Toshitsugu Kariya

Tokyo Teishin Hospital

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Takahiro Sakai

Yamaguchi University

Shoji Kume

Kansai Medical University

Yutaka Yatomi

University of Tokyo Hospital

Masaaki Higashihara

Kitasato University

Manabu YAMANAKA

Yokosuka Kyosai Hospital

Kyosuke Yamamoto

National Institute of Advanced Industrial Science and Technology

Yuki Matsumoto

Osaka University

Kyoya Takahata

Nagahama Institute of Bio-Science and Technology

Akira Tanabe

Renesas Electronics (Japan)

Hiroshi Oka

National Institute of Advanced Industrial Science and Technology

Cooperative Institutions

The University of Tokyo

Kyushu University

Saga Medical School Hospital

Saga University

Osaka University

Tohoku University

Teikyo University

Tokyo University of Science

Tokyo Teishin Hospital

University of Tokyo Hospital

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Characterization of Growth Hormone-Releasing Hormone Receptors in Pituitary Adenomas from Patients with Acromegaly*

The Journal of Clinical Endocrinology & Metabolism (1988)

Shoichiro Ikuyama SHOICHI NATORI Hajime Nawata Kenichi Kato Hiroshi Ibayashi

GHRH receptors in pituitary adenoma cell membranes from five patients with acromegaly were characterized using [125I] [His1,Nle27]GHRH-(1–32)NH2 ([125I]GHRHa) as a ligand. Specific binding of [125I]GHRHa to adenoma cell membranes was maximal within 20 min at 24 C, remained stable for 60 min, and was reversible in the presence of 500 nmol/L human GHRH-(1–44)NH2 (hGHRH). The specific binding increased linearly with 10–160 μg cell membrane protein. This binding was inhibited by 10−11–10−6 mol/L hGHRH in a dosedependent manner, with an ID50 of 0.20 nmol/L, but not by 10−7 mol/L vasoactive intestinal peptide, glucagon, somatostatin-14, somatostatin-28, TRH, LHRH, and CRH. The specific binding of [125I] GHRHa to the membranes was saturable, and Scatchard analysis of the data revealed an apparent single class of high affinity GHRH receptors in five adenomas from acromegalic patients; the mean dissociation constant was 0.30 ± 0.07 (±se) nmol/L, and the mean maximal binding capacity was 26.7 ± 7.0 (±se) fmol/mg protein. In three nonfunctioning pituitary adenomas, GHRH receptors were not detected. The plasma GH response to hGHRH (100 μg) injection was studied in four acromegalic patients before surgery. Plasma GH levels increased variably in response to hGHRH injection in all four patients. However, there was no correlation between the characteristics of the tumor GHRH receptors and plasma GH responsiveness in these patients. We conclude that pituitary GH-secreting adenomas have specific GHRH receptors. Exogenously administered GHRH presumably acts via these receptors, but the variations in plasma GH responsiveness to hGHRH in these patients cannot be directly related to the variations in binding characteristics of the GHRH receptors on the GH-secreting adenoma cells.

Growth hormone–releasing hormone

Dissociation constant

10.1210/jcem-66-6-1265

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Citations (24)

Effects of Thyroid-Stimulating Hormone on Human Thyroid Carcinoma and Adjacent Normal Tissue*

The Journal of Clinical Endocrinology & Metabolism (1978)

James B. Field Gail Bloom M. C. Chou Mary E. Kerins P. Reed Larsen

Effects of TSH on the adenylate cyclase-cAMP system and some parameters of intermediary metabolism were investigated in human thyroid carcinoma and adjacent normal thyroid tissue. Basal adenylate cyclase activity and cAMP concentrations were significantly higher in carcinomatous tissue. Basal [1-l4C]glucose oxidation, 32Pi incorporation into phospholipids, and organification of iodide were similar in both tissues. Stimulation of cAMP by TSH was significantly greater in normal compared to carcinomatous tissue. In neither tissue was there a good correlation between TSH stimulation of adenylate cyclase activity and cAMP concentrations. The TSH stimulation of 32Pi incorporation into phospholipids by TSH was significantly greater in normal tissue. The mean effect of TSH on iodide organification and glucose oxidation was similar in normal and carcinomatous tissue. Although specific binding of TSH was demonstrated in both normal and carcinomatous tissue, it did not correlate very well with stimulation of adenylate cyclase activity. Hormones other than TSH also augmented adenylate cyclase activity in two of the carcinomas. In individual patients, the relative responsivity of carcinomatous tissue compared to normal was not always consistent when all of the metabolic parameters were considered.

Organification

Basal (medicine)

10.1210/jcem-47-5-1052

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Citations (42)

HEMOLYTIC UREMIC SYNDROME IN AN ADULT

Nihon Naika Gakkai Zasshi (1972)

Satoshi Kimura Kenji Fujiwara Keiji Mitamura Toshitsugu Kariya Nishio Honda

Hemolytic uremic syndromeは, 上気道炎様症状および消化器症状の後, 急激な溶血性貧血, 急性腎不全, 赤血球形態異常, 血小板減少を来たす稀れな疾患で, その本態は, microangiopathic hemolytic anemiaと腎小動脈の血栓であるといわれているが, 現在迄の報告はほとんど小児に限られ, 成人例は極めて少ない. われわれは最近本症と思われる成人例を経験した. 患者は59才, 女. 上気道炎様症状, 消化器症状を呈した後, 貧血および乏尿をきたした. 血清GOT, LDH, bilirubinの一過性上昇, 赤血球寿命の短縮, burr cellの出現, reticulocytosis, 血小板減少, 骨髄のerythroid hyperplasiaなど全てmicroangiopathic hemolytic anemiaに一致した. BUNは187.5mg/dlまで上昇し, 急性腎不全の状態であつたが, 漸次回復し, 患者は3カ月後退院した. 以上の経過および検査成績から, 本症例は, 成人には稀れなhemolytic uremic syndromeであると考え報告した.

Reticulocytosis

Microangiopathic hemolytic anemia

Schistocyte

10.2169/naika.61.282

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A CASE OF SARCOIDOSIS ASSOCIATED WITH OCCLUSION OF AQUEDUCT OF MIDBRAIN AND INAPPROPRIATE SECRETION OF ADH SYNDROME

Nihon Naika Gakkai Zasshi (1967)

Yawara Yoshitoshi H Suzuki Yutaka UTSUMI Hiroshi Suzuki N Shimizu

サルコイドーシスの経過中,中脳水道閉塞によると思われる頭痛,悪心,嘔吐,めまい.難聴,記銘力・指南力低下などの症状を呈し, 副腎皮質ステロイド薬投与および髄液圧減圧術により,一時,小康をえたが,再び,記銘力・指南力低下とともに,低Na,低Cl血症,尿中Na, Cl排泄増加,高張尿などの所見を呈し,腎機能に異常なく,副腎機能不全もないことから, ADH異常分泌症候群と考えられた症例を報告する.本例の脳圧亢進症状,水電解質代謝異常は副腎皮質ステロイド薬が著効を示したことから,いずれもサルコイドーシスによると考えた.

10.2169/naika.56.1519

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Clinics, treatments of hemostasis abnormality and thrombotic diseases.Emergency treatments and primary cares of hemorrhagic diseases.

Medical practice (1994)

Toshitsugu Kariya

Abnormality

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Function of Intermediary Metabolism in Platelet Membrane (Proceedings of the 44 General Meeting of the Japan Hematological Society-2-) -- (Structure and Function of the Platelet Membrane)

日本血液学会雑誌 (1982)