Journal of Haematology (1990)

Osamu Cynshi Yasushi Shimonaka M. Higuchi Nobuo Imai Hiroshi Suzuki

Summary The effects of repeated administration of recombinant human erythropoietin (rHuEPO) were investigated in mice with haemolytic anaemia. Mice with haemolytic anaemia induced by phenylhydrazine (PHZ mice) were examined as an acute model and New Zealand black mice (NZB mice) at 13 months of age were examined as a chronic model. The plasma erythropoietin (EPO) level in PHZ mice was high and showed a strong inverse correlation with the Hb in the anaemia development period. However, it was relatively low in the recovery period from anaemia. On the other hand, the plasma EPO level in NZB mice showed a simple inverse correlation with the Hb. The rHuEPO was injected every day for a week into these mice. While a high plasma EPO level was maintained in PHZ mice, no significant effect was observed by injection with rHuEPO at dose of 600 IU/kg. However, in the recovery period from anaemia, RBC and haemoglobin in PHZ mice were increased by the rHuEPO treatment and recovered more quickly to their normal levels. In NZB mice, RBC and haemoglobin were also increased by treatment with rHuEPO at dose of 600 IU/kg. Anti‐RBC autoantibodies and anti‐EPO antibodies did not increase, while RBC and plasma EPO levels were increased by the rHuEPO treatment. These results suggest that some types of haemolytic anaemia are not always combined with high endogenous EPO levels and that exogenous rHuEPO may be effective for use in the treatment of haemolytic anaemia.

Reticulocyte

10.1111/j.1365-2141.1990.tb06377.x

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Citations (11)

The Development of Newborn C 57 BL/KsJ-dbm Mice Produced from Eggs Fertilized in Vitro

EXPERIMENTAL ANIMALS (1988)

Hiroshi Suzuki Saburo Shingai Mamoru TOGASHI Tatsuya MIYAI Yukage Motohide

The purpose of this study was to examine the development of newly born C57BL/KsJ-dbm mice produced from eggs fertilized in vitro. The embryos derived from fertilization in vitro (which was performed by using db/db eggs and adrenalectomized db/db (Adrex) spermatozoa, ) were transferred to the oviduct of MRL/MpJ pseudopregnant recipients 30 hr after insemination. 376 of these embryos yielded 65 young. Weight gain and urine glucose, plasma glucose and insulin levels were measured in these young as well as in Adrex males. The young produced by fertilization in vitro showed hyperglycemia, hyperinsulinemia and obesity. The physiological abnormalities in these young were similar to those in db/db young produced by natural mating between heterozygote (db/+) males and females. Adrex males did not show hyperglycemia but did show hyperinsulinemia. These results indicate that in vitro fertilization and embryo transfer is an effective means of producing fetuses or newborns with an overt genotype in genetically diabetic obese (db) mice.

10.1538/expanim1978.37.3_245

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Citations (2)