Jörg Köhl

University of Lübeck

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Andreas Klos

Medizinische Hochschule Hannover

Christian M. Karsten

University of Lübeck

Yves Laumonnier

German Center for Lung Research

Wilfried Bautsch

Klinikum Braunschweig

W. Bautsch

Klinikum Braunschweig

Ralf J. Ludwig

University of Lübeck

Manoj K. Pandey

Cincinnati Children's Hospital Medical Center

Tillman Vollbrandt

University of Lübeck

Claudia Kemper

National Heart Lung and Blood Institute

Axel Kola

Charité - Universitätsmedizin Berlin

Cooperative Institutions

University of Lübeck

242

Medizinische Hochschule Hannover

139

Cincinnati Children's Hospital Medical Center

110

University Hospital Schleswig-Holstein

104

University of Cincinnati

Heidelberg University

Universität Hamburg

Ludwig-Maximilians-Universität München

University of Cincinnati Medical Center

Charité - Universitätsmedizin Berlin

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Chronic myelogenous leukemia‐derived basophilic granulocytes express a functional active receptor for the anaphylatoxin C3a

European Journal of Immunology (1993)

Titus Kretzschmar Andreas Jeromin Claudia Gietz Wilfried Bautsch Andreas Klos

Abstract The receptor for the inflammatory peptide C3 a has scarcely been examined on human cells. This work demonstrates that human tumor‐derived basophilic granulocytes express C3 a receptors, and presents parts of the hitherto unknown C3 a ‐signal transduction. When incubated with IL‐3, these cells specifically liberated histamine on C3a stimulation. Independent from IL‐3, 240000 ± 100000 receptors per cell with a K d of 5.6 ± 0.9 nM were determined. (Ca 2+ )j increased from 120 ± 35 nM to 300 ± 80 nM after a C3 a challenge, as measured by digital imaging fluorescence microscopy, and rested at its basal level in the presence of C3 a ‐desArg, the immediate catabolic product of C3 a in vivo. This (Ca 2+ ) i increase could be completely desensitized homologously by C3 a as well as inhibited by up to 75% by pertussis toxin. Thus, tumor‐derived basophils are suitable for cloning of the human C3 a receptor.

Basophilic

Chronic myelogenous leukemia

Anaphylatoxin

10.1002/eji.1830230239

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Citations (69)

An attempt to induce peripheral tolerance in a pig-to-primate transplantation model by infusion of ultrahigh numbers of donor peripheral blood mononuclear cells: first promising results

Transplantation Proceedings (2000)

André Simon Carsten Schröder Ulrich Martin M. G. Chikobava Christian Templin

Paraplegia

Thoracic aorta

10.1016/s0041-1345(00)01115-5

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Citations (1)

IL-4 Down-Regulates Anaphylatoxin Receptors in Monocytes and Dendritic Cells and Impairs Anaphylatoxin-Induced Migration In Vivo

The Journal of Immunology (2003)

Afsaneh Soruri Ziba Kiafard Claudia Dettmer J. Riggert Jörg Köhl

Abstract Anaphylatoxins mobilize leukocytes to the sites of inflammation. In the present study we investigated the impact of GM-CSF, IL-4, and IFN-γ on anaphylatoxin receptor expression in monocytes and dendritic cells (DC). IL-4 was identified as the strongest down-regulator of the receptors for C5a and C3a in monocytes and monocyte-derived DC (MoDC). To study the impact of IL-4 on anaphylatoxin-induced chemotaxis, an in vivo migration model was established. For this purpose, human monocytes and MoDC were injected i.v. into SCID mice that at the same time received anaphylatoxins into the peritoneal cavity. A peritoneal influx of human monocytes could be demonstrated by 4 h after injections of C5a and C3a. In line with receptor down-regulation, IL-4 treatment inhibited in vivo mobilization of human monocytes and MoDC in response to C5a and C3a. In addition to its effects on human cells, IL-4 reduced C5a receptors in murine bone marrow-derived DC and impaired recruitment of labeled bone marrow-derived DC in syngeneic BALB/c mice to i.p. injected C5a. Overall, these data suggest that inhibition of a rapid anaphylatoxin-induced mobilization of monocytes and DC to inflamed tissues represents an important anti-inflammatory activity of the Th2 cytokine IL-4.

Anaphylatoxin

Monocyte

10.4049/jimmunol.170.6.3306

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Citations (65)

C5L2-mediated signals control contraction of human Th1 responses

Molecular Immunology (2013)

Antonella F. Fara Jonathan Sumner Martin Kolev Ceri A. Roberts Knut Tore Lappegård

10.1016/j.molimm.2013.05.057

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Citations (0)

MODERN COMPLEMENT ANALYSIS - A DIAGNOSTIC TOOL IN TRAUMA, SIRS AND SEPSIS.

Shock (1997)

Jörg Köhl

Complement

10.1097/00024382-199703001-00287

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Autoimmune pre-disease

Autoimmunity Reviews (2022)

Katja Bieber Jennifer E. Hundt Xinhua Yu Marc Ehlers Frank Petersen

10.1016/j.autrev.2022.103236

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Citations (73)

Identification of Jagged1 as a novel ligand for CD46: An interaction required for normal induction and regulation of human TH1 responses

Immunobiology (2012)

Gaëlle Le Friec Devon Sheppard Christian M. Karsten Pat Whiteman Salley Al-Tilib Shamoun

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User Friendly

10.1016/j.imbio.2012.08.137

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Inhibitory Fcγ receptors negatively regulate C5aR-mediated effector functions in immune complex diseases

Molecular Immunology (2006)

Manoj K. Pandey Gabriele Köhl Jörg Köhl

Immune receptor

10.1016/j.molimm.2006.07.184

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Functional activities of synthetic anaphylatoxic peptides in widely used biological assays

Clinical & Experimental Immunology (1992)

Axel Kola Andreas Klos W. Bautsch Titus Kretzschmar Jörg Köhl

SUMMARY A comparison study was carried out between the modern ATP release assay (ARA) with guinea-pig platelets and the traditional guinea-pig ileum contraction assay (ICA). The biological activities of the anaphylatoxin C3a and synthetic C3a analogue peptides were determined in both assays. In dose-response curves with C3a, a human C3a peptide with the last 21 amino acids of the C terminus (C3a 56–77) and a peptide with 13 amino acids which was acylated N-terminal with the aromatic fluorenylmethoxycarbonyl group and an aminohexanoyl group (Fmoc-Ahx YRRGRAAALGLAR) were tested. The ARA turned out to be 100 times more sensitive than the ICA. In contrast to previous reports the 21 amino acid long C3a analogue peptide did not exhibit full C3a activity but only 7% (ARA) or 12% (ICA). The potentiation of biological activity in the ARA by coupling non-peptide acyl-residues N terminal to peptidic C3a analogues could be confirmed with Fmoc-Ahx-YRRGRAAALGLAR in the ICA. In addition, the tri-peptide Fmoc-Ahx-LAR displayed C3a specific activity in the ICA demonstrated by desensitization experiments.

Anaphylatoxin

Peptide Synthesis

10.1111/j.1365-2249.1992.tb03090.x

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Citations (11)

Novel roles for complement receptors in T cell regulation and beyond

Molecular Immunology (2013)

Claudia Kemper Jörg Köhl

Immune receptor

10.1016/j.molimm.2013.05.223

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Citations (64)