Bruce A. Webb

University of Kentucky

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Jeremy A. Kroemer

Bayer (United States)

Douglas L. Dahlman

University of Kentucky

Matthew W. Turnbull

Clemson University

Michel Cusson

Natural Resources Canada

Kent S. Shelby

Biological Control of Insects Research Laboratory

Liwang Cui

University of South Florida

Angelika Fath‐Goodin

ParaTechs (United States)

Anne‐Nathalie Volkoff

Université de Montpellier

Lynn M. Riddiford

University of Washington

Don Stoltz

Brigham and Women's Hospital

Cooperative Institutions

University of Kentucky

Centre National de la Recherche Scientifique

Université de Montpellier

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Université de Tours

Institut National de la Recherche Agronomique

University of Washington

Inserm

University of Bern

Agricultural Research Service

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Stimulation of polydnavirus replication by 20-hydroxyecdysone

Experientia (1992)

Bruce A. Webb M D Summers

Ecdysteroid

Southern blot

10.1007/bf01919157

Cite

Citations (49)

Relationships between polydnavirus gene expression and host range of the parasitoid wasp Campoletis sonorensis

Journal of Insect Physiology (2000)

Liwang Cui Ana I. Soldevila Bruce A. Webb

Permissive

Hemolymph

Heliothis virescens

Autographa californica

Parasitoid wasp

Helicoverpa zea

10.1016/s0022-1910(00)00059-7

Cite

Citations (58)

Metal ion binding to apo, holo, and reconstituted horse spleen ferritin

Journal of Inorganic Biochemistry (1995)

S. Pead E. Durrant Bruce A. Webb Christopher A. Larsen Duane E. Heaton

Stoichiometry

10.1016/0162-0134(94)00050-k

Cite

Citations (66)

Shared and species-specific features among ichnovirus genomes

Virology (2007)

Kohjiro Tanaka Renée Lapointe Walter E. Barney Andrea M. Makkay Don Stoltz

10.1016/j.virol.2006.11.034

Cite

Citations (88)

A supracellular system of actin-lined canals controls biogenesis and release of virulence factors in parasitoid venom glands

Journal of Experimental Biology (2009)

Roberto Ferrarese Jorge Morales Daniel Fimiarz Bruce A. Webb Shubha Govind

Parasitoid wasps produce virulence factors that bear significant resemblance to viruses and have the ability to block host defense responses. The function of these virulence factors, produced predominantly in wasp venom glands, and the ways in which they interfere with host development and physiology remain mysterious. Here, we report the discovery of a specialized system of canals in venom glands of five parasitoid wasps that differ in their infection strategies. This supracellular canal system is made up of individual secretory units, one per secretory cell. Individual units merge into the canal lumen. The membrane surface of the proximal end of each canal within the secretory cell assumes brush border morphology, lined with bundles of F-actin. Systemic administration of cytochalasin D compromises the integrity of the secretory unit. We show a dynamic and continuous association of p40, a protein of virus-like particles from a Drosophila parasitoid, L. heterotoma, with the canal and venom gland lumen. Similar structures in three Leptopilina species and Ganaspis xanthopoda, parasitoids of Drosophila spp., and Campoletis sonorenesis, a parasitoid of Heliothis virescens, suggest that this novel supracellular canal system is likely to be a common trait of parasitoid venom glands that is essential for efficient biogenesis and delivery of virulence factors.

Heliothis virescens

10.1242/jeb.025718

Cite

Citations (32)

Characterization of Campoletis sonorensis ichnovirus segment I genes as members of the repeat element gene family

Journal of General Virology (2002)

Roland S. Hilgarth Bruce A. Webb

Campoletis sonorensis ichnovirus ( Cs IV) is a symbiotic virus associated with the endoparasitic wasp C . sonorensis . The virus is injected into the wasp’s host, Heliothis virescens , during oviposition. One Cs IV gene has been identified as a repeat element (rep) gene and encodes a ubiquitous imperfectly conserved 540 bp sequence. We report the sequencing and mapping of a rep-containing segment, segment I, that hybridizes to a known rep sequence from segment O 1 . Analysis of this 8·6 kbp segment identified three ORFs having high similarity to the 540 bp rep sequence. All three rep sequence ORFs were expressed in parasitized H . virescens as well as in C . sonorensis tissues. Two of these rep genes, I 0.9 and I 1.1, have single copies of the 540 bp repeat sequence, while the third rep gene, I 1.2, has two imperfect copies, which are more similar to each other than to sequences on the segment I single-motif genes. Like the Cs IV BHv 0.9 rep gene, the segment I rep genes lack introns and a signal peptide, suggesting that they are not secreted. Based on their similarity in nucleotide sequence, predicted amino acid sequence and gene structure, the three segment I repeat-containing genes, I 0.9, I 1.1 and I 1.2, are new members of the rep gene family.

10.1099/0022-1317-83-10-2393

Cite

Citations (21)

Iron binding proteins and their roles in the tobacco hornworm,Manduca sexta (L.)

Journal of Comparative Physiology B (1988)

Helmut A. Huebers E Huebers C. A. Finch Bruce A. Webb JW Truman

Hemolymph

Manduca

Sphingidae

10.1007/bf00695327

Cite

Citations (98)

Promoter analysis of a cysteine-rich Campoletis sonorensis polydnavirus gene.

Journal of General Virology (1997)

L Cuit Bruce A. Webb

Promoter activity of the Campoletis sonorensis polydnavirus (CsPDV) WHv1.6 gene was analysed by transient transfection assays in insect cell culture using constructs expressing the CAT gene. Deletions of the WHv1.6 gene promoter were used to define promoter regions important for expression. Progressive deletion of the regions upstream of the TATA box reduced the promoter activity, whereas deletions eliminating the TATA box abolished promoter activity. Cis-activating elements were detected up to 1 kb upstream of the WHv1.6 transcription initiation site (TIS). Promoter elements increasing transcription were detected between 444 and 550 bp and between 831 and 1035 bp relative to the TIS. Analysis of the 3′ flanking sequences of the WHv1.6 gene indicated that the polyadenylation signals were the only important elements affecting expression in the constructs. Comparison of promoter regions of four cysteine-rich CsPDV genes revealed homologous sequences that may be important for transcriptional regulation of polydnavirus gene expression in parasitized Heliothis virescens larvae.

TATA box

Transcription

10.1099/0022-1317-78-7-1807

Cite

Citations (13)

Expression of polydnavirus genes under polydnavirus promoter regulation in insect larvae infected with baculovirus recombinants

Journal of General Virology (1996)

Ana I. Soldevila Bruce A. Webb

We have evaluated the use of baculoviruses to deliver Campoletis sonorensis polydnavirus (CsPDV) genomic DNA into lepidopteran larvae to facilitate the identification of functional CsPDV genes. Genomic fragments consisting of regulatory (promoter) and coding sequences for two CsPDV genes (VHv1.1 and WHv1.6) were used to generate CsPDV-baculovirus recombinants and evaluate the expression of genes under the regulation of the CsPDV promoters. Northern blot and primer extension studies established that CsPDV genes were expressed under the control of their own promoters in these CsPDV-baculovirus recombinants. Transcripts were detected as early as 4 h post-infection indicating that temporal activity of CsPDV promoters was retained. The VHv1.1 gene product as expressed from CsPDV-baculovirus recombinants was identical in size and in functional properties to that produced in CsPDV-infected insects. CsPDV-baculovirus recombinants may be useful for the screening and characterization of polydnavirus genes with functional activities that can only be evaluated in insect larvae.

Primer extension

genomic DNA

10.1099/0022-1317-77-7-1379

Cite

Citations (12)

Genomic and Morphological Features of a Banchine Polydnavirus: Comparison with Bracoviruses and Ichnoviruses

Journal of Virology (2007)

Renée Lapointe Kohjiro Tanaka Walter E. Barney James B. Whitfield Jonathan C. Banks

Many ichneumonid and braconid endoparasitoids inject a polydnavirus (PDV) into their caterpillar hosts during oviposition. The viral entities carried by wasps of these families are referred to as "ichnoviruses" (IVs) and "bracoviruses" (BVs), respectively. All IV genomes characterized to date are found in wasps of the subfamily Campopleginae; consequently, little is known about PDVs found in wasps of the subfamily Banchinae, the only other ichneumonid taxon thus far shown to carry these viruses. Here we report on the genome sequence and virion morphology of a PDV carried by the banchine parasitoid Glypta fumiferanae. With an aggregate genome size of approximately 290 kb and 105 genome segments, this virus displays a degree of genome segmentation far greater than that reported for BVs or IVs. The size range of its genome segments is also lower than those in the latter two groups. As reported for other PDVs, the predicted open reading frames of this virus cluster into gene families, including the protein tyrosine phosphatase (PTP) and viral ankyrin (ank) families, but phylogenetic analysis indicates that ank genes of the G. fumiferanae virus are not embedded within the IV lineage, while its PTPs and those of BVs form distinct clusters. The banchine PDV genome also encodes a novel family of NTPase-like proteins displaying a pox-D5 domain. The unique genomic features of the first banchine virus examined, along with the morphological singularities of its virions (IV-like nucleocapsids, but enveloped in groups like some of the BVs), suggest that they could have an origin distinct from those of IVs and BVs.

Subfamily

Genomic Organization

Ankyrin repeat

Genome size

10.1128/jvi.02702-06

Cite

Citations (94)