A A Kalugin

Institute of Biomedical Chemistry

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i-10 index

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E B Khoroshutina

Russian Academy of Sciences

НН

Н. Н. Соколов

Institute of Biomedical Chemistry

Michael A. Eldarov

Russian Academy of Sciences

Sergey Korolev

Saint Louis University

O T Samko

Institute of Biomedical Chemistry

КС

К.Г. Скрябин

Bioengineering Center

Vassilis Bouriotis

University of Crete

A B Fitsner

Maria Markaki

Aristotle University of Thessaloniki

ИВ

И. В. Карпычев

Timiryazev Institute of Plant Physiology

Cooperative Institutions

Russian Academy of Sciences

Institute of Biomedical Chemistry

FORTH Institute of Molecular Biology and Biotechnology

Bioengineering Center

University of Crete

Foundation for Research and Technology Hellas

Institute of Molecular Biology and Biotechnology

Academy of Medical Sciences

Federal Center Research Fundamentals of Biotechnology

Timiryazev Institute of Plant Physiology

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[Site-specific BcuAI endonuclease from Bacillus cereus A].

PubMed (1996)

Sergey Korolev O T Samko Michael A. Eldarov A A Kalugin E B Khoroshutina

A new restriction endonuclease was isolated from the Bacillus cereus BKM B-814 by means of the cell disruption with ultrasonication, ammonium sulfate fractionation of the cell-free extract, and chromatography on DEAE-Sepharose to give about 1400 U of the enzyme per gram of cells. The enzyme revealed the maximum activity at 30-37 degrees C, pH 7.6-8.2, and 5-10 mM MgCl2 under a high ionic strength (50 mM Tris-HCl, 100 mM NaCl). The site-specific endonuclease BcuAI was found to recognize the 5' G decreases G(A/T)CC sequence in double-stranded DNA and cleave it as shown with the arrow, thus being a true isoschisomer of the AvaII restriction endonuclease.

Sonication

Ammonium sulfate

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Isolation and characterization of CspBI, a novel NotI isoschizomer from Corynebacterium species B recognizing 5′‐GC↓GGCCGC‐3′

IUBMB Life (1998)

Nikolai N. Sokolov Michael A. Eldarov Mary Rina Sergey Korolev Maria Markaki

Abstract Sixty‐seven bacterial strains were surveyed for the presence of type II restriction endonucleases, especially concerning super‐rare‐cutting enzymes. Fourteen strains were found to contain specific enzymes. One of them CspBI from Corynebacterium species B was purified and characterized as an isoschizomer of NotI, which recognizes the palindromic octanucleotide sequence 5′‐GC↓GGCCGC‐3′ and cleaves at the position shown by the arrow. A comparison between the cleavage patterns on different DNAs, obtained with partially purified endonucleases from other detected producents including some strains of Corynebacterium, Cellulomonas and Rhizobium has shown that these enzymes do not belong to super‐rare‐cutting restriction endonucleases.

Isoschizomer

Corynebacterium

10.1080/15216549800201452

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[BsoAI--a new site specific endonuclease from Bacillus coagulans].

PubMed (1991)

Н. Н. Соколов Michael A. Eldarov N. V. Anikeicheva И. В. Карпычев O T Samko

A new site-specific endonuclease was detected in toluene lysates of Bacillus coagulans AUCM B-732 and designated as BcoAI. The enzyme was purified by fractionation of the cell-free extract in the two-phase PEG/dextran system followed by chromatography on DEAE-sepharose and phosphocellulose and shown to be free of nonspecific nucleases and phosphatases. BcoAI has three cleavage sites on lambda DNA, but does not cleave SV40, pBR322 and pUC19 DNA. BcoAI recognizes the sequence 5' CAC decreases GTG 3' on double-stranded DNA and cleaves it as indicated by the arrow to yield blunt-ended DNA fragments. Thus, BcoAI is a true isoschizomer of PmaCI from Pseudomonas maltophila C.

Bacillus coagulans

Isoschizomer

Cleavage (geology)

Cleave

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[Bsp153AI and BspM39I--new isoschizomers of PvuII restriction enzyme].

PubMed (1996)

A A Kalugin M. Rina Michael A. Eldarov В. Ф. Зарытова Maria Markaki

New restriction endonucleases, Bsp153AI and BspM39I, were isolated from Bacillus species strains 153A and M39, respectively. The enzymes recognize and cleave the nucleotide sequence [sequence: see text] and are true isoschizomers of restriction endonuclease PvuII.

Isoschizomer

Cleave

Recognition sequence

Homing endonuclease

Sequence (biology)

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[Site-specific endonucleases LplI and AagI].

PubMed (1990)

Н. Н. Соколов Z P Manialene V. Butkus A B Fitsner E B Khoroshutina

New site-specific endonucleases LplI and AagI have been isolated from the Lactobacillus plantarum and Achromobacter agile cells, respectively. The enzymes' purification stages included treatment of cell-free extracts with polyethylenimine, fractionation in two-phase system by Albertsson's method, chromatography on blue Sepharose and DEAE-cellulose. The results of cleavage of a 5'-32P-labelled oligodeoxynucleotide duplex by restriction endonucleases LplI and AagI indicate that these enzymes recognize and cut the sequence AT decreases CGAT, being therefore true isoschizomers of the ClaI restriction endonuclease from Caryophanon latum. The L. plantarum strain has 400 fold endonuclease productivity as compared with the ClaI producent and is perspective for preparative isolation of LplI.

Isoschizomer

Cyanogen bromide

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