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Rayner Rodrı́guez

University of Miami

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María G. Guzmán

Instituto de Medicina Tropical “Pedro Kourí”

Mayling Álvarez

Instituto de Medicina Tropical “Pedro Kourí”

Aı́da Zulueta

Istituti Clinici Scientifici Maugeri

Ricardo Silva

Centro de Ingeniería Genética y Biotecnología

Laura Lazo

Centro de Ingeniería Genética y Biotecnología

Lisset Hermida

Zhejiang Yongning Pharma (China)

Delfina Rosario

Instituto de Medicina Tropical “Pedro Kourí”

Gerardo Guillén

Centro de Ingeniería Genética y Biotecnología

Iris Valdés

Centro de Ingeniería Genética y Biotecnología

Carlos López

Centro de Ingeniería Genética y Biotecnología

Cooperative Institutions

Instituto de Medicina Tropical “Pedro Kourí”

University of Miami

Centro de Ingeniería Genética y Biotecnología

Pan American Health Organization (Cuba)

Instituto de Medicina Tropical

National Center for Genetic Engineering and Biotechnology

Latin American School of Medicine

Instituto Politécnico Nacional

ORCID

Miami Transplant Institute

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Symmetry-projected Hartree-Fock wave functions in quantum Monte Carlo calculations

Bulletin of the American Physical Society (2014)

Hao Shi Carlos Hoyos Rayner Rodrı́guez Gustavo E. Scuseria Shiwei Zhang

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Mutagenic assessment of 1N6-ethenodeoxyadenosine in DNA

Revista latinoamericana de microbiología (1993)

Rayner Rodrı́guez Mercedes Espinosa Lara J.D Quintana Hau Sarai Luna Kenneth L. Beattie

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Citations (1)

Development of a Monoclonal Antibody Specific to a Recombinant Envelope Protein from Dengue Virus Type 4 Expressed inPichia pastoris

Hybridoma (2001)

Maritza Pupo-Antúnez Rayner Rodrı́guez Mayling Álvarez Nevys Amin Hermis Rodríguez

A mouse monoclonal antibody (MAb, 4B6) was able to recognize dengue virus type 4 envelope (E) protein both as a recombinant protein in Pichia pastoris and when it was present in infected brains of suckling mice. 4B6 was characterized by enzyme-linked immunoadsorbent assay (ELISA), hemaglutination inhibition, neutralization, and immunoblot. The MAb was isotyped as IgG2a. It was serotype 4 specific and it inhibited hemaglutination and neutralized homologous virus. It did not enhance infection of P338D1 cells by dengue type 4 virus strain H-241 strain. This MAb was reactive with recombinant E protein and dengue 4 virus, as revealed by Western blot. In vivo, MAb 4B6 conferred passive protection in mice challenged with homologous virus. Currently, this MAb is being used to purify recombinant E protein for further studies.

Flavivirus

10.1089/027245701300060409

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9238 Application of DREADD Tools Offers New Insights About the Paracrine Cellular Crosstalk in the Islet of Langerhans

Journal of the Endocrine Society (2024)

Rayner Rodrı́guez Alejandro Tamayo Dayleen Hakim Rodriguez Óscar Garnica Elizabeth Pereira

Abstract Disclosure: R. Rodriguez: None. A. Tamayo: None. D. Hakim Rodriguez: None. O. Alcazar: None. E. Pereira: None. M. Makhmutova: None. J. Almaca: None. L. Goncalves: None. The δ-cell is envisioned as a main suppressive component in the islet of Langerhans, which provides functional negative feedback to its neighboring α- and β-cells by secreting somatostatin. Considering that changes in the dynamics and amount of insulin and glucagon are associated to the natural history of Diabetes, more knowledge about the role that δ-cell and somatostatin play in the deregulation of islet glucoregulatory hormone secretion is of remarkable importance. Here we aimed to revisit the kinetics of the cellular crosstalk between β- and α-cell with the δ-cells and vice versa by taking advantage of a chemogenetic tool allowing the specific activation of any cell subset genetically modified to express DREADD (Designed Receptors Exclusively Activated by Designed Drugs). We bred mice that expressed the activator-DREADD (hM3Dq) in either α-, β-, or δ-cells. Immunohistochemical staining of pancreas sections confirmed the great cellular specificity and the effective translocation of the DREADD to the membrane of each targeted cell. Perifusion assays showed that α-, β-, and δ-cells bearing DREADD secreted glucagon, insulin and somatostatin respectively in a dose-response manner upon simulation with CNO; and as expected, the targeted stimulation of every specific cell type generated subsequent co-stimulatory or rebound responses in the other neighboring cell subsets. The data evidenced the complex network of paracrine cellular crosstalk within the islet, however the dynamics of hormone secretion in several instances did not fit into the current way we think α-, β- and δ-cells interact with each other’s. The data showed an overall increase of hormone secretion in instances we expected lessening of secretion resulting from inhibitory paracrine cellular crosstalk, such as 1) the burst of glucagon responses in non-permissive 16 mM glucose following the activation of either β- or δ-cells, and 2) net increment of insulin secretion following δ-cell activation. The results suggested more complex insights about the notion of inhibitory paracrine crosstalk within the Islet of Langerhans, where inhibition-mediated excitatory rebound responses might have more physiological relevance and be more significant for islet biology. Presentation: 6/3/2024

Crosstalk

10.1210/jendso/bvae163.668

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A fragment of the envelope protein from dengue‐1 virus, fused in two different sites of the meningococcal P64k protein carrier, induces a functional immune response in mice

Biotechnology and Applied Biochemistry (2004)

Lisset Hermida Rayner Rodrı́guez Laura Lazo Lídice Bernardo Ricardo Silva

Previously we have reported the capacity of the fusion protein PD3, composed of the P64k protein and the envelope (E) fragment from amino acids (aa) 286-426 of dengue-2 virus (DEN-2), to induce a functional immune response in mice against the homologous virus. In that case, the E fragment was inserted within the lipoyl-binding domain of the meningococcal P64k protein. In the present study, to test the functionality of the same E region from dengue-1 (DEN-1), a similar construct was made. Furthermore, another alternative of fusion protein was also constructed where the same E fragment from DEN-1 was fused to the C-terminus of the P64k protein. The recombinant proteins obtained (PD11 and PD10) were semi-purified and analysed for their antigenicity, immunogenicity and the ability to protect mice against lethal challenge. Both molecules exhibited the same recognition patterns against anti-DEN-1 polyclonal antibodies. In addition, when administered to mice, they elicited high levels of neutralizing antibodies and induced significant protection against lethal challenge with DEN-1 after intracerebral inoculation. These results reveal the availability of two sites within the P64k for the further insertion of DEN fragments, enabling a construct carrying two fragments from heterologous serotypes within the same molecule of this protein carrier.

Antigenicity

Dengue vaccine

Polyclonal antibodies

Flavivirus

10.1042/ba20030039

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Citations (33)

The fusion site of envelope fragments from each serotype of Dengue virus in the P64k protein, influence some parameters of the resulting chimeric constructs

Biochemical and Biophysical Research Communications (2003)

Aı́da Zulueta Lisset Hermida Laura Lazo Iris Valdés Rayner Rodrı́guez

Dengue vaccine

Envelope (radar)

Flavivirus

10.1016/s0006-291x(03)01411-6

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Citations (25)

Potencialidad inmunogénica de la proteína de la envoltura del virus dengue 4 expresada por vía recombinante en Pichia pastoris

Biotecnología aplicada (2004)

Mayra Muné Rayner Rodrı́guez Guadalupe Guzmán Rosa Ramírez R. Martinez Rodriguez

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Long-term memory cellular immune response to dengue virus after a natural primary infection

International Journal of Infectious Diseases (2002)

Beatríz Sierra Gissel Garcı́a Ana Beatriz Alvarez Pérez Luís Morier Rayner Rodrı́guez

This study was conducted to examine the memory T-cell response to dengue virus 20 years after a primary infection. We took advantage of the exceptional epidemiologic situation in Cuba, where the population initially suffered two large successive epidemics due to dengue virus 1 and 2 respectively over a 4-year period. Thereafter, no dengue virus circulation was subsequently observed, except for the Santiago de Cuba municipality.T-cell response was evaluated in peripheral blood mononuclear cells (PBMCs) from 20 individuals with history of a primary infection by dengue virus 1 or 2. Methods previously shown to induce lymphoproliferation of CD4+ memory T-cell subpopulations were used. We evaluated the proliferative responses generated in those PBMCs after stimulation with dengue virus 1, 2, 3 and 4 antigens in a serotype-specific and serotype-crossreactive way.Serotype-specific and serotype-crossreactive lymphoproliferative responses in all PBMCs donated by dengue immune donors were observed. The serotype-crossreactive response for dengue 2 was stronger than for the rest of the serotypes.This is the first report of cellular memory lymphocyte response specific for dengue virus detected 20 years after a primary infection by dengue.

Flavivirus

Original antigenic sin

10.1016/s1201-9712(02)90073-1

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Citations (46)

A dengue-2 Envelope fragment inserted within the structure of the P64k meningococcal protein carrier enables a functional immune response against the virus in mice

Journal of Virological Methods (2003)

Lisset Hermida Rayner Rodrı́guez Laura Lazo Ricardo Silva Aı́da Zulueta

Envelope (radar)

10.1016/j.jviromet.2003.09.024

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Citations (55)

Human Dengue Antibodies against Structural and Nonstructural Proteins

Clinical and Diagnostic Laboratory Immunology (2000)

Katia Valdés Mayling Álvarez Maritza Pupo Susana Vázquez Rayner Rodrı́guez

ABSTRACT Antibodies against dengue virus type 2 and 4 proteins in acute-phase sera of 10 primary and 10 secondary dengue fever and dengue hemorrhagic fever patients were studied by Western blotting. In the first group the immune response was barely detectable, while in the second group more proteins were detected, with a very strong reaction. Anti-NS1 and -NS3 antibodies were detected mainly in secondary cases. Anti-E, -NS3, and -NS5 antibodies were detected in a high number of cases. The possibility of implementing early diagnostic assays for antigen detection is suggested.

NS3

Structural protein

10.1128/cdli.7.5.856-857.2000

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Citations (117)