N. R. Kale

Savitribai Phule Pune University

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Shobhana V. Bhide

Savitribai Phule Pune University

Meena Karve

Institute of Bioinformatics

N. B. Patil

Shivaji University

Raymond L. Edwards

University of Bradford

Yasmin Y. Talib

Savitribai Phule Pune University

A. Bhati

Dr. Bhim Rao Ambedkar University

Nima D. Kamath

Savitribai Phule Pune University

Sushma Y. Sawhney

Savitribai Phule Pune University

Chandni Desai

Madhavi Chaudhari

Savitribai Phule Pune University

Cooperative Institutions

Savitribai Phule Pune University

University of Bradford

Oxford University Press (United Kingdom)

Louisiana State University Agricultural Center

Louisiana State University

University of Oxford

Massachusetts Institute of Technology

Brandeis University

Institute of Bioinformatics

Sardar Patel University

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Separation of Starch Components by Affinity Chromatography

ACS symposium series (1981)

Meena Karve Shobhana V. Bhide N. R. Kale

We have observed that proteins in solution display strong hydrophobic interaction with amylose-iodinecomplex, forming insoluble precipitates which can be dissociated by reduction of iodine with a suitable reducing agent (16). Based on this observation we have developed a simple method for the separation of starch components by affinity chromatography on crosslinked gelatin granules in acetate buffer (pH 4.8, 0.1 M) containing urea (2 M) + Iodine (7.87 x 10-4M) + KI (1.21 x 10-2M) + SDS (1.0 x 10-3M). The amylose-SDS-iodine -complex is retained on the column and the amylopectin-SDS-iodine-complex appears In the effluent. The column is washed free of amylopectin-SDS-iodine-complex with the same solution and the amylose-SDS-iodine-complex was eluted with acetate buffer (pH 4.8, 0.1 M) containing urea (8 M) + SDS (1.0 x 10-3M). The amylose and amylopectin fractions were characterized by determining their blue value and β-amylolysis limit. A spectrophotometric method for the determination of iodine binding capacity of amylose, amylopectin and starch on a micro-scale is developed.

10.1021/bk-1981-0150.ch037

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Citations (3)

The structure of xylindein

Tetrahedron (1965)

Raymond L. Edwards N. R. Kale

Spectral Analysis

10.1016/s0040-4020(01)98346-2

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Citations (43)

Turbidimetric assay of the acidic polysaccharides in agar

Analytica Chimica Acta (1974)

N. B. Patil N. R. Kale

10.1016/0003-2670(74)80033-4

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Constituents of the higher fungi. Part IV. Involutin, a diphenylcyclopenteneone from Paxillus involutus(Oeder ex Fries)

Journal of the Chemical Society C Organic (1967)

Raymond L. Edwards G. C. Elsworthy N. R. Kale

The isolation of 5-(3′,4′-dihydroxyphenyl)-3,4-dihydroxy-2-(p-hydroxyphenyl)cyclopent-2-ene-1-one from Paxillus involutus(Oeder ex Fries) is described. Properties and reactions of this compound leading to the assignment of its structure are described. The properties of this compound are compared with those of a related synthetic compound.

Paxillus involutus

10.1039/j39670000405

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Citations (21)

A Simple Preparative Method for the Isolation of Amylose and Amylopectin from Potato Starch

Preparative Biochemistry (1988)

Yasmin Y. Talib Meena Karve Shobhana V. Bhide N. R. Kale

Abstract The defatted starch was dispersed in NaOH (1 M) and neutralized with HCl (1 M). The amylose 1-butanol complex is adsorbed on defatted cellulose powder in the solvent system containing acetate buffer (pH 4.8, 0.1 M) ± urea (2 M) ± 1-butanol (8.5 %, v/v). The complex adsorbed on cellulose powder is separated by centrifugation (2418 g). The sediment is washed with the solvent system-I to obtain the intermediate fraction. The adsorbed amylose is eluted with urea (2 M) in acetate buffer (pH 4.8, 0.1 M). The amylose, intermediate fraction and amylopectin were precipitated with ethanol, washed free of urea and air dried. They were characterized by determining their blue value and β -amylolysis limit.

Isolation

Potato starch

10.1080/00327488808062521

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The Interaction of Sodium Dodecyl Sulfate, a Competing Ligand, with Iodine Complexes of Amylose and Amylopectin

ACS symposium series (1981)

Shobhana V. Bhide Meena Karve N. R. Kale

Amylose is a unique polysaccharide which forms a helical blue-coloured complex with iodine (I-3). It also forms helical complexes with a variety of organic compounds such as 1-butanol, 1-pentanol, cyclohexanol, SDS etc. The interaction of SDS, a competing ligand with iodine (I-3) complexes of amylose and amylopectin is studied spectrophotometrically. It is observed that the reduction in absorbance at 640 nm accompanied by the blue shift (640-570 nm) in the absorption spectrum is governed by the sequence of addition of the reagents, implying that this interaction is closely associated with the coil→helix transition of the polymer chain. Perturbation of this complex with sodium thiosulphate and urea has revealed that the transition from helix→coil is rather sluggish and hydrophobic interactions play an important role in the stability of this complex.

Sodium dodecyl sulfate

Hyperchromicity

Absorbance

10.1021/bk-1981-0150.ch033

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Citations (6)

Isolation and characterization of high-molecular-weight amylose by dispersion of starch granules with urea

Biochemical Journal (1972)

N. B. Patil N. R. Kale

Research Article| June 01 1972 Isolation and characterization of high-molecular-weight amylose by dispersion of starch granules with urea N B Patil; N B Patil Search for other works by this author on: This Site PubMed Google Scholar N R Kale N R Kale Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1972) 128 (1): 61P. https://doi.org/10.1042/bj1280061Pa Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation N B Patil, N R Kale; Isolation and characterization of high-molecular-weight amylose by dispersion of starch granules with urea. Biochem J 1 June 1972; 128 (1): 61P. doi: https://doi.org/10.1042/bj1280061Pa Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1972 London: The Biochemical Society1972 Article PDF first page preview Close Modal You do not currently have access to this content.

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10.1042/bj1280061pa

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Citations (1)

A Modified Method for the Preparation of Agarose

Preparative Biochemistry (1977)

Meena Karve N. R. Kale

Abstract A simple procedure for the preparation of agarose suitable for electrophoresis is developed in which anionic polysaccharides are removed by extracting the agar gel-granules with phosphate buffer (0.03 M, pH 6.8)containing urea (4 M), followed by electrophoresis in the same buffer system. Further, alkali treatment in the presence of sodium borohydride, eliminates electroendosmosis, giving essentially a neutral agarose, as Judged by the electrophoretic behaviour of basic substances like crystal violet and cytochrome C. The purified agarose with yields 60–65%, has a sulphur content less than 0.1%, and forms rigid, transparent gels.

Agarose

10.1080/00327487708061657

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Citations (1)

Ligand-induced conformational changes in amylose, studied by spectrophotometric and enzymic methods

Biochemical Journal (1972)

Shobhana V. Bhide N. R. Kale

10.1042/bj1280060pc

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Citations (1)

Preparation of Crosslinked Starch Beads as a Medium for Gel Filtration

Starch - Stärke (1989)

Madhavi Chaudhari Nima D. Kamath Shobhana V. Bhide N. R. Kale

Abstract Potato starch is dispersed in sodiumhydroxide and fine emulsion is obtained by stirring in liquid paraffin at 80°C. The emulsion is crosslinked with epichlorohydrin in presence of sodium borohydride to obtain thermostable beads. Beads of uniform size were obtained by sedimentation under gravity. Mixtures of proteins such as bovine serum albumin (BSA) and cytochrome c can be separated on columns (Ø 2.5 × 25.0 cm) packed with these beads. The elution profiles are similar to that of Sephadex G‐100.

Epichlorohydrin

Sephadex

Filtration (mathematics)

Bovine serum albumin

10.1002/star.19890411103

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Citations (4)