B.A. Didion

Minitube (United States)

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Randall S. Prather

University of Missouri

Jiude Mao

University of Missouri

Guangming Wu

Guangzhou Experimental Station

Tod C. McCauley

University of Arizona

Billy N. Day

University of Pittsburgh

S.L. Terlouw

United Animal Health (United States)

C. L. Markert

Northwestern University

T. C. Cantley

University of Missouri

J.R. Dobrinsky

Agricultural Research Service

C.N. Graves

University of Illinois Urbana-Champaign

Cooperative Institutions

University of Missouri

Minitube (United States)

The Graduate Center, CUNY

North Carolina State University

University of Wisconsin–Madison

Mississippi State University

Purdue University West Lafayette

Universidade Federal do Ceará

Universidad de Murcia

Washington University in St. Louis

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High Developmental Competence of Pig Oocytes after Meiotic Inhibition with a Specific M-Phase Promoting Factor Kinase Inhibitor, Butyrolactone I1

Biology of Reproduction (2002)

Guangming Wu Qing‐Yuan Sun Jiude Mao Liangxue Lai Tod C. McCauley

Butyrolactone I specifically inhibits M-phase promoting factor activation and prevents the resumption of meiosis. These experiments were conducted to examine effects of butyrolactone I on pig oocytes in a serum-free maturation system. The first experiment was conducted to determine the effect of butyrolactone I (0–100 μM) on nuclear maturation. At concentrations of ≥12.5 μM, germinal vesicle breakdown was prevented in >90% of the oocytes after 24 h of culture. In the second experiment, the kinetics of in vitro maturation of butyrolactone I-treated oocytes was investigated. Oocytes were treated with 0 or 12.5 μM butyrolactone I and FSH for 20 h and then cultured with LH in the absence of butyrolactone I for another 24 h. Fewer butyrolactone I-treated oocytes reached MII stage at 36 h compared with controls (5.8% vs. 62.4%, P < 0.01). However, by 44 h, 83.4% of butyrolactone I-treated oocytes reached MII compared with 88.6% of controls. In the third experiment, butyrolactone I-treated oocytes were fertilized and cultured in vitro. No differences (P > 0.05) were found between controls and treated groups in cleavage rate, blastocyst rate, or mean number of cells per blastocyst. Effects of butyrolactone I on mitogen-activated protein kinase activation and localization of microfilaments and active mitochondria were examined by Western blot analysis and laser scanning confocal microscopy, respectively. The results suggested that although butyrolactone I reversibly inhibited germinal vesicle breakdown and mitogen-activated protein kinase activation, it did not affect mitochondrial and microfilament dynamics. Butyrolactone I is a potent inhibitor of nuclear maturation of porcine oocytes, and the inhibition is fully reversible.

Germinal vesicle

10.1095/biolreprod67.1.170

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Citations (71)

Staining procedure to detect viability and the true acrosome reaction in spermatozoa of various species

Gamete Research (1989)

B.A. Didion J.R. Dobrinsky James R. Giles C.N. Graves

Abstract A simple dual stain procedure (DS) for simultaneously determining sperm viability and acrosomal status is described. The DS includes the use of the vital stain trypan blue to detect live and dead spermatozoa and Giemsa to detect the presence or absence of an acrosome. For staining, spermatozoa are washed, incubated with trypan blue, washed, dried onto slides, and subjected to Giemsa. Dead spermatozoa stain blue in the postacrosomal region while live spermatozoa remain unstained. The acrosome stains light purple–dark pink while acrosome‐free sperm remain unstained. This staining pattern enables differentiation of spermatozoa which have undergone a true acrosome reaction (TAR) from those which have undergone a false acrosome reaction (FAR). Incubation of bull, boar, ram, and stallion spermatozoa for 60 minutes at 37°C in the presence of calcium ionophore A23187 increased the proportion of spermatozoa undergoing a TAR in all species except the stallion. Incubation of bull spermatozoa for up to 24 hours at 37°C resulted in a decrease over time in the percentage of live acrosome‐intact spermatozoa and a simultaneous increase in the percentage of spermatozoa categorized as having undergone a TAR and FAR. The DS could be a useful technique in evaluating sperm viability and acrosomal status in fertilization and clinical studies.

Acrosome reaction

10.1002/mrd.1120220106

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Citations (159)

Rapid communication: Genetic linkage and physical mapping of a porcine sequence characterized amplified region (SCAR) to chromosome 6.

Journal of Animal Science (2000)

B.A. Didion A. A. Paszek G. A. Rohrer H. Sunny Sun Christopher K. Tuggle

Journal Article Rapid communication: Genetic linkage and physical mapping of a porcine sequence characterized amplified region (SCAR) to chromosome 6 Get access B. A. Didion, B. A. Didion *DEKALB, DeKalb, IL 60015, USA 1Correspondence: phone: 636-737-5655; fax: 636-737-7002; E-mail: bradley.a.didion@monsanto.com. Present address: Monsanto, 700 Chesterfield Parkway North, St. Louis, MO 63198. Search for other works by this author on: Oxford Academic PubMed Google Scholar A. A. Paszek, A. A. Paszek *DEKALB, DeKalb, IL 60015, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar G. A. Rohrer, G. A. Rohrer †U.S. Meat Animal Research Center, USDA-ARS, Clay Center, NE 68933-0166 Search for other works by this author on: Oxford Academic PubMed Google Scholar H. S. Sun, H. S. Sun ‡Department of Animal Science, Iowa State University, Ames 50011 Search for other works by this author on: Oxford Academic PubMed Google Scholar C. K. Tuggle C. K. Tuggle ‡Department of Animal Science, Iowa State University, Ames 50011 Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Animal Science, Volume 78, Issue 12, December 2000, Pages 3195–3196, https://doi.org/10.2527/2000.78123195x Published: 01 December 2000 Article history Received: 22 October 1999 Accepted: 10 August 2000 Published: 01 December 2000

10.2527/2000.78123195x

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Citations (3)

Constructing cDNA Libraries with Fewer Clones that Contain Long poly(dA) Tails

BioTechniques (2001)

Honglin Jiang Nathan J. Bivens Jim Ries Kristin M. Whitworth Jonathan A. Green

Columbia university

10.2144/01311bm05

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Citations (14)

Parthenogenetic activation of mouse and pig oocytes matured in vitro

Theriogenology (1990)

B.A. Didion Michael J. Martin C. L. Markert

Germinal vesicle

Parthenogenesis

In vitro maturation

10.1016/0093-691x(90)90035-r

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Citations (27)

259 A RAPID, NON-PCR-BASED BOVINE EMBRYO BIOPSY SEXING ASSAY

Reproduction Fertility and Development (2008)

Reiner Bleher W.T. Erwin Ann Marie Paprocki C. M. Syverson R. Koppang

Sex determination of bovine embryos in conjunction with embryo transfer is an established method to maximize efficiency by producing offspring with the preferred gender. Present methodology for sexing embryos is based on PCR, which requires molecular laboratory equipment, trained personnel, and several hours before a result becomes known. We developed a simple, rapid, non-PCR procedure for identification of Y-chromosomes in bovine blastomeres recovered via biopsy. A biopsy (n = 5 to 8 blastomeres) was taken from a blastocyst-stage embryo and transferred onto a plastic slide. After drying and fixation, the cells were denatured and incubated with a peptide nucleic acid probe designed to target unique Y-chromosome specific sequence. The probe was conjugated to a fluorescent dye (CY-3) which enables Y-chromosome detection as a bright spot within blastomere nuclei when using a fluorescent microscope. The absence of signal indicates female embryonic DNA. From placement of the biopsy onto a plastic slide, the methodology required approximately 75 minutes to determine embryo gender. The accuracy of the biopsy sexing procedure was demonstrated by parallel gender determination of the same embryo using an established PCR method designed for the bovine amelogenin locus. Based on 18 in vitro-produced bovine embryos generating a result for both assays, there was a 94.4% match (17/18) of gender assignment. The present technology represents a simple alternative to PCR-based embryo sexing technology. Research is ongoing for future development of live embryo sexing determination.

Sexing

Blastomere

10.1071/rdv21n1ab259

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Citations (5)

149 LAPAROSCOPIC EMBRYO TRANSFER IN PIGS

Reproduction Fertility and Development (2008)

S.L. Terlouw Ewerling Sonja B.A. Didion J.R. Dobrinsky

In an effort to optimize the number of live offspring from cloned and transgenic pig embryos, embryos are surgically transferred into the isthmus region of the oviduct soon after micromanipulation. Surgical embryo transfer in pigs is successful but still an invasive process. Laparoscopic embryo transfer (Besenfelder et al. 1997 Theriogenology 47, 1051–1060) is much less invasive than surgery and is more adaptable to a variety of commercial embryo transfer conditions. Our goal was to develop the use the laparoscope as an alternative method of embryo transfer for micromanipulated embryos. Naturally cycling maternal white line donor and recipient females were used for laparoscopic embryo transfer. Donors were selected to be in estrus 0 to 24 h before recipients. Two- to four-cell embryos were surgically recovered via mid-ventral laparotomy and immediately prepared for laparoscopic transfer into the oviduct through the infundibulum or through puncture of the oviduct into the ampulla. Sixty-nine embryos (range, 13–20) were transferred into the oviduct via the infundibulum of four recipients. Two recipients farrowed (50%), one recipient spontaneously aborted on Day 27, and one returned to estrus on Day 25 of the estrous cycle. Twenty-one pigs were born (10.5 pigs/farrowed sow) resulting in 30% (21/69) of transferred embryos becoming live offspring. Ninety-seven embryos (range, 10-21) were transferred into the ampulla of the oviduct of six recipients. Four recipients farrowed (67%) and 2 returned to estrus on Day 20 and Day 28. Thirty-nine pigs were born (9.5/sow farrowed) resulting in 39% (38/97) of transferred embryos becoming live offspring. Observations indicate that it is much easier to find the oviduct and deliver embryos via puncture into the ampulla than find and insert the transfer catheter into the infundibulum. Using the efficiencies generated from these data, 1.1 more pigs can be expected per transfer by transferring embryos to the oviduct via puncture than into the oviduct via the infundibulum. These data demonstrate the effective use of the laparoscope for oviductal embryo transfer. Further work is needed to determine if the laparoscope improves the production of live offspring compared to surgical embryo transfer.

Oviduct

Infundibulum

Ampulla

10.1071/rdv20n1ab149

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Citations (1)

Chromosomal abnormalities in Day-6, in vitro-produced pig embryos

Theriogenology (2003)

Tod C. McCauley M.R. Mazza B.A. Didion Jiude Mao Guangming Wu

Polyploid

Mitotic index

10.1016/s0093-691x(03)00172-9

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Citations (52)

Effect of epidermal growth factor and insulin-like growth factor I on porcine preantral follicular growth, antrum formation, and stimulation of granulosal cell proliferation and suppression of apoptosis in vitro1

Journal of Animal Science (2004)

Jiude Mao Michael F. Smith Edmund B. Rucker Guangming Wu Tod C. McCauley

The object of this study was to investigate the role of epidermal growth factor (EGF) and IGF-I in the regulation of preantral follicular growth, antrum formation, and granulosal cell proliferation/apoptosis. Porcine preantral follicles were manually dissected and cultured for up to 8 d in Waymouth's (Exp. 1) or α-minimum Eagle's essential medium (Exp. 2 and 3) supplemented with 10 μg/mL of transferrin, 100 μg/mL of L-ascorbic acid, and 2 mU/mL of ovine FSH, in the presence (Exp. 1 and 3) or absence (Exp. 2) of 7.5% fetal calf serum. According to the experimental protocol, IGF-I (0, 1, 10, or 100 ng/mL; Exp. 1), or IGF-I (50 ng/mL), EGF (10 ng/mL) and EGF+IGF-I (Exp. 2 and 3) were added to the culture media. In Exp. 1, follicles exhibited a concentration-dependent response (P < 0.05) to IGF-I, with the highest rates of granulosal cell proliferation, follicular integrity, and recovery rate of cumulus cell-oocyte complexes and lowest incidence of apoptosis occurring at the highest IGF-I dose. In Exp. 2 serum-free medium, granulosal cell proliferation was low (1 to 5%), irrespective of whether EGF and/or IGF-I were present and cellular apoptosis was increased (P < 0.05) on d 4 and 8 in the EGF+IGF-I group compared with the addition of either factor alone. In Exp. 3, granulosal cell proliferation was high in all follicles cultured in serum-containing medium for the first 3 d, but fell sharply (P < 0.05) on d 4, except in media containing IGF-I. Collectively, EGF and IGF-I increased granulosal cell proliferation, decreased apoptosis, and promoted follicular antrum formation. These results may provide useful information for developing a preantral follicular culture system in which the oocytes are capable of fertilization and embryonic development.

Antral follicle

10.2527/2004.8271967x

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Citations (120)

Acrosomal status of boar sperm 1 day after collection and transport

Theriogenology (2008)

T. Schlimgen W. Bertrand Reiner Bleher S.L. Terlouw B.A. Didion

Spermatozoon

BOAR

10.1016/j.theriogenology.2008.06.050

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Citations (0)